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Stress induced gene expression

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Stress induced gene expression : a direct role for MAPKAP kinases in transcriptional activation of immediate early genes. / Ronkina, N.; Menon, M. B.; Schwermann, J.; Arthur, J. S. C.; Legault, H.; Telliez, J. -B.; Kayyali, U. S.; Nebreda, A. R.; Kotlyarov, A.; Gaestel, M.

In: Nucleic Acids Research, Vol. 39, No. 7, 04.2011, p. 2503-2518.

Research output: Contribution to journalArticle

Harvard

Ronkina, N, Menon, MB, Schwermann, J, Arthur, JSC, Legault, H, Telliez, J-B, Kayyali, US, Nebreda, AR, Kotlyarov, A & Gaestel, M 2011, 'Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes' Nucleic Acids Research, vol 39, no. 7, pp. 2503-2518., 10.1093/nar/gkq1178

APA

Ronkina, N., Menon, M. B., Schwermann, J., Arthur, J. S. C., Legault, H., Telliez, J. -B., ... Gaestel, M. (2011). Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes. Nucleic Acids Research, 39(7), 2503-2518. 10.1093/nar/gkq1178

Vancouver

Ronkina N, Menon MB, Schwermann J, Arthur JSC, Legault H, Telliez J-B et al. Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes. Nucleic Acids Research. 2011 Apr;39(7):2503-2518. Available from: 10.1093/nar/gkq1178

Author

Ronkina, N.; Menon, M. B.; Schwermann, J.; Arthur, J. S. C.; Legault, H.; Telliez, J. -B.; Kayyali, U. S.; Nebreda, A. R.; Kotlyarov, A.; Gaestel, M. / Stress induced gene expression : a direct role for MAPKAP kinases in transcriptional activation of immediate early genes.

In: Nucleic Acids Research, Vol. 39, No. 7, 04.2011, p. 2503-2518.

Research output: Contribution to journalArticle

Bibtex - Download

@article{738347e32d1b40da8972e61d451bd0a1,
title = "Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes",
author = "N. Ronkina and Menon, {M. B.} and J. Schwermann and Arthur, {J. S. C.} and H. Legault and Telliez, {J. -B.} and Kayyali, {U. S.} and Nebreda, {A. R.} and A. Kotlyarov and M. Gaestel",
year = "2011",
doi = "10.1093/nar/gkq1178",
volume = "39",
number = "7",
pages = "2503--2518",
journal = "Nucleic Acids Research",
issn = "0305-1048",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Stress induced gene expression

T2 - a direct role for MAPKAP kinases in transcriptional activation of immediate early genes

A1 - Ronkina,N.

A1 - Menon,M. B.

A1 - Schwermann,J.

A1 - Arthur,J. S. C.

A1 - Legault,H.

A1 - Telliez,J. -B.

A1 - Kayyali,U. S.

A1 - Nebreda,A. R.

A1 - Kotlyarov,A.

A1 - Gaestel,M.

AU - Ronkina,N.

AU - Menon,M. B.

AU - Schwermann,J.

AU - Arthur,J. S. C.

AU - Legault,H.

AU - Telliez,J. -B.

AU - Kayyali,U. S.

AU - Nebreda,A. R.

AU - Kotlyarov,A.

AU - Gaestel,M.

PY - 2011/4

Y1 - 2011/4

N2 - <p>Immediate early gene (IEG) expression is coordinated by multiple MAP kinase signaling pathways in a signal specific manner. Stress-activated p38 alpha MAP kinase is implicated in transcriptional regulation of IEGs via MSK-mediated CREB phosphorylation. The protein kinases downstream to p38, MAPKAP kinase (MK) 2 and MK3 have been identified to regulate gene expression at the posttranscriptional levels of mRNA stability and translation. Here, we analyzed stress-induced IEG expression in MK2/3-deficient cells. Ablation of MKs causes a decrease of p38 alpha level and p38-dependent IEG expression. Unexpectedly, restoration of p38 alpha does not rescue the full-range IEG response. Instead, the catalytic activity of MKs is necessary for the major transcriptional activation of IEGs. By transcriptomics, we identified MK2-regulated genes and recognized the serum response element (SRE) as a common promoter element. We show that stress-induced phosphorylation of serum response factor (SRF) at serine residue 103 is significantly reduced and that induction of SRE-dependent reporter activity is impaired and can only be rescued by catalytically active MK2 in MK2/3-deficient cells. Hence, a new function of MKs in transcriptional activation of IEGs via the p38 alpha-MK2/3-SRF-axis is proposed which probably cooperates with MKs' role in posttranscriptional gene expression in inflammation and stress response.</p>

AB - <p>Immediate early gene (IEG) expression is coordinated by multiple MAP kinase signaling pathways in a signal specific manner. Stress-activated p38 alpha MAP kinase is implicated in transcriptional regulation of IEGs via MSK-mediated CREB phosphorylation. The protein kinases downstream to p38, MAPKAP kinase (MK) 2 and MK3 have been identified to regulate gene expression at the posttranscriptional levels of mRNA stability and translation. Here, we analyzed stress-induced IEG expression in MK2/3-deficient cells. Ablation of MKs causes a decrease of p38 alpha level and p38-dependent IEG expression. Unexpectedly, restoration of p38 alpha does not rescue the full-range IEG response. Instead, the catalytic activity of MKs is necessary for the major transcriptional activation of IEGs. By transcriptomics, we identified MK2-regulated genes and recognized the serum response element (SRE) as a common promoter element. We show that stress-induced phosphorylation of serum response factor (SRF) at serine residue 103 is significantly reduced and that induction of SRE-dependent reporter activity is impaired and can only be rescued by catalytically active MK2 in MK2/3-deficient cells. Hence, a new function of MKs in transcriptional activation of IEGs via the p38 alpha-MK2/3-SRF-axis is proposed which probably cooperates with MKs' role in posttranscriptional gene expression in inflammation and stress response.</p>

U2 - 10.1093/nar/gkq1178

DO - 10.1093/nar/gkq1178

M1 - Article

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

VL - 39

SP - 2503

EP - 2518

ER -

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