The Structure and Folding of Branched RNA Analyzed by Fluorescence Resonance Energy Transfer. / Lilley, David M. J.
Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B. ed. / Daniel Herschlag. Vol. 469 San Diego : Academic Press, 2009. p. 159-187.Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution
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TY - CHAP
T1 - The Structure and Folding of Branched RNA Analyzed by Fluorescence Resonance Energy Transfer
A1 - Lilley,David M. J.
AU - Lilley,David M. J.
PB - Academic Press
CY - San Diego
PY - 2009
Y1 - 2009
N2 - <p>Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.</p>
AB - <p>Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.</p>
KW - SINGLE-MOLECULE FRET
KW - DOUBLE-STRANDED DNA
KW - KINK-TURN MOTIF
KW - HAIRPIN RIBOZYME
KW - HAMMERHEAD RIBOZYME
KW - TERTIARY STRUCTURE
KW - SECONDARY STRUCTURE
KW - GLOBAL STRUCTURE
KW - 4-WAY JUNCTION
KW - NUCLEIC-ACIDS
U2 - 10.1016/S0076-6879(09)69008-X
DO - 10.1016/S0076-6879(09)69008-X
M1 - Other chapter contribution
SN - 9780123809223
VL - 469
BT - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
T2 - Methods in Enzymology: Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
A2 - Herschlag,Daniel
ED - Herschlag,Daniel
SP - 159
EP - 187
ER -