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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. / Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Cizmar, Tomas; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: Applied Physics Letters, Vol. 98, No. 9, 093702, 02.03.2011.

Research output: Contribution to journalArticle

Harvard

Arita, Y, Torres-Mapa, ML, Lee, WM, Cizmar, T, Campbell, P, Gunn-Moore, FJ & Dholakia, K 2011, 'Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles' Applied Physics Letters, vol 98, no. 9, 093702., 10.1063/1.3554415

APA

Arita, Y., Torres-Mapa, M. L., Lee, W. M., Cizmar, T., Campbell, P., Gunn-Moore, F. J., & Dholakia, K. (2011). Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. Applied Physics Letters, 98(9), [093702]. 10.1063/1.3554415

Vancouver

Arita Y, Torres-Mapa ML, Lee WM, Cizmar T, Campbell P, Gunn-Moore FJ et al. Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. Applied Physics Letters. 2011 Mar 2;98(9). 093702. Available from: 10.1063/1.3554415

Author

Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Cizmar, Tomas; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan / Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles.

In: Applied Physics Letters, Vol. 98, No. 9, 093702, 02.03.2011.

Research output: Contribution to journalArticle

Bibtex - Download

@article{5f68d3223ab542308032883878c87e21,
title = "Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles",
keywords = "INDUCED CELL-LYSIS, CAVITATION BUBBLES, SONOPORATION, GENERATION, COLLAPSE, WAVES",
author = "Yoshihiko Arita and Torres-Mapa, {Maria Leilani} and Lee, {Woei Ming} and Tomas Cizmar and Paul Campbell and Gunn-Moore, {Frank J.} and Kishan Dholakia",
year = "2011",
doi = "10.1063/1.3554415",
volume = "98",
number = "9",
journal = "Applied Physics Letters",
issn = "0003-6951",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

A1 - Arita,Yoshihiko

A1 - Torres-Mapa,Maria Leilani

A1 - Lee,Woei Ming

A1 - Cizmar,Tomas

A1 - Campbell,Paul

A1 - Gunn-Moore,Frank J.

A1 - Dholakia,Kishan

AU - Arita,Yoshihiko

AU - Torres-Mapa,Maria Leilani

AU - Lee,Woei Ming

AU - Cizmar,Tomas

AU - Campbell,Paul

AU - Gunn-Moore,Frank J.

AU - Dholakia,Kishan

PY - 2011/3/2

Y1 - 2011/3/2

N2 - <p>We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.</p>

AB - <p>We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.</p>

KW - INDUCED CELL-LYSIS

KW - CAVITATION BUBBLES

KW - SONOPORATION

KW - GENERATION

KW - COLLAPSE

KW - WAVES

U2 - 10.1063/1.3554415

DO - 10.1063/1.3554415

M1 - Article

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 9

VL - 98

ER -

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