The interaction of four-way DNA junctions with resolving enzymes

TY - JOUR

T1 - The interaction of four-way DNA junctions with resolving enzymes

A1 - Lilley,David M. J.

AU - Lilley,David M. J.

PY - 2010/4

Y1 - 2010/4

N2 - <p>Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.</p>

AB - <p>Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.</p>

KW - DNA repair

KW - genetic recombination

KW - Holiday junction

KW - molecular recognition

KW - nuclease

KW - T4 ENDONUCLEASE VII

KW - HOLLIDAY-JUNCTION

KW - GENETIC-RECOMBINATION

KW - T7 ENDONUCLEASE

KW - CRYSTAL-STRUCTURE

KW - MAMMALIAN-CELLS

KW - GENE-3 ENDONUCLEASE

KW - YEAST

KW - RESOLUTION

KW - REPAIR

U2 - 10.1042/BST0380399

DO - 10.1042/BST0380399

M1 - Article

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

VL - 38

SP - 399

EP - 403

ER -