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SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter

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SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter. / Gkikopoulos, Triantafyllos; Havas, Kristina M.; Dewar, Hilary; Owen-Hughes, Tom.

In: Molecular and Cellular Biology, Vol. 29, No. 15, 08.2009, p. 4057-4066.

Research output: Contribution to journalArticle

Harvard

Gkikopoulos, T, Havas, KM, Dewar, H & Owen-Hughes, T 2009, 'SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter' Molecular and Cellular Biology, vol 29, no. 15, pp. 4057-4066.

APA

Gkikopoulos, T., Havas, K. M., Dewar, H., & Owen-Hughes, T. (2009). SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter. Molecular and Cellular Biology, 29(15), 4057-4066doi: 10.1128/MCB.00400-09

Vancouver

Gkikopoulos T, Havas KM, Dewar H, Owen-Hughes T. SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter. Molecular and Cellular Biology. 2009 Aug;29(15):4057-4066.

Author

Gkikopoulos, Triantafyllos; Havas, Kristina M.; Dewar, Hilary; Owen-Hughes, Tom / SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter.

In: Molecular and Cellular Biology, Vol. 29, No. 15, 08.2009, p. 4057-4066.

Research output: Contribution to journalArticle

Bibtex - Download

@article{3d48c845a8bd41398ee41ae6d5f6e713,
title = "SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter",
author = "Triantafyllos Gkikopoulos and Havas, {Kristina M.} and Hilary Dewar and Tom Owen-Hughes",
year = "2009",
volume = "29",
number = "15",
pages = "4057--4066",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter

A1 - Gkikopoulos,Triantafyllos

A1 - Havas,Kristina M.

A1 - Dewar,Hilary

A1 - Owen-Hughes,Tom

AU - Gkikopoulos,Triantafyllos

AU - Havas,Kristina M.

AU - Dewar,Hilary

AU - Owen-Hughes,Tom

PY - 2009/8

Y1 - 2009/8

N2 - <p>Regulation of the Saccharomyces cerevisiae HO promoter has been shown to require the recruitment of chromatin-modifying and -remodeling enzymes. Despite this, relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here, we used indirect end labeling in synchronized cultures to show that the chromatin structure is disrupted in a region that spans bp -600 to -1800 relative to the transcriptional start site. Across this region, there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking, as monitored by chromatin immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF-related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.</p>

AB - <p>Regulation of the Saccharomyces cerevisiae HO promoter has been shown to require the recruitment of chromatin-modifying and -remodeling enzymes. Despite this, relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here, we used indirect end labeling in synchronized cultures to show that the chromatin structure is disrupted in a region that spans bp -600 to -1800 relative to the transcriptional start site. Across this region, there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking, as monitored by chromatin immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF-related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.</p>

KW - YEAST PHO5 PROMOTER

KW - IN-VIVO

KW - NUCLEOSOME OCCUPANCY

KW - CELL-CYCLE

KW - TRANSCRIPTIONAL ACTIVATION

KW - CHROMATIN-STRUCTURE

KW - CHAPERONE ASF1

KW - RNA-POLYMERASE

KW - DNA-BINDING

KW - SWI-SNF

U2 - 10.1128/MCB.00400-09

DO - 10.1128/MCB.00400-09

M1 - Article

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 15

VL - 29

SP - 4057

EP - 4066

ER -

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