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Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells

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Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells. / Navarro, Maria N.; Sinclair, Linda V.; Feijoo-Carnero, Carmen; Clarke, Rosemary; Matthews, Sharon A.; Cantrell, Doreen A.

In: Biochemical Journal, Vol. 442, No. 3, 15.03.2012, p. 649-659.

Research output: Contribution to journalArticle

Harvard

Navarro, MN, Sinclair, LV, Feijoo-Carnero, C, Clarke, R, Matthews, SA & Cantrell, DA 2012, 'Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells' Biochemical Journal, vol 442, no. 3, pp. 649-659.

APA

Navarro, M. N., Sinclair, L. V., Feijoo-Carnero, C., Clarke, R., Matthews, S. A., & Cantrell, D. A. (2012). Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells. Biochemical Journal, 442(3), 649-659doi: 10.1042/BJ20111700

Vancouver

Navarro MN, Sinclair LV, Feijoo-Carnero C, Clarke R, Matthews SA, Cantrell DA. Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells. Biochemical Journal. 2012 Mar 15;442(3):649-659.

Author

Navarro, Maria N.; Sinclair, Linda V.; Feijoo-Carnero, Carmen; Clarke, Rosemary; Matthews, Sharon A.; Cantrell, Doreen A. / Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells.

In: Biochemical Journal, Vol. 442, No. 3, 15.03.2012, p. 649-659.

Research output: Contribution to journalArticle

Bibtex - Download

@article{0f7707bda3ec4e818a647d1f7c990742,
title = "Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells",
author = "Navarro, {Maria N.} and Sinclair, {Linda V.} and Carmen Feijoo-Carnero and Rosemary Clarke and Matthews, {Sharon A.} and Cantrell, {Doreen A.}",
year = "2012",
volume = "442",
number = "3",
pages = "649--659",
journal = "Biochemical Journal",
issn = "0264-6021",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells

A1 - Navarro,Maria N.

A1 - Sinclair,Linda V.

A1 - Feijoo-Carnero,Carmen

A1 - Clarke,Rosemary

A1 - Matthews,Sharon A.

A1 - Cantrell,Doreen A.

AU - Navarro,Maria N.

AU - Sinclair,Linda V.

AU - Feijoo-Carnero,Carmen

AU - Clarke,Rosemary

AU - Matthews,Sharon A.

AU - Cantrell,Doreen A.

PY - 2012/3/15

Y1 - 2012/3/15

N2 - PKD (protein kinase D) 2 is a serine/threonine kinase activated by diacylglycerol in response to engagement of antigen receptors in lymphocytes. To explore PKD2 regulation and function in TCR (T-cell antigen receptor) signal transduction we expressed TCR complexes with fixed affinity for self antigens in the T-cells of PKD2-null mice or mice deficient in PKD2 catalytic activity.We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engagement of a/ß TCR complexes in vivo. Strikingly, PKD2 loss caused increases in thymic output, lymphadenopathy and splenomegaly in TCR transgenic mice. The precise magnitude and timing of PKD2 activation during T-cell development is thus critical to regulate thymic homoeostasis. PKD2-null T-cells that exit the thymus have a normal transcriptome, but show a limited and abnormal transcriptional response to antigen. Transcriptional profiling reveals the full consequences of PKD2 loss and maps in detail the selective, but critical, function for PKD2 in signalling by a/ß mature TCR complexes in peripheral T-cells. © The Authors Journal compilation © 2012 Biochemical Society.

AB - PKD (protein kinase D) 2 is a serine/threonine kinase activated by diacylglycerol in response to engagement of antigen receptors in lymphocytes. To explore PKD2 regulation and function in TCR (T-cell antigen receptor) signal transduction we expressed TCR complexes with fixed affinity for self antigens in the T-cells of PKD2-null mice or mice deficient in PKD2 catalytic activity.We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engagement of a/ß TCR complexes in vivo. Strikingly, PKD2 loss caused increases in thymic output, lymphadenopathy and splenomegaly in TCR transgenic mice. The precise magnitude and timing of PKD2 activation during T-cell development is thus critical to regulate thymic homoeostasis. PKD2-null T-cells that exit the thymus have a normal transcriptome, but show a limited and abnormal transcriptional response to antigen. Transcriptional profiling reveals the full consequences of PKD2 loss and maps in detail the selective, but critical, function for PKD2 in signalling by a/ß mature TCR complexes in peripheral T-cells. © The Authors Journal compilation © 2012 Biochemical Society.

KW - Cytokine

KW - Lymphocyte

KW - Pre-cell antigen receptor

KW - Protein kinase D (PKD)

KW - T-cell antigen receptor (TCR)

KW - Thymus

UR - http://ukpmc.ac.uk/abstract/MED/22233340

U2 - 10.1042/BJ20111700

DO - 10.1042/BJ20111700

M1 - Article

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

VL - 442

SP - 649

EP - 659

ER -

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