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The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End

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The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End. / Maure, Jean-Francois; Komoto, Shinya; Oku, Yusuke; Mino, Akihisa; Pasqualato, Sebastiano; Natsume, Kayo; Clayton, Lesley; Musacchio, Andrea; Tanaka, Tomoyuki U. (Lead / Corresponding author).

In: Current Biology, Vol. 21, No. 3, 08.02.2011, p. 207-213.

Research output: Contribution to journalArticle

Harvard

Maure, J-F, Komoto, S, Oku, Y, Mino, A, Pasqualato, S, Natsume, K, Clayton, L, Musacchio, A & Tanaka, TU 2011, 'The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End' Current Biology, vol 21, no. 3, pp. 207-213., 10.1016/j.cub.2010.12.050

APA

Maure, J-F., Komoto, S., Oku, Y., Mino, A., Pasqualato, S., Natsume, K., ... Tanaka, T. U. (2011). The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End. Current Biology, 21(3), 207-213. 10.1016/j.cub.2010.12.050

Vancouver

Maure J-F, Komoto S, Oku Y, Mino A, Pasqualato S, Natsume K et al. The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End. Current Biology. 2011 Feb 8;21(3):207-213. Available from: 10.1016/j.cub.2010.12.050

Author

Maure, Jean-Francois; Komoto, Shinya; Oku, Yusuke; Mino, Akihisa; Pasqualato, Sebastiano; Natsume, Kayo; Clayton, Lesley; Musacchio, Andrea; Tanaka, Tomoyuki U. (Lead / Corresponding author) / The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End.

In: Current Biology, Vol. 21, No. 3, 08.02.2011, p. 207-213.

Research output: Contribution to journalArticle

Bibtex - Download

@article{ff73e2cf650f4410afd0afe7e9cbd610,
title = "The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End",
keywords = "PRECOCIOUS SEPARATION",
author = "Jean-Francois Maure and Shinya Komoto and Yusuke Oku and Akihisa Mino and Sebastiano Pasqualato and Kayo Natsume and Lesley Clayton and Andrea Musacchio and Tanaka, {Tomoyuki U.}",
year = "2011",
doi = "10.1016/j.cub.2010.12.050",
volume = "21",
number = "3",
pages = "207--213",
journal = "Current Biology",
issn = "0960-9822",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End

A1 - Maure,Jean-Francois

A1 - Komoto,Shinya

A1 - Oku,Yusuke

A1 - Mino,Akihisa

A1 - Pasqualato,Sebastiano

A1 - Natsume,Kayo

A1 - Clayton,Lesley

A1 - Musacchio,Andrea

A1 - Tanaka,Tomoyuki U.

AU - Maure,Jean-Francois

AU - Komoto,Shinya

AU - Oku,Yusuke

AU - Mino,Akihisa

AU - Pasqualato,Sebastiano

AU - Natsume,Kayo

AU - Clayton,Lesley

AU - Musacchio,Andrea

AU - Tanaka,Tomoyuki U.

PY - 2011/2/8

Y1 - 2011/2/8

N2 - <p>Proper chromosome segregation in mitosis relies on correct kinetochore-microtubule (KT-MT) interactions. The KT initially interacts with the lateral surface of a single MT (lateral attachment) extending from a spindle pole and is subsequently anchored at the plus end of the MT (end-on attachment) [1]. The conversion from lateral to end-on attachment is crucial because end-on attachment is more robust [2-4] and thought to be necessary to sustain KT-MT attachment when tension is applied across sister KTs upon their biorientation [1]. The mechanism for this conversion is still elusive. The Ndc80 complex is an essential component of the KT-MT interface [1, 5], and here we studied a role of the Ndc80 loop region, a distinct motif looping out from the coiled-coil shaft of the complex [6], in Saccharomyces cerevisiae. With deletions or mutations of the loop region, the lateral KT-MT attachment occurred normally; however, subsequent conversion to end-on attachment was defective, leading to failure in sister KT biorientation. The Ndc80 loop region was required for Ndc80-Dam1 interaction and KT loading of the Dam1 complex, which in turn supported KT tethering to the dynamic MT plus end [3, 7]. The Ndc80 loop region, therefore, has an important role in the conversion from lateral to end-on attachment, a crucial maturation step of KT-MT interaction.</p>

AB - <p>Proper chromosome segregation in mitosis relies on correct kinetochore-microtubule (KT-MT) interactions. The KT initially interacts with the lateral surface of a single MT (lateral attachment) extending from a spindle pole and is subsequently anchored at the plus end of the MT (end-on attachment) [1]. The conversion from lateral to end-on attachment is crucial because end-on attachment is more robust [2-4] and thought to be necessary to sustain KT-MT attachment when tension is applied across sister KTs upon their biorientation [1]. The mechanism for this conversion is still elusive. The Ndc80 complex is an essential component of the KT-MT interface [1, 5], and here we studied a role of the Ndc80 loop region, a distinct motif looping out from the coiled-coil shaft of the complex [6], in Saccharomyces cerevisiae. With deletions or mutations of the loop region, the lateral KT-MT attachment occurred normally; however, subsequent conversion to end-on attachment was defective, leading to failure in sister KT biorientation. The Ndc80 loop region was required for Ndc80-Dam1 interaction and KT loading of the Dam1 complex, which in turn supported KT tethering to the dynamic MT plus end [3, 7]. The Ndc80 loop region, therefore, has an important role in the conversion from lateral to end-on attachment, a crucial maturation step of KT-MT interaction.</p>

KW - PRECOCIOUS SEPARATION

UR - http://www.scopus.com/inward/record.url?scp=79551716041&partnerID=8YFLogxK

U2 - 10.1016/j.cub.2010.12.050

DO - 10.1016/j.cub.2010.12.050

M1 - Article

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 3

VL - 21

SP - 207

EP - 213

ER -

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