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The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh

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The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh. / Murray, Ewan J.; Stanley-Wall, Nicola R.

In: Archives of Microbiology, Vol. 192, No. 12, 12.2010, p. 1059-1067.

Research output: Contribution to journalArticle

Harvard

Murray, EJ & Stanley-Wall, NR 2010, 'The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh' Archives of Microbiology, vol 192, no. 12, pp. 1059-1067.

APA

Murray, E. J., & Stanley-Wall, N. R. (2010). The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh. Archives of Microbiology, 192(12), 1059-1067doi: 10.1007/s00203-010-0630-4

Vancouver

Murray EJ, Stanley-Wall NR. The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh. Archives of Microbiology. 2010 Dec;192(12):1059-1067.

Author

Murray, Ewan J.; Stanley-Wall, Nicola R. / The sensitivity of Bacillus subtilis to diverse antimicrobial compounds is influenced by Abh.

In: Archives of Microbiology, Vol. 192, No. 12, 12.2010, p. 1059-1067.

Research output: Contribution to journalArticle

Bibtex - Download

@article{28bb24b9ba934b27aa51ef7dc0d8b399,
title = "The sensitivity of <em>Bacillus subtilis</em> to diverse antimicrobial compounds is influenced by Abh",
author = "Murray, {Ewan J.} and Stanley-Wall, {Nicola R.}",
year = "2010",
volume = "192",
number = "12",
pages = "1059--1067",
journal = "Archives of Microbiology",
issn = "0302-8933",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The sensitivity of <em>Bacillus subtilis</em> to diverse antimicrobial compounds is influenced by Abh

A1 - Murray,Ewan J.

A1 - Stanley-Wall,Nicola R.

AU - Murray,Ewan J.

AU - Stanley-Wall,Nicola R.

PY - 2010/12

Y1 - 2010/12

N2 - <p>Abh is a transition state regulator of Bacillus subtilis that controls biofilm formation and the production of several diverse antimicrobial compounds. Using a high-throughput non-biased technique, we show for the first time that Abh influences the sensitivity of B. subtilis to diverse antimicrobial compounds. Following up on these findings with a combination of classical genetics and antibiotic susceptibility assays, we demonstrate that Abh influences cellular processes such as the remodelling of the cell wall. We present data demonstrating that the extracytoplasmic function sigma factor sigma(X) controls resistance to beta-lactam antibiotics by activating abh transcription. Downstream from Abh, activation of slrR expression by Abh is responsible for controlling the sensitivity of B. subtilis to such antibiotics due to the role that SlrR plays in regulating autolysin biosynthesis. The abh mutant additionally exhibits increased resistance to aminoglycoside antimicrobials. We confirm that aminoglycoside killing of B. subtilis is likely to be caused by oxidative damage but rule out the possibility that the increased resistance of the abh mutant to aminoglycosides is due to a general increase in resistance to oxidative stress.</p>

AB - <p>Abh is a transition state regulator of Bacillus subtilis that controls biofilm formation and the production of several diverse antimicrobial compounds. Using a high-throughput non-biased technique, we show for the first time that Abh influences the sensitivity of B. subtilis to diverse antimicrobial compounds. Following up on these findings with a combination of classical genetics and antibiotic susceptibility assays, we demonstrate that Abh influences cellular processes such as the remodelling of the cell wall. We present data demonstrating that the extracytoplasmic function sigma factor sigma(X) controls resistance to beta-lactam antibiotics by activating abh transcription. Downstream from Abh, activation of slrR expression by Abh is responsible for controlling the sensitivity of B. subtilis to such antibiotics due to the role that SlrR plays in regulating autolysin biosynthesis. The abh mutant additionally exhibits increased resistance to aminoglycoside antimicrobials. We confirm that aminoglycoside killing of B. subtilis is likely to be caused by oxidative damage but rule out the possibility that the increased resistance of the abh mutant to aminoglycosides is due to a general increase in resistance to oxidative stress.</p>

KW - Bacillus subtilis

KW - Antibiotic sensitivity

KW - Abh

KW - AbrB

KW - FUNCTION SIGMA-FACTORS

KW - POPULATION HETEROGENEITY

KW - BIOFILM FORMATION

KW - CELL-SEPARATION

KW - ABRB GENE

KW - REGULATOR

KW - PROMOTER

KW - REGULON

KW - PROTEIN

KW - IDENTIFICATION

U2 - 10.1007/s00203-010-0630-4

DO - 10.1007/s00203-010-0630-4

M1 - Article

JO - Archives of Microbiology

JF - Archives of Microbiology

SN - 0302-8933

IS - 12

VL - 192

SP - 1059

EP - 1067

ER -

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