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The interaction of four-way DNA junctions with resolving enzymes

The interaction of four-way DNA junctions with resolving enzymes

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Authors

  • David M. J. Lilley

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Info

Original languageEnglish
Pages399-403
Number of pages5
JournalBiochemical Society Transactions
Journal publication dateApr 2010
Volume38
DOIs
StatePublished

Abstract

Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.

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