Discovery - University of Dundee - Online Publications

Library & Learning Centre

Synthesis of novel tetrazole C5-linked C0- and C2-ribonucleoside phosphoramidites using MePOM and POM groups for probing RNA catalysis

Synthesis of novel tetrazole C5-linked C0- and C2-ribonucleoside phosphoramidites using MePOM and POM groups for probing RNA catalysis

Research output: Contribution to journalArticle

View graph of relations

Authors

  • Shinya Harusawa
  • Hiroki Yoneyama
  • Daiki Fujisue
  • Masayoshi Nishiura
  • Mihoyo Fujitake
  • Yoshihide Usami
  • Zheng-yun Zhao
  • Scott A. Mcphee
  • Timothy J. Wilson
  • David M.J. Lilley

Research units

Info

Original languageEnglish
Pages5891-5894
Number of pages4
JournalTetrahedron Letters
Journal publication date2012
Volume53
Issue44
DOIs
StatePublished

Abstract

Novel C5-linked C0- and C2-tetrazole ribonucleoside phosphoramidites were designed and synthesized via tetrazole C-nucleosides. Pivaloyloxymethyl (POM) and methyl-substituted POM (MePOM) groups were introduced as N-protecting groups in the tetrazole ring that can be readily removed under mild basic conditions. The phosphoramidites were successfully incorporated into the VS ribozyme substrate and hence providing a chemogenetic approach to determine which nucleobases of ribozymes function as the acid or base, in the studies of ribozyme general acid and base catalysis. © 2012 Elsevier Ltd. All rights reserved.

Documents

Library & Learning Centre

Contact | Accessibility | Policy