Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. / Azzam, Ramzi; Chen, Susan L.; Shou, Wenying; Mah, Angie S.; Alexandru, Gabriela; Nasmyth, Kim; Annan, Roland S.; Carr, Steven A.; Deshaies, Raymond J.
In: Science, Vol. 305, No. 5683, 2004, p. 516-519.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus
A1 - Azzam,Ramzi
A1 - Chen,Susan L.
A1 - Shou,Wenying
A1 - Mah,Angie S.
A1 - Alexandru,Gabriela
A1 - Nasmyth,Kim
A1 - Annan,Roland S.
A1 - Carr,Steven A.
A1 - Deshaies,Raymond J.
AU - Azzam,Ramzi
AU - Chen,Susan L.
AU - Shou,Wenying
AU - Mah,Angie S.
AU - Alexandru,Gabriela
AU - Nasmyth,Kim
AU - Annan,Roland S.
AU - Carr,Steven A.
AU - Deshaies,Raymond J.
PY - 2004
Y1 - 2004
N2 - <p>Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.</p>
AB - <p>Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.</p>
U2 - 10.1126/science.1099402
DO - 10.1126/science.1099402
M1 - Article
JO - Science
JF - Science
SN - 0036-8075
IS - 5683
VL - 305
SP - 516
EP - 519
ER -