X-ray absorption spectroscopy (XAS) of toxic metal mineral transformations by fungi. / Fomina, Marina; Charnock, John; Bowen, Andrew D.; Gadd, Geoffrey M.
In: Environmental Microbiology, Vol. 9, No. 2, 2007, p. 308-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - X-ray absorption spectroscopy (XAS) of toxic metal mineral transformations by fungi
A1 - Fomina,Marina
A1 - Charnock,John
A1 - Bowen,Andrew D.
A1 - Gadd,Geoffrey M.
AU - Fomina,Marina
AU - Charnock,John
AU - Bowen,Andrew D.
AU - Gadd,Geoffrey M.
PY - 2007
Y1 - 2007
N2 - Fungi can be highly efficient biogeochemical agents and accumulators of soluble and particulate forms of metals. This work aims to understand some of the physico-chemical mechanisms involved in toxic metal transformations focusing on the speciation of metals accumulated by fungi and mycorrhizal associations. The amorphous state or poor crystallinity of metal complexes within biomass and relatively low metal concentrations make the determination of metal speciation in biological systems a challenging problem but this can be overcome by using synchrotron-based element-specific X-ray absorption spectroscopy (XAS) techniques. In this research, we have exposed fungi and ectomycorrhizas to a variety of copper-, zinc- and lead-containing minerals. X-ray absorption spectroscopy studies revealed that oxygen ligands (phosphate, carboxylate) played a major role in toxic metal coordination within the fungal and ectomycorrhizal biomass during the accumulation of mobilized toxic metals. Coordination of toxic metals within biomass depended on the fungal species, initial mineral composition, the nitrogen source, and the physiological state/age of the fungal mycelium.
AB - Fungi can be highly efficient biogeochemical agents and accumulators of soluble and particulate forms of metals. This work aims to understand some of the physico-chemical mechanisms involved in toxic metal transformations focusing on the speciation of metals accumulated by fungi and mycorrhizal associations. The amorphous state or poor crystallinity of metal complexes within biomass and relatively low metal concentrations make the determination of metal speciation in biological systems a challenging problem but this can be overcome by using synchrotron-based element-specific X-ray absorption spectroscopy (XAS) techniques. In this research, we have exposed fungi and ectomycorrhizas to a variety of copper-, zinc- and lead-containing minerals. X-ray absorption spectroscopy studies revealed that oxygen ligands (phosphate, carboxylate) played a major role in toxic metal coordination within the fungal and ectomycorrhizal biomass during the accumulation of mobilized toxic metals. Coordination of toxic metals within biomass depended on the fungal species, initial mineral composition, the nitrogen source, and the physiological state/age of the fungal mycelium.
KW - X-ray absorption spectroscopy (XAS)
KW - Toxic metal
KW - Physico-chemical mechanisms
U2 - 10.1111/j.1462-2920.2006.01139.x
DO - 10.1111/j.1462-2920.2006.01139.x
M1 - Article
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 2
VL - 9
SP - 308
EP - 321
ER -