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σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh

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σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh. / Murray, Ewan J.; Strauch, Mark A.; Stanley-Wall, Nicola R. (Lead / Corresponding author).

In: Journal of Bacteriology, Vol. 191, No. 22, 15.11.2009, p. 6822-6832.

Research output: Contribution to journalArticle

Harvard

Murray, EJ, Strauch, MA & Stanley-Wall, NR 2009, 'σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh' Journal of Bacteriology, vol 191, no. 22, pp. 6822-6832., 10.1128/JB.00618-09

APA

Murray, E. J., Strauch, M. A., & Stanley-Wall, N. R. (2009). σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh. Journal of Bacteriology, 191(22), 6822-6832. 10.1128/JB.00618-09

Vancouver

Murray EJ, Strauch MA, Stanley-Wall NR. σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh. Journal of Bacteriology. 2009 Nov 15;191(22):6822-6832. Available from: 10.1128/JB.00618-09

Author

Murray, Ewan J.; Strauch, Mark A.; Stanley-Wall, Nicola R. (Lead / Corresponding author) / σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh.

In: Journal of Bacteriology, Vol. 191, No. 22, 15.11.2009, p. 6822-6832.

Research output: Contribution to journalArticle

Bibtex - Download

@article{edc1939b1cc54d3794383e5b700573ef,
title = "σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh",
keywords = "TRANSITION-STATE REGULATOR, INTRAMEMBRANE PROTEOLYSIS, MULTICELLULAR BEHAVIOR, GENETIC COMPETENCE, SPORULATION GENE, STATIONARY-PHASE, STRESS RESPONSES, MASTER REGULATOR, PROTEIN, EXPRESSION",
author = "Murray, {Ewan J.} and Strauch, {Mark A.} and Stanley-Wall, {Nicola R.}",
year = "2009",
doi = "10.1128/JB.00618-09",
volume = "191",
number = "22",
pages = "6822--6832",
journal = "Journal of Bacteriology",
issn = "0021-9193",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - σ<sup>X </sup>Is Involved in Controlling <em>Bacillus subtilis </em>Biofilm Architecture through the AbrB Homologue Abh

A1 - Murray,Ewan J.

A1 - Strauch,Mark A.

A1 - Stanley-Wall,Nicola R.

AU - Murray,Ewan J.

AU - Strauch,Mark A.

AU - Stanley-Wall,Nicola R.

PY - 2009/11/15

Y1 - 2009/11/15

N2 - <p>A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription from these operons is repressed by two key regulators, AbrB and SinR. Relief of inhibition is necessary to allow biofilm formation to proceed. Here we present data indicating that Abh, a sequence and structural homologue of AbrB, regulates biofilm architecture by B. subtilis when colony morphology and pellicle formation are assessed. Data indicating that abh expression is dependent on the environmental signals that stimulate the activity of the extracytoplasmic function sigma-factor sigma(X) are shown. We demonstrate that expression of slrR, the proposed activator of yqxM transcription, is positively controlled by Abh. Furthermore, Abh is shown to activate transcription from the promoter of the eps operon through its control of SlrR. These findings add to the increasingly complex transcriptional network that controls biofilm formation by B. subtilis.</p>

AB - <p>A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription from these operons is repressed by two key regulators, AbrB and SinR. Relief of inhibition is necessary to allow biofilm formation to proceed. Here we present data indicating that Abh, a sequence and structural homologue of AbrB, regulates biofilm architecture by B. subtilis when colony morphology and pellicle formation are assessed. Data indicating that abh expression is dependent on the environmental signals that stimulate the activity of the extracytoplasmic function sigma-factor sigma(X) are shown. We demonstrate that expression of slrR, the proposed activator of yqxM transcription, is positively controlled by Abh. Furthermore, Abh is shown to activate transcription from the promoter of the eps operon through its control of SlrR. These findings add to the increasingly complex transcriptional network that controls biofilm formation by B. subtilis.</p>

KW - TRANSITION-STATE REGULATOR

KW - INTRAMEMBRANE PROTEOLYSIS

KW - MULTICELLULAR BEHAVIOR

KW - GENETIC COMPETENCE

KW - SPORULATION GENE

KW - STATIONARY-PHASE

KW - STRESS RESPONSES

KW - MASTER REGULATOR

KW - PROTEIN

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=70350443407&partnerID=8YFLogxK

U2 - 10.1128/JB.00618-09

DO - 10.1128/JB.00618-09

M1 - Article

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 22

VL - 191

SP - 6822

EP - 6832

ER -

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