Proteomic analysis of FPA interactors

  • Matthew Parker (Creator)
  • Katarzyna Knop (Creator)
  • Vasiliki Zacharaki (Creator)
  • Anya Sherwood (Creator)
  • D. Tome (Creator)
  • X. Yu (Creator)
  • P. Martin (Creator)
  • J. Beynon (Creator)
  • S. Michaels (Creator)
  • Geoffrey Barton (Creator)
  • Gordon Simpson (Creator)



Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat receptor genes (NLRs) are found in plant genomes and provide disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, controlling their functional expression and impacting immunity. Using long-read nanopore direct RNA sequencing we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in immunity.
Date made available16 Dec 2020
PublisherPRoteomics IDEntifications Database

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