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Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni

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Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni. / Klaas, Mariliis; Oetke, Cornelia; Lewis, Leanne E; Erwig, Lars P; Heikema, Astrid P; Easton, Alistair; Willison, Hugh J; Crocker, Paul R (Lead / Corresponding author).

In: Journal of Immunology, Vol. 189, No. 5, 01.09.2012, p. 2414-22.

Research output: Contribution to journalArticle

Harvard

Klaas, M, Oetke, C, Lewis, LE, Erwig, LP, Heikema, AP, Easton, A, Willison, HJ & Crocker, PR 2012, 'Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni' Journal of Immunology, vol 189, no. 5, pp. 2414-22.

APA

Klaas, M., Oetke, C., Lewis, L. E., Erwig, L. P., Heikema, A. P., Easton, A., Willison, H. J., & Crocker, P. R. (2012). Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni. Journal of Immunology, 189(5), 2414-22doi: 10.4049/jimmunol.1200776

Vancouver

Klaas M, Oetke C, Lewis LE, Erwig LP, Heikema AP, Easton A et al. Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni. Journal of Immunology. 2012 Sep 1;189(5):2414-22.

Author

Klaas, Mariliis; Oetke, Cornelia; Lewis, Leanne E; Erwig, Lars P; Heikema, Astrid P; Easton, Alistair; Willison, Hugh J; Crocker, Paul R (Lead / Corresponding author) / Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni.

In: Journal of Immunology, Vol. 189, No. 5, 01.09.2012, p. 2414-22.

Research output: Contribution to journalArticle

Bibtex - Download

@article{6fbb1b5e3b6a472cad495f7ccca2ad71,
title = "Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni",
author = "Mariliis Klaas and Cornelia Oetke and Lewis, {Leanne E} and Erwig, {Lars P} and Heikema, {Astrid P} and Alistair Easton and Willison, {Hugh J} and Crocker, {Paul R}",
year = "2012",
volume = "189",
number = "5",
pages = "2414--22",
journal = "Journal of Immunology",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni

A1 - Klaas,Mariliis

A1 - Oetke,Cornelia

A1 - Lewis,Leanne E

A1 - Erwig,Lars P

A1 - Heikema,Astrid P

A1 - Easton,Alistair

A1 - Willison,Hugh J

A1 - Crocker,Paul R

AU - Klaas,Mariliis

AU - Oetke,Cornelia

AU - Lewis,Leanne E

AU - Erwig,Lars P

AU - Heikema,Astrid P

AU - Easton,Alistair

AU - Willison,Hugh J

AU - Crocker,Paul R

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Sialoadhesin (Sn) is a macrophage (M)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of Ms with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan-binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-a, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-a and IFN-ß responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with Ms in both liver and spleen. In the spleen, IFN-ß-reactive cells were localized to Sn(+) Ms and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.

AB - Sialoadhesin (Sn) is a macrophage (M)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of Ms with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan-binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-a, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-a and IFN-ß responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with Ms in both liver and spleen. In the spleen, IFN-ß-reactive cells were localized to Sn(+) Ms and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.

U2 - 10.4049/jimmunol.1200776

DO - 10.4049/jimmunol.1200776

M1 - Article

JO - Journal of Immunology

JF - Journal of Immunology

IS - 5

VL - 189

SP - 2414

EP - 2422

ER -

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