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Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway

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Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. / Bruce, David L.; Macartney, Thomas; Yong, Weidong; Shou, Weinian; Sapkota, Gopal P. (Lead / Corresponding author).

In: Cellular Signalling, Vol. 24, No. 11, 11.2012, p. 1999-2006.

Research output: Contribution to journalArticle

Harvard

Bruce, DL, Macartney, T, Yong, W, Shou, W & Sapkota, GP 2012, 'Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway' Cellular Signalling, vol 24, no. 11, pp. 1999-2006., 10.1016/j.cellsig.2012.07.003

APA

Bruce, D. L., Macartney, T., Yong, W., Shou, W., & Sapkota, G. P. (2012). Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. Cellular Signalling, 24(11), 1999-2006. 10.1016/j.cellsig.2012.07.003

Vancouver

Bruce DL, Macartney T, Yong W, Shou W, Sapkota GP. Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. Cellular Signalling. 2012 Nov;24(11):1999-2006. Available from: 10.1016/j.cellsig.2012.07.003

Author

Bruce, David L.; Macartney, Thomas; Yong, Weidong; Shou, Weinian; Sapkota, Gopal P. (Lead / Corresponding author) / Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway.

In: Cellular Signalling, Vol. 24, No. 11, 11.2012, p. 1999-2006.

Research output: Contribution to journalArticle

Bibtex - Download

@article{44725aa43a244c74a99ad35db677656a,
title = "Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway",
author = "Bruce, {David L.} and Thomas Macartney and Weidong Yong and Weinian Shou and Sapkota, {Gopal P.}",
year = "2012",
doi = "10.1016/j.cellsig.2012.07.003",
volume = "24",
number = "11",
pages = "1999--2006",
journal = "Cellular Signalling",
issn = "0898-6568",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway

A1 - Bruce,David L.

A1 - Macartney,Thomas

A1 - Yong,Weidong

A1 - Shou,Weinian

A1 - Sapkota,Gopal P.

AU - Bruce,David L.

AU - Macartney,Thomas

AU - Yong,Weidong

AU - Shou,Weinian

AU - Sapkota,Gopal P.

PY - 2012/11

Y1 - 2012/11

N2 - <p>Protein phosphatases play a key role in balancing the cellular responses to the transforming growth factor-beta (TGF beta) signals. Several protein phosphatases have been attributed roles in the regulation of the TGF beta pathway. Among these. PPM1A is the only phosphatase reported to dephosphorylate SMAD2/3 in the nucleus. However we observed PPM1A exclusively in the cytoplasmic fractions independently of TOM treatment in all cells tested. These observations imply that a bona fide nuclear SMAD2/3 phosphatase remains elusive. In this study, we report a role for protein phosphatase 5 (PP5) in the TGF beta pathway. We identified PP5 as an interactor of SMAD2/3. Interestingly, in mouse embryonic fibroblast cells derived from PP5-null mice, TGF beta-induced transcriptional responses were significantly enhanced. Rather surprisingly, this enhancement is due to the increased levels of SMAD3 protein observed in PP5-null MEFs compared to the wild type. No differences in the levels of SMAD3 transcripts were observed between the wild-type and PPS-null MEFs. While PP5 is capable of dephosphoiylating SMAD3-tail in overexpression assays, we demonstrate that its activity is essential in controlling SMAD3 protein levels in MEFs. We propose that PP5 regulates the TGF beta pathway in MEFs by regulating the expression of SMAD3 protein levels. (C) 2012 Elsevier Inc. All rights reserved.</p> <p>NOTICE: this is the author’s version of a work that was accepted for publication in Cellular Signalling. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cellular Signalling, [VOL.24, ISSUE 11, (2012)] DOI 10.1016/j.cellsig.2012.07.003</p>

AB - <p>Protein phosphatases play a key role in balancing the cellular responses to the transforming growth factor-beta (TGF beta) signals. Several protein phosphatases have been attributed roles in the regulation of the TGF beta pathway. Among these. PPM1A is the only phosphatase reported to dephosphorylate SMAD2/3 in the nucleus. However we observed PPM1A exclusively in the cytoplasmic fractions independently of TOM treatment in all cells tested. These observations imply that a bona fide nuclear SMAD2/3 phosphatase remains elusive. In this study, we report a role for protein phosphatase 5 (PP5) in the TGF beta pathway. We identified PP5 as an interactor of SMAD2/3. Interestingly, in mouse embryonic fibroblast cells derived from PP5-null mice, TGF beta-induced transcriptional responses were significantly enhanced. Rather surprisingly, this enhancement is due to the increased levels of SMAD3 protein observed in PP5-null MEFs compared to the wild type. No differences in the levels of SMAD3 transcripts were observed between the wild-type and PPS-null MEFs. While PP5 is capable of dephosphoiylating SMAD3-tail in overexpression assays, we demonstrate that its activity is essential in controlling SMAD3 protein levels in MEFs. We propose that PP5 regulates the TGF beta pathway in MEFs by regulating the expression of SMAD3 protein levels. (C) 2012 Elsevier Inc. All rights reserved.</p> <p>NOTICE: this is the author’s version of a work that was accepted for publication in Cellular Signalling. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cellular Signalling, [VOL.24, ISSUE 11, (2012)] DOI 10.1016/j.cellsig.2012.07.003</p>

U2 - 10.1016/j.cellsig.2012.07.003

DO - 10.1016/j.cellsig.2012.07.003

M1 - Article

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 11

VL - 24

SP - 1999

EP - 2006

ER -

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    NOTICE: this is the author’s version of a work that was accepted for publication in Cellular Signalling. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cellular Signalling, [VOL.24, ISSUE 11, (2012)] DOI 10.1016/j.cellsig.2012.07.003

DOI

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