Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles. / Arita, Yoshihiko; Torres-Mapa, Maria Leilani; Lee, Woei Ming; Cizmar, Tomas; Campbell, Paul; Gunn-Moore, Frank J.; Dholakia, Kishan.
In: Applied Physics Letters, Vol. 98, No. 9, 28.02.2011, p. -, 093702.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
A1 - Arita,Yoshihiko
A1 - Torres-Mapa,Maria Leilani
A1 - Lee,Woei Ming
A1 - Cizmar,Tomas
A1 - Campbell,Paul
A1 - Gunn-Moore,Frank J.
A1 - Dholakia,Kishan
AU - Arita,Yoshihiko
AU - Torres-Mapa,Maria Leilani
AU - Lee,Woei Ming
AU - Cizmar,Tomas
AU - Campbell,Paul
AU - Gunn-Moore,Frank J.
AU - Dholakia,Kishan
PY - 2011/2/28
Y1 - 2011/2/28
N2 - <p>We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. (C) 2011 American Institute of Physics. [doi:10.1063/1.3554415]</p>
AB - <p>We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. (C) 2011 American Institute of Physics. [doi:10.1063/1.3554415]</p>
KW - INDUCED CELL-LYSIS
KW - CAVITATION BUBBLES
KW - SONOPORATION
KW - GENERATION
KW - COLLAPSE
KW - WAVES
U2 - 10.1063/1.3554415
DO - 10.1063/1.3554415
M1 - Article
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 9
VL - 98
SP - -
ER -