Microplate-Based Quantification of Poly-γ-Glutamic Acid (PGA) in Bacillus subtilis Biofilm Samples

This dataset contains the raw and processed absorbance data, standard curve measurements, and associated metadata supporting the development of a microplate-based methylene blue assay for quantifying poly-γ-glutamic acid (PGA) in Bacillus subtilis biofilm extracts.

The dataset includes:

· Raw absorbance spectra (400–800 nm) from microplate reader experiments

· Absorbance values at 564 nm and 664 nm used for PGA quantification

· Independent PGA standard curve replicates

· Background-corrected datasets

· Protein quantification (BCA assay) data used for normalisation

· Replicate information and regression equations used for concentration calculations

These data support the conclusions presented in the associated publication and enable reproducibility of the quantification method.

Contributors and Roles

David Stevenson (DS): Conceptualisation; Methodology; Validation; Formal analysis; Investigation; Resources; Data curation; Visualisation; Writing – original draft; Project administration.

Nicola R. Stanley-Wall (NSW): Formal analysis; Resources; Data curation; Visualization; Writing – original draft; Writing – review & editing; Supervision; Project administration; Funding acquisition.

C. E. MacPhee (CEM): Writing – original draft; Writing – review & editing; Supervision; Project administration; Funding acquisition.

Philippa Sterlini (PS) – Data deposition