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Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates

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Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates. / Rakitina, D. V.; Taliansky, Michael; Brown, J. W. S.; Kalinina, N. O.

In: Nucleic Acids Research, Vol. 39, No. 20, 11.2011, p. 8869-8880.

Research output: Contribution to journalArticle

Harvard

Rakitina, DV, Taliansky, M, Brown, JWS & Kalinina, NO 2011, 'Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates' Nucleic Acids Research, vol 39, no. 20, pp. 8869-8880.

APA

Rakitina, D. V., Taliansky, M., Brown, J. W. S., & Kalinina, N. O. (2011). Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates. Nucleic Acids Research, 39(20), 8869-8880doi: 10.1093/nar/gkr594

Vancouver

Rakitina DV, Taliansky M, Brown JWS, Kalinina NO. Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates. Nucleic Acids Research. 2011 Nov;39(20):8869-8880.

Author

Rakitina, D. V.; Taliansky, Michael; Brown, J. W. S.; Kalinina, N. O. / Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates.

In: Nucleic Acids Research, Vol. 39, No. 20, 11.2011, p. 8869-8880.

Research output: Contribution to journalArticle

Bibtex - Download

@article{eca70dd4a5224c22b40d18437c307c00,
title = "Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates",
author = "Rakitina, {D. V.} and Michael Taliansky and Brown, {J. W. S.} and Kalinina, {N. O.}",
year = "2011",
volume = "39",
number = "20",
pages = "8869--8880",
journal = "Nucleic Acids Research",
issn = "0305-1048",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates

A1 - Rakitina,D. V.

A1 - Taliansky,Michael

A1 - Brown,J. W. S.

A1 - Kalinina,N. O.

AU - Rakitina,D. V.

AU - Taliansky,Michael

AU - Brown,J. W. S.

AU - Kalinina,N. O.

PY - 2011/11

Y1 - 2011/11

N2 - Fibrillarin, one of the major proteins of the nucleolus, plays several essential roles in ribosome biogenesis including pre-rRNA processing and 2'-O-ribose methylation of rRNA and snRNAs. Recently, it has been shown that fibrillarin plays a role in virus infections and is associated with viral RNPs. Here, we demonstrate the ability of recombinant fibrillarin 2 from Arabidopsis thaliana (AtFib2) to interact with RNAs of different lengths and types including rRNA, snoRNA, snRNA, siRNA and viral RNAs in vitro. Our data also indicate that AtFib2 possesses two RNA-binding sites in the central (138–179 amino acids) and C-terminal (225–281 amino acids) parts of the protein, respectively. The conserved GCVYAVEF octamer does not bind RNA directly as suggested earlier, but may assist with the proper folding of the central RNA-binding site.

AB - Fibrillarin, one of the major proteins of the nucleolus, plays several essential roles in ribosome biogenesis including pre-rRNA processing and 2'-O-ribose methylation of rRNA and snRNAs. Recently, it has been shown that fibrillarin plays a role in virus infections and is associated with viral RNPs. Here, we demonstrate the ability of recombinant fibrillarin 2 from Arabidopsis thaliana (AtFib2) to interact with RNAs of different lengths and types including rRNA, snoRNA, snRNA, siRNA and viral RNAs in vitro. Our data also indicate that AtFib2 possesses two RNA-binding sites in the central (138–179 amino acids) and C-terminal (225–281 amino acids) parts of the protein, respectively. The conserved GCVYAVEF octamer does not bind RNA directly as suggested earlier, but may assist with the proper folding of the central RNA-binding site.

U2 - 10.1093/nar/gkr594

DO - 10.1093/nar/gkr594

M1 - Article

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 20

VL - 39

SP - 8869

EP - 8880

ER -

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