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The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1

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The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1. / Mocquet, Vincent; Neusiedler, Julia; Rende, Francesca; Cluet, David; Robin, Jean-Phillipe; Terme, Jean-Michel; Dodon, Madelaine Duc; Wittmann, Jurgen; Morris, Christelle; Le Hir, Herve; Ciminale, Vincenzo; Jalinot, Pierre.

In: Journal of Virology, Vol. 86, No. 14, 2012, p. 7530-7543.

Research output: Contribution to journalArticle

Harvard

Mocquet, V, Neusiedler, J, Rende, F, Cluet, D, Robin, J-P, Terme, J-M, Dodon, MD, Wittmann, J, Morris, C, Le Hir, H, Ciminale, V & Jalinot, P 2012, 'The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1' Journal of Virology, vol 86, no. 14, pp. 7530-7543., 10.1128/JVI.07021-11

APA

Mocquet, V., Neusiedler, J., Rende, F., Cluet, D., Robin, J-P., Terme, J-M., ... Jalinot, P. (2012). The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1. Journal of Virology, 86(14), 7530-7543. 10.1128/JVI.07021-11

Vancouver

Mocquet V, Neusiedler J, Rende F, Cluet D, Robin J-P, Terme J-M et al. The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1. Journal of Virology. 2012;86(14):7530-7543. Available from: 10.1128/JVI.07021-11

Author

Mocquet, Vincent; Neusiedler, Julia; Rende, Francesca; Cluet, David; Robin, Jean-Phillipe; Terme, Jean-Michel; Dodon, Madelaine Duc; Wittmann, Jurgen; Morris, Christelle; Le Hir, Herve; Ciminale, Vincenzo; Jalinot, Pierre / The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1.

In: Journal of Virology, Vol. 86, No. 14, 2012, p. 7530-7543.

Research output: Contribution to journalArticle

Bibtex - Download

@article{50067428c1ca42898eefc1366ca47221,
title = "The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1",
author = "Vincent Mocquet and Julia Neusiedler and Francesca Rende and David Cluet and Jean-Phillipe Robin and Jean-Michel Terme and Dodon, {Madelaine Duc} and Jurgen Wittmann and Christelle Morris and {Le Hir}, Herve and Vincenzo Ciminale and Pierre Jalinot",
note = "Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2012",
doi = "10.1128/JVI.07021-11",
volume = "86",
pages = "7530--7543",
journal = "Journal of Virology",
issn = "0022-538X",
number = "14",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1

AU - Mocquet,Vincent

AU - Neusiedler,Julia

AU - Rende,Francesca

AU - Cluet,David

AU - Robin,Jean-Phillipe

AU - Terme,Jean-Michel

AU - Dodon,Madelaine Duc

AU - Wittmann,Jurgen

AU - Morris,Christelle

AU - Le Hir,Herve

AU - Ciminale,Vincenzo

AU - Jalinot,Pierre

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012

Y1 - 2012

N2 - In this report, we analyzed whether the degradation of mRNAs by the nonsense-mediated mRNA decay (NMD) pathway was affected in human T-lymphotropic virus type 1 (HTLV-1)-infected cells. This pathway was indeed strongly inhibited in C91PL, HUT102, and MT2 cells, and such an effect was also observed by the sole expression of the Tax protein in Jurkat and HeLa cells. In line with this activity, Tax binds INT6/EIF3E (here called INT6), which is a subunit of the translation initiation factor eukaryotic initiation factor 3 (eIF3) required for efficient NMD, as well as the NMD core factor upstream frameshift protein 1 (UPF1). It was also observed that Tax expression alters the morphology of processing bodies (P-bodies), the cytoplasmic structures which concentrate RNA degradation factors. The presence of UPF1 in these subcellular compartments was increased by Tax, whereas that of INT6 was decreased. In line with these effects, the level of the phosphorylated form of UPF1 was increased in the presence of Tax. Analysis of several mutants of the viral protein showed that the interaction with INT6 is necessary forNMD inhibition. The alteration ofmRNAstability was observed to affect viral transcripts, such as that coding for the HTLV-1 basic leucine zipper factor (HBZ), and also several cellular mRNAs sensitive to theNMDpathway. Our data indicate that the effect of Tax on viral and cellular gene expression is not restricted to transcriptional control but can also involve posttranscriptional regulation. © 2012, American Society for Microbiology.

AB - In this report, we analyzed whether the degradation of mRNAs by the nonsense-mediated mRNA decay (NMD) pathway was affected in human T-lymphotropic virus type 1 (HTLV-1)-infected cells. This pathway was indeed strongly inhibited in C91PL, HUT102, and MT2 cells, and such an effect was also observed by the sole expression of the Tax protein in Jurkat and HeLa cells. In line with this activity, Tax binds INT6/EIF3E (here called INT6), which is a subunit of the translation initiation factor eukaryotic initiation factor 3 (eIF3) required for efficient NMD, as well as the NMD core factor upstream frameshift protein 1 (UPF1). It was also observed that Tax expression alters the morphology of processing bodies (P-bodies), the cytoplasmic structures which concentrate RNA degradation factors. The presence of UPF1 in these subcellular compartments was increased by Tax, whereas that of INT6 was decreased. In line with these effects, the level of the phosphorylated form of UPF1 was increased in the presence of Tax. Analysis of several mutants of the viral protein showed that the interaction with INT6 is necessary forNMD inhibition. The alteration ofmRNAstability was observed to affect viral transcripts, such as that coding for the HTLV-1 basic leucine zipper factor (HBZ), and also several cellular mRNAs sensitive to theNMDpathway. Our data indicate that the effect of Tax on viral and cellular gene expression is not restricted to transcriptional control but can also involve posttranscriptional regulation. © 2012, American Society for Microbiology.

UR - http://www.scopus.com/inward/record.url?scp=84863760251&partnerID=8YFLogxK

U2 - 10.1128/JVI.07021-11

DO - 10.1128/JVI.07021-11

M3 - Article

VL - 86

SP - 7530

EP - 7543

JO - Journal of Virology

T2 - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 14

ER -

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