TY - JOUR T1 - The C134W (402 C > G) FOXL2 mutation is absent in ovarian gynandroblastoma T2 - insights into the genesis of an unusual tumour A1 - Oparka,Richard A1 - Cassidy,Andrew A1 - Reilly,Stephanie A1 - Stenhouse,Alasdair A1 - McCluggage,W. Glenn A1 - Herrington,C. Simon AU - Oparka,Richard AU - Cassidy,Andrew AU - Reilly,Stephanie AU - Stenhouse,Alasdair AU - McCluggage,W. Glenn AU - Herrington,C. Simon PY - 2012/4 Y1 - 2012/4 N2 -

Aims: Ovarian gynandroblastomas are rare tumours that, by definition, comprise a combinationof components resembling both female, typically granulosa cell tumour (GCT), and male, typically Sertoli or Sertoli/Leydig cell tumour (ST/SLT), sex cord/stromal differentiation. The histogenesis of these tumours is unknown and, in view of the very strong association between the C134W (402 C> G) FOXL2 mutation and adult- type GCT, we analysed a series of gynandroblastomas for this mutation.

Methods and results: Both components of each lesion were isolated by laser capture microdissection and the C134W (402 C> G) FOXL2 mutation was analysed by polymerase chain reaction sequencing. No mutation was identified in either the GCT or ST / SLT component of six cases, three of which contained adult- type GCT.

Conclusions: This suggests that, despite their similar morphological appearances, the GCT- like component of gynandroblastoma has a different molecular basis from conventional adult- type GCT. This finding underscores a more general principle that morphological similarity does not necessarily indicate molecular identity.

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Aims: Ovarian gynandroblastomas are rare tumours that, by definition, comprise a combinationof components resembling both female, typically granulosa cell tumour (GCT), and male, typically Sertoli or Sertoli/Leydig cell tumour (ST/SLT), sex cord/stromal differentiation. The histogenesis of these tumours is unknown and, in view of the very strong association between the C134W (402 C> G) FOXL2 mutation and adult- type GCT, we analysed a series of gynandroblastomas for this mutation.

Methods and results: Both components of each lesion were isolated by laser capture microdissection and the C134W (402 C> G) FOXL2 mutation was analysed by polymerase chain reaction sequencing. No mutation was identified in either the GCT or ST / SLT component of six cases, three of which contained adult- type GCT.

Conclusions: This suggests that, despite their similar morphological appearances, the GCT- like component of gynandroblastoma has a different molecular basis from conventional adult- type GCT. This finding underscores a more general principle that morphological similarity does not necessarily indicate molecular identity.

UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84858999473&md5=795b82ef33f11ffad396a3df663f036c U2 - 10.1111/j.1365-2559.2011.04148.x DO - 10.1111/j.1365-2559.2011.04148.x M1 - Article JO - Histopathology JF - Histopathology SN - 0309-0167 IS - 5 VL - 60 SP - 838 EP - 842 ER -