RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. / Albert, Benjamin; Leger-Silvestre, Isabelle; Normand, Christophe; Ostermaier, Martin K.; Perez-Fernandez, Jorge; Panov, Kostya I.; Zomerdijk, Joost C. B. M.; Schultz, Patrick; Gadal, Olivier.
In: Journal of Cell Biology, Vol. 192, No. 2, 24.01.2011, p. 277-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
A1 - Albert,Benjamin
A1 - Leger-Silvestre,Isabelle
A1 - Normand,Christophe
A1 - Ostermaier,Martin K.
A1 - Perez-Fernandez,Jorge
A1 - Panov,Kostya I.
A1 - Zomerdijk,Joost C. B. M.
A1 - Schultz,Patrick
A1 - Gadal,Olivier
AU - Albert,Benjamin
AU - Leger-Silvestre,Isabelle
AU - Normand,Christophe
AU - Ostermaier,Martin K.
AU - Perez-Fernandez,Jorge
AU - Panov,Kostya I.
AU - Zomerdijk,Joost C. B. M.
AU - Schultz,Patrick
AU - Gadal,Olivier
PY - 2011/1/24
Y1 - 2011/1/24
N2 - <p>RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol I subunits, which do not have counterparts in Pol II and Pol III complexes, are functionally conserved using heterospecific complementation of the human and Schizosaccharomyces pombe orthologues in Saccharomyces cerevisiae. Deletion of RPA49 leads to the disappearance of nucleolar structure, but nucleolar assembly can be restored by decreasing ribosomal gene copy number from 190 to 25. Statistical analysis of Miller spreads in the absence of Rpa49 demonstrates a fourfold decrease in Pol I loading rate per gene and decreased contact between adjacent Pol I complexes. Therefore, the Rpa34 and Rpa49 Poll specific subunits are essential for nucleolar assembly and for the high polynnerase loading rate associated with frequent contact between adjacent enzymes. Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly.</p>
AB - <p>RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol I subunits, which do not have counterparts in Pol II and Pol III complexes, are functionally conserved using heterospecific complementation of the human and Schizosaccharomyces pombe orthologues in Saccharomyces cerevisiae. Deletion of RPA49 leads to the disappearance of nucleolar structure, but nucleolar assembly can be restored by decreasing ribosomal gene copy number from 190 to 25. Statistical analysis of Miller spreads in the absence of Rpa49 demonstrates a fourfold decrease in Pol I loading rate per gene and decreased contact between adjacent Pol I complexes. Therefore, the Rpa34 and Rpa49 Poll specific subunits are essential for nucleolar assembly and for the high polynnerase loading rate associated with frequent contact between adjacent enzymes. Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly.</p>
KW - SACCHAROMYCES-CEREVISIAE
KW - PROTEIN INTERACTIONS
KW - A49 SUBUNIT
KW - YEAST
KW - DNA
KW - NUCLEOLUS
KW - BINDING
KW - MUTANTS
KW - VISUALIZATION
KW - LOCALIZATION
U2 - 10.1083/jcb.201006040
DO - 10.1083/jcb.201006040
M1 - Article
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 2
VL - 192
SP - 277
EP - 293
ER -