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The Structure and Folding of Branched RNA Analyzed by Fluorescence Resonance Energy Transfer

The Structure and Folding of Branched RNA Analyzed by Fluorescence Resonance Energy Transfer

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Authors

  • David M. J. Lilley

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Info

Original languageEnglish
TitleMethods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
EditorsDaniel Herschlag
Place of publicationSan Diego
PublisherAcademic Press
Publication date2009
Pages159-187
Number of pages29
Volume469
ISBN (Print)9780123809223
DOIs
StatePublished

Abstract

Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.

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