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Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms

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Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms. / Hockin, Simon; Gadd, Geoffrey M.

In: Environmental Microbiology, Vol. 8, No. 5, 05.2006, p. 816-826.

Research output: Contribution to journalArticle

Harvard

Hockin, S & Gadd, GM 2006, 'Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms' Environmental Microbiology, vol 8, no. 5, pp. 816-826.

APA

Hockin, S., & Gadd, G. M. (2006). Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms. Environmental Microbiology, 8(5), 816-826doi: 10.1111/j.1462-2920.2005.00967.x

Vancouver

Hockin S, Gadd GM. Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms. Environmental Microbiology. 2006 May;8(5):816-826.

Author

Hockin, Simon; Gadd, Geoffrey M. / Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms.

In: Environmental Microbiology, Vol. 8, No. 5, 05.2006, p. 816-826.

Research output: Contribution to journalArticle

Bibtex - Download

@article{b329f8ae976041e0b3176a929cca86b6,
title = "Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms",
author = "Simon Hockin and Gadd, {Geoffrey M.}",
note = "dc.publisher: Wiley-Blackwell",
year = "2006",
volume = "8",
number = "5",
pages = "816--826",
journal = "Environmental Microbiology",
issn = "1462-2912",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Removal of selenate from sulfate-containing media by sulfate-reducing bacterial biofilms

A1 - Hockin,Simon

A1 - Gadd,Geoffrey M.

AU - Hockin,Simon

AU - Gadd,Geoffrey M.

PY - 2006/5

Y1 - 2006/5

N2 - A biofilm-selected strain of a Desulfomicrobium sp. removed selenate from solution to sub-micromolar concentrations during growth on lactate (or hydrogen) and sulfate. Under sulfate-limited growth conditions, selenium was enzymatically reduced to selenide. Under excess sulfate conditions, selenate removal was primarily by enzymatic reduction to elemental selenium. Sequestration by biofilms was greater under the latter condition. Experiments with washed cell suspensions showed that high sulfate concentrations inhibited cell-specific selenate reduction, but when growing cells were exposed to selenate, the biomass increase achieved during incubations with abundant sulfate resulted in more rapid selenate removal. The addition of small amounts of sulfite, or thiosulfate, ameliorated this inhibition. Nitrate also inhibited selenate reduction in washed cell suspensions, apparently due to a general oxidizing effect. These results suggest that where biofilm-based sulfate-reducing bacteria (SRB) bioreactors are considered for the treatment of mixed metalliferous wastes that contain selenium oxyanions, adequate selenate removal should be achievable under a range of environmental conditions. The form and fate of the precipitated product will, however, be influenced by the dominant reduction pathway, which is controlled by environmental variables.

AB - A biofilm-selected strain of a Desulfomicrobium sp. removed selenate from solution to sub-micromolar concentrations during growth on lactate (or hydrogen) and sulfate. Under sulfate-limited growth conditions, selenium was enzymatically reduced to selenide. Under excess sulfate conditions, selenate removal was primarily by enzymatic reduction to elemental selenium. Sequestration by biofilms was greater under the latter condition. Experiments with washed cell suspensions showed that high sulfate concentrations inhibited cell-specific selenate reduction, but when growing cells were exposed to selenate, the biomass increase achieved during incubations with abundant sulfate resulted in more rapid selenate removal. The addition of small amounts of sulfite, or thiosulfate, ameliorated this inhibition. Nitrate also inhibited selenate reduction in washed cell suspensions, apparently due to a general oxidizing effect. These results suggest that where biofilm-based sulfate-reducing bacteria (SRB) bioreactors are considered for the treatment of mixed metalliferous wastes that contain selenium oxyanions, adequate selenate removal should be achievable under a range of environmental conditions. The form and fate of the precipitated product will, however, be influenced by the dominant reduction pathway, which is controlled by environmental variables.

U2 - 10.1111/j.1462-2920.2005.00967.x

DO - 10.1111/j.1462-2920.2005.00967.x

M1 - Article

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 5

VL - 8

SP - 816

EP - 826

ER -

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