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The structure and folding of branched RNA analyzed by fluorescence resonance energy transfer

The structure and folding of branched RNA analyzed by fluorescence resonance energy transfer

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Authors

  • David M. J. Lilley

Research units

Info

Original languageEnglish
Title of host publicationBiophysical, chemical, and functional probes of RNA structure, interactions and folding
Subtitle of host publicationPart B
EditorsDaniel Herschlag
Place of publicationSan Diego
PublisherAcademic Press
Publication date2009
Pages159-187
Number of pages29
ISBN (Print)9780123809223
DOIs
StatePublished

Publication series

NameMethods in Enzymology
PublisherAcademic Press
Volume469

Abstract

Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.

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