School of Life Sciences

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Research Output 1968 2020

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Chapter (peer-reviewed)
2019

A Practical Guide to Barley Crossing

Thomas, W. T. B., Bull, H., Booth, A., Hamilton, R., Forster, B. P. & Franckowiak, J. D., 1 Jan 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 21-36 16 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hordeum
Inbreeding
Pollen
Seeds

Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods

Hurst, C. H., Turnbull, D. & Hemsley, P. A., 2019, Protein Lipidation: Methods and Protocols. Linder, M. E. (ed.). New York: Springer , Vol. 2009. p. 3-11 9 p. (Methods in Molecular Biology; vol. 2009).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Acylation
Protein S
Proteins
Post Translational Protein Processing
Cysteine

DiCre-Based Inducible Disruption of Leishmania Genes

Duncan, S. M., Myburgh, E., Alves-Ferreira, E. V. & Mottram, J. C., 2019, Leishmania: Methods and Protocols. Clos, J. (ed.). New York: Humana Press, Vol. 1971. p. 211-224 14 p. (Methods in Molecular Biology; vol. 1971).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leishmania
Essential Genes
Gene Deletion
Genes
Enzyme Activation

Exome Capture for Variant Discovery and Analysis in Barley

Bayer, M., Morris, J. A., Booth, C., Booth, A., Uzrek, N., Russell, J. R., Waugh, R. & Hedley, P. E., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 283-310 28 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Exome
Hordeum
Genome
Firearms
Computational Biology

High-Resolution RT-PCR Analysis of Alternative Barley Transcripts

Simpson, C. G., Fuller, J., Rapazote-Flores, P., Mayer, C. D., Calixto, C. P. G., Milne, L., Hedley, P. E., Booth, C., Waugh, R. & Brown, J. W. S., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 269-281 13 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hordeum
RNA
Polymerase Chain Reaction
Protein Isoforms
Genome
2 Citations (Scopus)

Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

McWilliams, T. G. & Ganley, I. G., 2019, Autophagy: Methods and Protocols. Ktistakis, N. & Florey, O. (eds.). New York: Humana Press, Vol. 1880. p. 621-642 22 p. (Methods in Molecular Biology; vol. 1880).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Mitochondrial Degradation
Quality Control
Autophagy
Organelles
Homeostasis

Methods to analyze STUbL activity

Branigan, E., Plechanovová, A. & Hay, R. T., 3 Jan 2019, Methods in Enzymology. Elsevier, Vol. 618. p. 257-280 24 p. (Methods in Enzymology; vol. 618).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Ubiquitin-Protein Ligases
Ubiquitin
Genes
Assays
Substrates
1 Citation (Scopus)

Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

Mittmann, S., Arrieta, M., Ramsay, L., Waugh, R. & Colas, I., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 167-179 13 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hordeum
Pollen
Microscopy
Stem Cells
Genetic Recombination
2018
1 Citation (Scopus)

Biochemical and Structural Properties of Fungal Holliday Junction-Resolving Enzymes

Liu, Y., Freeman, A., Déclais, A-C., Gartner, A. & Lilley, D. M. J., 2018, Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination. Spies, M. & Malkova, A. (eds.). Academic Press, Vol. 600. p. 543-568 26 p. (Methods in Enzymology; vol. 600).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Cruciform DNA
Structural properties
Enzymes
Chaetomium
DNA
1 Citation (Scopus)

Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK

Fyffe, F. A., Hawley, S. A., Gray, A. & Hardie, D. G., 2018, AMPK : Methods and Protocols. Neumann, D. & Viollet, B. (eds.). New York: Humana Press, p. 69-86 18 p. (Methods in Molecular Biology; vol. 1732).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

AMP-Activated Protein Kinases
Adenosine Triphosphate
Adenosine Monophosphate
Ligands
Adenosine Diphosphate
1 Citation (Scopus)

Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry

Cummins, T. D. & Sapkota, G. P., 1 Jan 2018, Tissue Proteomics: Methods and Protocols. Sarwal, M. & Sigel, T. (eds.). New York: Humana Press, Vol. 1788. p. 43-61 19 p. (Methods in Molecular Biology; vol. 1788).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Mass Spectrometry
Green Fluorescent Proteins
Proteomics
Proteins
Tetracycline

Generation of endogenous BMP transcriptional reporter cells through CRISPR/Cas9 genome editing

Hutchinson, L. D., Bozatzi, P., Macartney, T. & Sapkota, G. P., 2018, Bone Morphogenetic Proteins: Methods and Protocols. Rogers, M. B. (ed.). New York: Humana Press, Vol. 1891. p. 29-35 7 p. (Methods in Molecular Biology; vol. 1891).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Clustered Regularly Interspaced Short Palindromic Repeats
Bone Morphogenetic Proteins
Chromatin
Complementary DNA
Firefly Luciferases

Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation

Hawley, S. A., Fyffe, F. A., Russell, F. M., Gowans, G. J. & Hardie, D. G., 2018, AMPK : Methods and Protocols. Neumann, D. & Viollet, B. (eds.). New York: Humana Press, p. 239-253 15 p. (Methods in Molecular Biology; vol. 1732).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

AMP-Activated Protein Kinases
Adenosine Monophosphate
Phosphotransferases
Tissue and Organ Harvesting
Adenosine Triphosphate
2017
3 Citations (Scopus)

Chromatin Replication and Histone Dynamics

Alabert, C., Jasencakova, Z. & Groth, A., 2017, DNA Replication : From Old Principles to New Discoveries. Masai, H. & Foiani, M. (eds.). Singapore: Springer Nature, p. 311-333 23 p. (Advances in Experimental Medicine and Biology; vol. 1042).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Histones
Chromatin
DNA Replication
Nucleosomes
Genomic Instability

Defining Lipoprotein Localisation by Fluorescence Microscopy

Casabona, M. G., Robert-Genthon, M., Grunwald, D. & Attrée, I., 2017, Bacterial Protein Secretion Systems: Methods and Protocols. Journet, L. & Cascales, E. (eds.). New York: Springer , p. 65-74 10 p. (Methods in Molecular Biology; vol. 1615).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Fluorescence Microscopy
Lipoproteins
Gram-Negative Bacteria
Spheroplasts
Proteins
15 Citations (Scopus)

Euglena gracilis Genome and Transcriptome: Organelles, Nuclear Genome Assembly Strategies and Initial Features

Ebenezer, T. E., Carrington, M., Lebert, M., Kelly, S. & Field, M. C., 21 Apr 2017, Euglena: Biochemistry, Cell and Molecular Biology. Schwartzbach, S. D. & Shigeoka, S. (eds.). Switzerland: Springer International Publishing, p. 125-140 16 p. (Advances in Experimental Medicine and Biology; vol. 979).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Euglena gracilis
Transcriptome
Organelles
Genome
Plastid Genomes
2 Citations (Scopus)

Isolation and western blotting of latex-bead phagosomes to track phagosome maturation

Härtlova, A., Peltier, J., Bilkei-Gorzo, O. & Trost, M., 2017, Phagocytosis and phagosomes: methods and protocols. Botelho, R. (ed.). New York: Springer , p. 241-248 8 p. (Methods in molecular biology; vol. 1519).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phagosomes
Microspheres
Western Blotting
Endosomes
Lysosomes
2 Citations (Scopus)

Probing the Role of Cyclic di-GMP Signaling Systems in Disease Using Chinese Radish

An, S-Q., Tang, J-L. & Dow, J. M., Sep 2017, c-di-GMP Signaling: Methods and Protocols. Sauer, K. (ed.). New York: Springer , Vol. 1657. p. 205-212 8 p. (Methods in Molecular Biology; vol. 1657).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Xanthomonas campestris
Raphanus
Virulence
Genome
Xylem
1 Citation (Scopus)

Profiling phosphopeptide-binding domain recognition specificity using peptide microarrays

