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Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

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Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance. / Riggio, Cristina; Pilar Calatayud, Maria; Hoskins, Clare; Pinkernelle, Josephine; Sanz, Beatriz; Enrique Torres, Teobaldo; Ricardo Ibarra, Manuel; Wang, Lijun; Keilhoff, Gerburg; Fabian Goya, Gerardo; Raffa, Vittoria; Cuschieri, Alfred.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 3155-3166.

Research output: Contribution to journalArticle

Harvard

Riggio, C, Pilar Calatayud, M, Hoskins, C, Pinkernelle, J, Sanz, B, Enrique Torres, T, Ricardo Ibarra, M, Wang, L, Keilhoff, G, Fabian Goya, G, Raffa, V & Cuschieri, A 2012, 'Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance' International Journal of Nanomedicine, vol 7, pp. 3155-3166., 10.2147/IJN.S28460

APA

Riggio, C., Pilar Calatayud, M., Hoskins, C., Pinkernelle, J., Sanz, B., Enrique Torres, T., ... Cuschieri, A. (2012). Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance. International Journal of Nanomedicine, 7, 3155-3166. 10.2147/IJN.S28460

Vancouver

Riggio C, Pilar Calatayud M, Hoskins C, Pinkernelle J, Sanz B, Enrique Torres T et al. Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance. International Journal of Nanomedicine. 2012;7:3155-3166. Available from: 10.2147/IJN.S28460

Author

Riggio, Cristina; Pilar Calatayud, Maria; Hoskins, Clare; Pinkernelle, Josephine; Sanz, Beatriz; Enrique Torres, Teobaldo; Ricardo Ibarra, Manuel; Wang, Lijun; Keilhoff, Gerburg; Fabian Goya, Gerardo; Raffa, Vittoria; Cuschieri, Alfred / Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 3155-3166.

Research output: Contribution to journalArticle

Bibtex - Download

@article{a9d51c3e2f2b4420929690d89d7d1296,
title = "Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance",
author = "Cristina Riggio and {Pilar Calatayud}, Maria and Clare Hoskins and Josephine Pinkernelle and Beatriz Sanz and {Enrique Torres}, Teobaldo and {Ricardo Ibarra}, Manuel and Lijun Wang and Gerburg Keilhoff and {Fabian Goya}, Gerardo and Vittoria Raffa and Alfred Cuschieri",
year = "2012",
doi = "10.2147/IJN.S28460",
volume = "7",
pages = "3155--3166",
journal = "International Journal of Nanomedicine",
issn = "1178-2013",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

