Streptococcus pneumoniae Genome sequencing

In this study we carried out manual passage of pneumococci between mice in a model of asymptomatic nasopharyngeal carriage and in parallel we also performed the same process in a pneumonia model of infection. The sequence reads from the evolved lineages were compared to a complete whole genome of the ancestor strain used for the initial infection inoculum. Using this approach we aimed to determine how environmental differences between the upper and lower airways might shape the adaptation and evolution processes of the pneumococcus.