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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

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Original languageEnglish
Article number093702
Number of pages3
JournalApplied Physics Letters
Volume98
Issue number9
DOIs
StatePublished - 2 Mar 2011

Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

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