Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
|Title of host publication||Biophysical, chemical, and functional probes of RNA structure, interactions and folding|
|Subtitle of host publication||Part B|
|Place of publication||San Diego|
|Number of pages||29|
|Name||Methods in Enzymology|
Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.