The application of LTR retrotransposons as molecular markers in plants. / Schulman, A.H.; Flavell, A.J.; Paux, E.; Ellis, T.H.N.
Mobile genetic elements: protocols and genomic applications. ed. / John M. Walker; Yves Bigot. Vol. 859 2nd. ed. New York : Springer, 2012. p. 115-153 (Methods in Molecular Biology).Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
}
TY - CHAP
T1 - The application of LTR retrotransposons as molecular markers in plants
A1 - Schulman,A.H.
A1 - Flavell,A.J.
A1 - Paux,E.
A1 - Ellis,T.H.N.
AU - Schulman,A.H.
AU - Flavell,A.J.
AU - Paux,E.
AU - Ellis,T.H.N.
PB - Springer
CY - New York
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction. © 2012 Springer Science+Business Media, LLC.
AB - Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction. © 2012 Springer Science+Business Media, LLC.
U2 - 10.1007/978-1-61779-603-6_7
DO - 10.1007/978-1-61779-603-6_7
M1 - Other chapter contribution
SN - 978-1-61779-602-9
VL - 859
BT - Mobile genetic elements
T2 - Mobile genetic elements
A2 - Bigot,Yves
ED - Bigot,Yves
T3 - Methods in Molecular Biology
T3 - en_GB
SP - 115
EP - 153
ER -