A1 - Riggio,Cristina

A1 - Pilar Calatayud,Maria

A1 - Hoskins,Clare

A1 - Pinkernelle,Josephine

A1 - Sanz,Beatriz

A1 - Enrique Torres,Teobaldo

A1 - Ricardo Ibarra,Manuel

A1 - Wang,Lijun

A1 - Keilhoff,Gerburg

A1 - Fabian Goya,Gerardo

A1 - Raffa,Vittoria

A1 - Cuschieri,Alfred

AU - Riggio,Cristina

AU - Pilar Calatayud,Maria

AU - Hoskins,Clare

AU - Pinkernelle,Josephine

AU - Sanz,Beatriz

AU - Enrique Torres,Teobaldo

AU - Ricardo Ibarra,Manuel

AU - Wang,Lijun

AU - Keilhoff,Gerburg

AU - Fabian Goya,Gerardo

AU - Raffa,Vittoria

AU - Cuschieri,Alfred

PY - 2012

Y1 - 2012

N2 - <p>Purpose: It has been proposed in the literature that Fe3O4 magnetic nanoparticles (MNPs) could be exploited to enhance or accelerate nerve regeneration and to provide guidance for regenerating axons. MNPs could create mechanical tension that stimulates the growth and elongation of axons. Particles suitable for this purpose should possess (1) high saturation magnetization, (2) a negligible cytotoxic profile, and (3) a high capacity to magnetize mammalian cells. Unfortunately, the materials currently available on the market do not satisfy these criteria; therefore, this work attempts to overcome these deficiencies.</p><p>Methods: Magnetite particles were synthesized by an oxidative hydrolysis method and characterized based on their external morphology and size distribution (high-resolution transmission electron microscopy [HR-TEM]) as well as their colloidal (Z potential) and magnetic properties (Superconducting QUantum Interference Devices [SQUID]). Cell viability was assessed via Trypan blue dye exclusion assay, cell doubling time, and MTT cell proliferation assay and reactive oxygen species production. Particle uptake was monitored via Prussian blue staining, intracellular iron content quantification via a ferrozine-based assay, and direct visualization by dual-beam (focused ion beam/scanning electron microscopy [FIB/SEM]) analysis. Experiments were performed on human neuroblastoma SH-SY5Y cell line and primary Schwann cell cultures of the peripheral nervous system.</p><p>Results: This paper reports on the synthesis and characterization of polymer-coated magnetic Fe3O4 nanoparticles with an average diameter of 73 +/- 6 nm that are designed as magnetic actuators for neural guidance. The cells were able to incorporate quantities of iron up to 2 pg/cell. The intracellular distribution of MNPs obtained by optical and electronic microscopy showed large structures of MNPs crossing the cell membrane into the cytoplasm, thus rendering them suitable for magnetic manipulation by external magnetic fields. Specifically, migration experiments under external magnetic fields confirmed that these MNPs can effectively actuate the cells, thus inducing measurable migration towards predefined directions more effectively than commercial nanoparticles (fluidMAG-ARA supplied by Chemicell). There were no observable toxic effects from MNPs on cell viability for working concentrations of 10 mu g/mL (EC25 of 20.8 mu g/mL, compared to 12 mu g/mL in fluidMAG-ARA). Cell proliferation assays performed with primary cell cultures of the peripheral nervous system confirmed moderate cytotoxicity (EC25 of 10.35 mu g/mL).</p><p>Conclusion: These results indicate that loading neural cells with the proposed MNPs is likely to be an effective strategy for promoting non- invasive neural regeneration through cell magnetic actuation.</p>

AB - <p>Purpose: It has been proposed in the literature that Fe3O4 magnetic nanoparticles (MNPs) could be exploited to enhance or accelerate nerve regeneration and to provide guidance for regenerating axons. MNPs could create mechanical tension that stimulates the growth and elongation of axons. Particles suitable for this purpose should possess (1) high saturation magnetization, (2) a negligible cytotoxic profile, and (3) a high capacity to magnetize mammalian cells. Unfortunately, the materials currently available on the market do not satisfy these criteria; therefore, this work attempts to overcome these deficiencies.</p><p>Methods: Magnetite particles were synthesized by an oxidative hydrolysis method and characterized based on their external morphology and size distribution (high-resolution transmission electron microscopy [HR-TEM]) as well as their colloidal (Z potential) and magnetic properties (Superconducting QUantum Interference Devices [SQUID]). Cell viability was assessed via Trypan blue dye exclusion assay, cell doubling time, and MTT cell proliferation assay and reactive oxygen species production. Particle uptake was monitored via Prussian blue staining, intracellular iron content quantification via a ferrozine-based assay, and direct visualization by dual-beam (focused ion beam/scanning electron microscopy [FIB/SEM]) analysis. Experiments were performed on human neuroblastoma SH-SY5Y cell line and primary Schwann cell cultures of the peripheral nervous system.</p><p>Results: This paper reports on the synthesis and characterization of polymer-coated magnetic Fe3O4 nanoparticles with an average diameter of 73 +/- 6 nm that are designed as magnetic actuators for neural guidance. The cells were able to incorporate quantities of iron up to 2 pg/cell. The intracellular distribution of MNPs obtained by optical and electronic microscopy showed large structures of MNPs crossing the cell membrane into the cytoplasm, thus rendering them suitable for magnetic manipulation by external magnetic fields. Specifically, migration experiments under external magnetic fields confirmed that these MNPs can effectively actuate the cells, thus inducing measurable migration towards predefined directions more effectively than commercial nanoparticles (fluidMAG-ARA supplied by Chemicell). There were no observable toxic effects from MNPs on cell viability for working concentrations of 10 mu g/mL (EC25 of 20.8 mu g/mL, compared to 12 mu g/mL in fluidMAG-ARA). Cell proliferation assays performed with primary cell cultures of the peripheral nervous system confirmed moderate cytotoxicity (EC25 of 10.35 mu g/mL).</p><p>Conclusion: These results indicate that loading neural cells with the proposed MNPs is likely to be an effective strategy for promoting non- invasive neural regeneration through cell magnetic actuation.</p>

U2 - 10.2147/IJN.S28460

DO - 10.2147/IJN.S28460

M1 - Article

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1178-2013

VL - 7

SP - 3155

EP - 3166

ER -

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