Discovery - University of Dundee - Online Publications

Library & Learning Centre

RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle

Standard

RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. / Albert, Benjamin; Leger-Silvestre, Isabelle; Normand, Christophe; Ostermaier, Martin K.; Perez-Fernandez, Jorge; Panov, Kostya I.; Zomerdijk, Joost C. B. M.; Schultz, Patrick; Gadal, Olivier (Lead / Corresponding author).

In: Journal of Cell Biology, Vol. 192, No. 2, 24.01.2011, p. 277-293.

Research output: Contribution to journalArticle

Harvard

Albert, B, Leger-Silvestre, I, Normand, C, Ostermaier, MK, Perez-Fernandez, J, Panov, KI, Zomerdijk, JCBM, Schultz, P & Gadal, O 2011, 'RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle' Journal of Cell Biology, vol 192, no. 2, pp. 277-293.

APA

Albert, B., Leger-Silvestre, I., Normand, C., Ostermaier, M. K., Perez-Fernandez, J., Panov, K. I., Zomerdijk, J. C. B. M., Schultz, P., & Gadal, O. (2011). RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. Journal of Cell Biology, 192(2), 277-293doi: 10.1083/jcb.201006040

Vancouver

Albert B, Leger-Silvestre I, Normand C, Ostermaier MK, Perez-Fernandez J, Panov KI et al. RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. Journal of Cell Biology. 2011 Jan 24;192(2):277-293.

Author

Albert, Benjamin; Leger-Silvestre, Isabelle; Normand, Christophe; Ostermaier, Martin K.; Perez-Fernandez, Jorge; Panov, Kostya I.; Zomerdijk, Joost C. B. M.; Schultz, Patrick; Gadal, Olivier (Lead / Corresponding author) / RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle.

In: Journal of Cell Biology, Vol. 192, No. 2, 24.01.2011, p. 277-293.

Research output: Contribution to journalArticle

Bibtex - Download

@article{76fcefe94d9f44239d269e85cd1ffefe,
title = "RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle",
author = "Benjamin Albert and Isabelle Leger-Silvestre and Christophe Normand and Ostermaier, {Martin K.} and Jorge Perez-Fernandez and Panov, {Kostya I.} and Zomerdijk, {Joost C. B. M.} and Patrick Schultz and Olivier Gadal",
year = "2011",
volume = "192",
number = "2",
pages = "277--293",
journal = "Journal of Cell Biology",
issn = "0021-9525",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle

A1 - Albert,Benjamin

A1 - Leger-Silvestre,Isabelle

A1 - Normand,Christophe

A1 - Ostermaier,Martin K.

A1 - Perez-Fernandez,Jorge

A1 - Panov,Kostya I.

A1 - Zomerdijk,Joost C. B. M.

A1 - Schultz,Patrick

A1 - Gadal,Olivier

AU - Albert,Benjamin

AU - Leger-Silvestre,Isabelle

AU - Normand,Christophe

AU - Ostermaier,Martin K.

AU - Perez-Fernandez,Jorge

AU - Panov,Kostya I.

AU - Zomerdijk,Joost C. B. M.

AU - Schultz,Patrick

AU - Gadal,Olivier

PY - 2011/1/24

Y1 - 2011/1/24

N2 - <p>RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol I subunits, which do not have counterparts in Pol II and Pol III complexes, are functionally conserved using heterospecific complementation of the human and Schizosaccharomyces pombe orthologues in Saccharomyces cerevisiae. Deletion of RPA49 leads to the disappearance of nucleolar structure, but nucleolar assembly can be restored by decreasing ribosomal gene copy number from 190 to 25. Statistical analysis of Miller spreads in the absence of Rpa49 demonstrates a fourfold decrease in Pol I loading rate per gene and decreased contact between adjacent Pol I complexes. Therefore, the Rpa34 and Rpa49 Poll specific subunits are essential for nucleolar assembly and for the high polynnerase loading rate associated with frequent contact between adjacent enzymes. Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly.</p> <p><strong>© 2011 Albert et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).</strong></p>

AB - <p>RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol I subunits, which do not have counterparts in Pol II and Pol III complexes, are functionally conserved using heterospecific complementation of the human and Schizosaccharomyces pombe orthologues in Saccharomyces cerevisiae. Deletion of RPA49 leads to the disappearance of nucleolar structure, but nucleolar assembly can be restored by decreasing ribosomal gene copy number from 190 to 25. Statistical analysis of Miller spreads in the absence of Rpa49 demonstrates a fourfold decrease in Pol I loading rate per gene and decreased contact between adjacent Pol I complexes. Therefore, the Rpa34 and Rpa49 Poll specific subunits are essential for nucleolar assembly and for the high polynnerase loading rate associated with frequent contact between adjacent enzymes. Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly.</p> <p><strong>© 2011 Albert et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).</strong></p>

KW - SACCHAROMYCES-CEREVISIAE

KW - PROTEIN INTERACTIONS

KW - A49 SUBUNIT

KW - YEAST

KW - DNA

KW - NUCLEOLUS

KW - BINDING

KW - MUTANTS

KW - VISUALIZATION

KW - LOCALIZATION

U2 - 10.1083/jcb.201006040

DO - 10.1083/jcb.201006040

M1 - Article

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

VL - 192

SP - 277

EP - 293

ER -

Documents

Documents

DOI

Library & Learning Centre

Contact | Accessibility | Policy