The nuclear ATPase/adenylate kinase hCINAP is recruited to perinucleolar caps generated upon RNA pol.II inhibition. / Malekkou, Anna; Lederer, Carsten W.; Lamond, Angus I.; Santama, Niovi.
In: FEBS Letters, Vol. 584, No. 22, 19.11.2010, p. 4559-4564.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The nuclear ATPase/adenylate kinase hCINAP is recruited to perinucleolar caps generated upon RNA pol.II inhibition
A1 - Malekkou,Anna
A1 - Lederer,Carsten W.
A1 - Lamond,Angus I.
A1 - Santama,Niovi
AU - Malekkou,Anna
AU - Lederer,Carsten W.
AU - Lamond,Angus I.
AU - Santama,Niovi
PY - 2010/11/19
Y1 - 2010/11/19
N2 - <p>hCINAP is an atypical nucleoplasmic enzyme, combining structural features of adenylate kinases and ATPases, which exhibits dual enzymatic activity. It interacts with the Cajal Body marker coilin and its level of expression and enzymatic activity influence Cajal Body numbers. Here we show that upon specific transcriptional inhibition of RNA pol.II, hCINAP segregates in perinuclear caps identified as Dark Nucleolar Caps (DNCs). These are distinct from perinucleolar caps where coilin and fibrillarin (both Cajal Body components) accumulate. In DNCs, hCINAP co-localizes with Paraspeckle Protein (PSP1) and also co-segregates with PSP1, and not coilin, in nuclear and nucleolar foci upon UV irradiation.</p><p>Structured summary:</p><p>MINT-8048545: hCINAP (uniprotkb:Q9Y3D8) and PSP1 (uniprotkb:Q8WXF1) colocalize (MI:0403) by fluorescence microscopy (MI: 0416) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.</p>
AB - <p>hCINAP is an atypical nucleoplasmic enzyme, combining structural features of adenylate kinases and ATPases, which exhibits dual enzymatic activity. It interacts with the Cajal Body marker coilin and its level of expression and enzymatic activity influence Cajal Body numbers. Here we show that upon specific transcriptional inhibition of RNA pol.II, hCINAP segregates in perinuclear caps identified as Dark Nucleolar Caps (DNCs). These are distinct from perinucleolar caps where coilin and fibrillarin (both Cajal Body components) accumulate. In DNCs, hCINAP co-localizes with Paraspeckle Protein (PSP1) and also co-segregates with PSP1, and not coilin, in nuclear and nucleolar foci upon UV irradiation.</p><p>Structured summary:</p><p>MINT-8048545: hCINAP (uniprotkb:Q9Y3D8) and PSP1 (uniprotkb:Q8WXF1) colocalize (MI:0403) by fluorescence microscopy (MI: 0416) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.</p>
KW - Nuclear organization
KW - Cajal Body
KW - Coilin
KW - Paraspeckle Protein 1
KW - Paraspeckle
KW - NUCLEOLAR CAPS
KW - COILED BODIES
KW - CAJAL BODIES
KW - BODY
KW - DIFFERENTIATION
KW - REORGANIZATION
KW - PARASPECKLES
KW - ASSOCIATION
U2 - 10.1016/j.febslet.2010.10.044
DO - 10.1016/j.febslet.2010.10.044
M1 - Article
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 22
VL - 584
SP - 4559
EP - 4564
ER -