Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. / Posch, Markus; Khoudoli, Guennadi A.; Swift, Sam; King, Emma M.; DeLuca, Jennifer G.; Swedlow, Jason R.
In: Journal of Cell Biology, Vol. 191, No. 1, 04.10.2010, p. 61-74.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis
A1 - Posch,Markus
A1 - Khoudoli,Guennadi A.
A1 - Swift,Sam
A1 - King,Emma M.
A1 - DeLuca,Jennifer G.
A1 - Swedlow,Jason R.
AU - Posch,Markus
AU - Khoudoli,Guennadi A.
AU - Swift,Sam
AU - King,Emma M.
AU - DeLuca,Jennifer G.
AU - Swedlow,Jason R.
PY - 2010/10/4
Y1 - 2010/10/4
N2 - <p>We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions. Sds22 is required for proper progression through mitosis and localization of PP1 to mitotic kinetochores. Depletion of Sds22 increases aurora B T-loop phosphorylation and the rate of recovery from monastrol arrest. Phospho-aurora B accumulates at kinetochores in Sds22-depleted cells juxtaposed to critical kinetochore substrates. Sds22 modulates sister kinetochore distance and the interaction between Hec1 and the microtubule lattice and, thus, the activation of the spindle assembly checkpoint. These results demonstrate that Sds22 specifically defines PP1 function and localization in mitosis. Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface.</p>
AB - <p>We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions. Sds22 is required for proper progression through mitosis and localization of PP1 to mitotic kinetochores. Depletion of Sds22 increases aurora B T-loop phosphorylation and the rate of recovery from monastrol arrest. Phospho-aurora B accumulates at kinetochores in Sds22-depleted cells juxtaposed to critical kinetochore substrates. Sds22 modulates sister kinetochore distance and the interaction between Hec1 and the microtubule lattice and, thus, the activation of the spindle assembly checkpoint. These results demonstrate that Sds22 specifically defines PP1 function and localization in mitosis. Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface.</p>
KW - HISTONE H3 PHOSPHORYLATION
KW - STRUCTURED ILLUMINATION MICROSCOPY
KW - LIFETIME IMAGING MICROSCOPY
KW - PROTEIN PHOSPHATASE 1
KW - CHROMOSOME SEGREGATION
KW - ESSENTIAL ROLES
KW - CELL-DIVISION
KW - KINASE
KW - SPINDLE
KW - YEAST
U2 - 10.1083/jcb.200912046
DO - 10.1083/jcb.200912046
M1 - Article
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 1
VL - 191
SP - 61
EP - 74
ER -