Discovery - University of Dundee - Online Publications

Library & Learning Centre

The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh

The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh

Research output: Contribution to journalArticle

View graph of relations


Research units


Original languageEnglish
Number of pages9
JournalArchives of Microbiology
Journal publication dateDec 2010


Abh is a transition state regulator of Bacillus subtilis that controls biofilm formation and the production of several diverse antimicrobial compounds. Using a high-throughput non-biased technique, we show for the first time that Abh influences the sensitivity of B. subtilis to diverse antimicrobial compounds. Following up on these findings with a combination of classical genetics and antibiotic susceptibility assays, we demonstrate that Abh influences cellular processes such as the remodelling of the cell wall. We present data demonstrating that the extracytoplasmic function sigma factor sigma(X) controls resistance to beta-lactam antibiotics by activating abh transcription. Downstream from Abh, activation of slrR expression by Abh is responsible for controlling the sensitivity of B. subtilis to such antibiotics due to the role that SlrR plays in regulating autolysin biosynthesis. The abh mutant additionally exhibits increased resistance to aminoglycoside antimicrobials. We confirm that aminoglycoside killing of B. subtilis is likely to be caused by oxidative damage but rule out the possibility that the increased resistance of the abh mutant to aminoglycosides is due to a general increase in resistance to oxidative stress.


Library & Learning Centre

Contact | Accessibility | Policy