The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. / Gkikopoulos, Triantafyllos; Singh, Vijender; Tsui, Kyle; Awad, Salma; Renshaw, Matthew J.; Scholfield, Pieta; Barton, Geoffrey J.; Nislow, Corey; Tanaka, Tomoyuki U.; Owen-Hughes, Tom.
In: EMBO Journal, Vol. 30, No. 10, 18.05.2011, p. 1919-1927.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4
A1 - Gkikopoulos,Triantafyllos
A1 - Singh,Vijender
A1 - Tsui,Kyle
A1 - Awad,Salma
A1 - Renshaw,Matthew J.
A1 - Scholfield,Pieta
A1 - Barton,Geoffrey J.
A1 - Nislow,Corey
A1 - Tanaka,Tomoyuki U.
A1 - Owen-Hughes,Tom
AU - Gkikopoulos,Triantafyllos
AU - Singh,Vijender
AU - Tsui,Kyle
AU - Awad,Salma
AU - Renshaw,Matthew J.
AU - Scholfield,Pieta
AU - Barton,Geoffrey J.
AU - Nislow,Corey
AU - Tanaka,Tomoyuki U.
AU - Owen-Hughes,Tom
PY - 2011/5/18
Y1 - 2011/5/18
N2 - <p>In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011</p>
AB - <p>In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011</p>
KW - centromere
KW - chromatin
KW - Cse4
KW - nucleosome
KW - SWI/SNF
KW - CHROMATIN-REMODELING COMPLEX
KW - E3 UBIQUITIN LIGASE
KW - SACCHAROMYCES-CEREVISIAE
KW - BUDDING YEAST
KW - H3 VARIANT
KW - TRANSCRIPTIONAL ACTIVATION
KW - SPINDLE MICROTUBULES
KW - NUCLEOSOME OCCUPANCY
KW - MITOTIC SPINDLE
KW - PROTEIN COMPLEX
U2 - 10.1038/emboj.2011.112
DO - 10.1038/emboj.2011.112
M1 - Article
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 10
VL - 30
SP - 1919
EP - 1927
ER -