Reconstruction of distinct vertebrate gastrulation modes via modulation of key cell behaviors in the chick embryo

    Dataset

    Description

    Movie S1. Development of control embryo from stage HH1 to HH3+. The movie shows the development of a control embryo (left panel) and a zoom-in of the formation of the primitive streak (right panel). Inset side 650 µm. The time interval is 3 minutes.,

    Movie S2. Strain rates and deformation grid of control embryo from stage HH1 to HH3+. The movie shows a bright field image (top panel) and strain rate tensor of the same embryo (bottom panel) Isotropic strain rate is colored blue (contraction) to red (expansion) scale bar 500 µm. The time interval is 3 minutes.

    Movie S3. Development of embryo treated with FGF signaling inhibitor LY287455. The movie shows a bright field image (top panel) and strain rate tensor of the same embryo (bottom panel). Isotropic strain rate is colored blue (contraction) to red (expansion) scale bar 500 µm. The time interval is 3 minutes.

    Movie S4. Formation of an ectopic circular primitive streak in FGF2 treated embryo. The movie shows the development of an FGF2-treated embryo (left panel) and a zoom-in of the formation of the ectopic circular primitive streak (right panel). Inset side 650 µm. The time interval is 3 minutes.

    Movie S5. Strain rates and deformation grid of embryo treated with FGF2. The movie shows a bright field image (top panel) and strain rate tensor of the same embryo (bottom panel) Isotropic strain rate colored blue (contraction) to red (expansion) scale bar 500 µm. The time interval is 3 minutes.

    Movie S6. Migration of mesoderm cells after addition of FGF2. Focusing deeper into the embryo shows that the mesoderm cells ingressing through the circular primitive streak migrate towards the center of the embryo. The time interval is 3 minutes.

    Movie S7. Formation of large invagination of the central epiblast in embryos treated with CHIR+LDN. The movie shows the development of a CHIR + LDN treated embryo (left panel) and a zoom-in of the formation of the large invagination in the central epiblast (right panel). Inset side 650 µm. The time interval is 3 minutes.

    Movie S8. Strain rates and deformation grid of embryo treated with CHIR+LDN. The movie shows a bright field image (top panel) and strain rate tensor of the same embryo (bottom panel) Isotropic strain rate colored blue (contraction) to red (expansion) scale bar 500 µm. The time interval is 3 minutes.

    Movie S9. Formation of an invaginating lip in an embryo treated with Axitinib. The movie shows the development of an Axitinib-treated embryo (left panel) and a zoom-in of the formation of the invaginating lip (right panel). Inset side 650 µm. The time interval is 3 minutes.

    Movie S10. Strain rates and deformation grid of embryo treated with Axitinib. The movie shows a bright field image (left panel) and strain rate tensor of the same embryo (right panel) Isotropic strain rate colored blue (contraction) to red (expansion) scale bar 500 µm. The time interval is 3 minutes.

    Movie S11. Comparison of the tip of streak formation in control embryo and embryo treated with 100 nM Axitinib. The time interval is 3 minutes.
    Date made available18 Nov 2022
    PublisherUniversity of Dundee

    Data Monitor categories

    • cell behaviours,
    • gastrulation
    • light-sheet
    • tissue flows
    • morphogenesis

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