Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
| Original language | English |
|---|---|
| Title | Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B |
| Editors | Daniel Herschlag |
| Place of publication | San Diego |
| Publisher | Academic Press |
| Publication date | 2009 |
| Pages | 159-187 |
| Number of pages | 29 |
| Volume | 469 |
| ISBN (Print) | 9780123809223 |
| DOIs | |
| State | Published |
Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.