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Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis

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Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. / Posch, Markus; Khoudoli, Guennadi A.; Swift, Sam; King, Emma M.; DeLuca, Jennifer G.; Swedlow, Jason R. (Lead / Corresponding author).

In: Journal of Cell Biology, Vol. 191, No. 1, 04.10.2010, p. 61-74.

Research output: Contribution to journalArticle

Harvard

Posch, M, Khoudoli, GA, Swift, S, King, EM, DeLuca, JG & Swedlow, JR 2010, 'Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis' Journal of Cell Biology, vol 191, no. 1, pp. 61-74.

APA

Posch, M., Khoudoli, G. A., Swift, S., King, E. M., DeLuca, J. G., & Swedlow, J. R. (2010). Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. Journal of Cell Biology, 191(1), 61-74doi: 10.1083/jcb.200912046

Vancouver

Posch M, Khoudoli GA, Swift S, King EM, DeLuca JG, Swedlow JR. Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. Journal of Cell Biology. 2010 Oct 4;191(1):61-74.

Author

Posch, Markus; Khoudoli, Guennadi A.; Swift, Sam; King, Emma M.; DeLuca, Jennifer G.; Swedlow, Jason R. (Lead / Corresponding author) / Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis.

In: Journal of Cell Biology, Vol. 191, No. 1, 04.10.2010, p. 61-74.

Research output: Contribution to journalArticle

Bibtex - Download

@article{2ba3154da54249f48e2659134e412a6a,
title = "Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis",
author = "Markus Posch and Khoudoli, {Guennadi A.} and Sam Swift and King, {Emma M.} and DeLuca, {Jennifer G.} and Swedlow, {Jason R.}",
year = "2010",
volume = "191",
number = "1",
pages = "61--74",
journal = "Journal of Cell Biology",
issn = "0021-9525",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis

A1 - Posch,Markus

A1 - Khoudoli,Guennadi A.

A1 - Swift,Sam

A1 - King,Emma M.

A1 - DeLuca,Jennifer G.

A1 - Swedlow,Jason R.

AU - Posch,Markus

AU - Khoudoli,Guennadi A.

AU - Swift,Sam

AU - King,Emma M.

AU - DeLuca,Jennifer G.

AU - Swedlow,Jason R.

PY - 2010/10/4

Y1 - 2010/10/4

N2 - <p>We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions. Sds22 is required for proper progression through mitosis and localization of PP1 to mitotic kinetochores. Depletion of Sds22 increases aurora B T-loop phosphorylation and the rate of recovery from monastrol arrest. Phospho-aurora B accumulates at kinetochores in Sds22-depleted cells juxtaposed to critical kinetochore substrates. Sds22 modulates sister kinetochore distance and the interaction between Hec1 and the microtubule lattice and, thus, the activation of the spindle assembly checkpoint. These results demonstrate that Sds22 specifically defines PP1 function and localization in mitosis. Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface.</p>

AB - <p>We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions. Sds22 is required for proper progression through mitosis and localization of PP1 to mitotic kinetochores. Depletion of Sds22 increases aurora B T-loop phosphorylation and the rate of recovery from monastrol arrest. Phospho-aurora B accumulates at kinetochores in Sds22-depleted cells juxtaposed to critical kinetochore substrates. Sds22 modulates sister kinetochore distance and the interaction between Hec1 and the microtubule lattice and, thus, the activation of the spindle assembly checkpoint. These results demonstrate that Sds22 specifically defines PP1 function and localization in mitosis. Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface.</p>

KW - HISTONE H3 PHOSPHORYLATION

KW - STRUCTURED ILLUMINATION MICROSCOPY

KW - LIFETIME IMAGING MICROSCOPY

KW - PROTEIN PHOSPHATASE 1

KW - CHROMOSOME SEGREGATION

KW - ESSENTIAL ROLES

KW - CELL-DIVISION

KW - KINASE

KW - SPINDLE

KW - YEAST

U2 - 10.1083/jcb.200912046

DO - 10.1083/jcb.200912046

M1 - Article

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

VL - 191

SP - 61

EP - 74

ER -

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