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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

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Authors

  • Yoshihiko Arita
  • Maria Leilani Torres-Mapa
  • Woei Ming Lee
  • Tomas Cizmar
  • Paul Campbell
  • Frank J. Gunn-Moore
  • Kishan Dholakia

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Original languageEnglish
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Number of pages3
JournalApplied Physics Letters
Journal publication date2 Mar 2011
Journal number9
Volume98
DOIs
StatePublished

Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

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