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Purification and identification of endogenous polySUMO conjugates

Purification and identification of endogenous polySUMO conjugates

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Authors

  • Roland Bruderer
  • Michael H. Tatham
  • Anna Plechanovova
  • Ivan Matic
  • Amit K. Garg
  • Ronald T. Hay

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Info

Original languageEnglish
Pages142-148
Number of pages7
JournalEMBO Reports
Journal publication dateFeb 2011
Volume12
Issue2
DOIs
StatePublished

Abstract

The small ubiquitin-like modifier (SUMO) can undergo self-modification to form polymeric chains that have been implicated in cellular processes such as meiosis, genome maintenance and stress response. Investigations into the biological role of polymeric chains have been hampered by the absence of a protocol for the purification of proteins linked to SUMO chains. In this paper, we describe a rapid affinity purification procedure for the isolation of endogenous polySUMO-modified species that generates highly purified material suitable for individual protein studies and proteomic analysis. We use this approach to identify more than 300 putative polySUMO conjugates from cultured eukaryotic cells.

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