Tinti, M., Panni, S. & Cesareni, G., 2017, Small molecule microarrays: methods and protocols. Uttamchandani, M. & Yao, S. Q. (eds.). New York: Springer , p. 177-193 17 p. (Methods in molecular biology; vol. 1518).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phosphopeptides
Phosphorylation
Peptides
Proteins
Threonine

Quantitative Determination of Anti-bacterial Activity During Bacterial Co-culture

Alcoforado Diniz, J., Hollmann, B. & Coulthurst, S. J., 2017, Bacterial Protein Secretion Systems: Methods and Protocols. Journet, L. & Cascales, E. (eds.). New York: Springer , p. 517-524 8 p. (Methods in Molecular Biology; vol. 1615).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Coculture Techniques
Bacteria
Bacterial Toxins
Serratia marcescens
Agar
8 Citations (Scopus)

Small-Molecule Inhibitors of LRRK2

Hatcher, J. M., Choi, H. G., Alessi, D. R. & Gray, N. S., 2017, Leucine-Rich Repeat Kinase 2 (LRRK2). Rideout, H. J. (ed.). Springer International Publishing, p. 241-264 24 p. (Advances in neurobiology; vol. 14).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leucine
Phosphotransferases
Parkinson Disease
Mutation
Protein Kinases
6 Citations (Scopus)
126 Downloads (Pure)

Uranium Bioreduction and Biomineralization

Wufuer, R., Wei, Y., Lin, Q., Wang, H., Song, W., Liu, W., Zhang, D., Pan, X. & Gadd, G. M., 2017, Advances in Applied Microbiology. Gadd, G. M. & Sariaslani, S. (eds.). Academic Press, Vol. 101. p. 137-168 32 p. (Advances in Applied Microbiology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Uranium
Environmental Biodegradation
Technology
Soil
Costs and Cost Analysis
2016
2 Citations (Scopus)

A fluorescent in vitro assay to investigate paralog-specific SUMO conjugation

Eisenhardt, N., Chaugule, V. K. & Pichler, A., 2016, SUMO: methods and protocols. Rodriguez, M. S. (ed.). New York: Springer , Vol. 1475. p. 67-78 12 p. (Methods in molecular biology; vol. 1475).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Sumoylation
Small Ubiquitin-Related Modifier Proteins
Ubiquitin-Protein Ligases
Ubiquitin
Enzymes

Analysis of mass spectrometry data for nucleolar proteomics experiments

Nicolas, A., Bensaddek, D. & Lamond, A. I., 2016, The nucleolus: methods and protocols. Németh, A. (ed.). New York: Springer , p. 263-276 14 p. (Methods in molecular biology; vol. 1455).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Proteomics
Mass Spectrometry
Workflow
Proteins
Research Personnel

Assays to measure PTEN lipid phosphatase activity in vitro from purified enzyme or immunoprecipitates

Spinelli, L. & Leslie, N. R., 2016, Protein tyrosine phosphatases: methods and protocols. Pulido, R. (ed.). New York: Springer , p. 95-105 11 p. (Methods in molecular biology; vol. 1447).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

PTEN Phosphohydrolase
Lipids
Enzymes
Phosphoric Monoester Hydrolases
Biological Phenomena
6 Citations (Scopus)

Assessing drought responses using thermal infrared imaging

Prashar, A. & Jones, H. G., 2016, Environmental responses in plants: methods and protocols. Duque, P. (ed.). New York: Springer , p. 209-219 11 p. (Methods in molecular biology; vol. 1398).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Droughts
Hot Temperature
Temperature
Controlled Environment
Plant Leaves
3 Citations (Scopus)

Assessing the phagosome proteome by quantitative mass spectrometry

Peltier, J., Härtlova, A. & Trost, M., 2016, Phagocytosis and phagosomes: methods and protocols. Botelho, R. (ed.). New York: Springer , p. 249-263 15 p. (Methods in molecular biology; vol. 1519).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phagosomes
Proteome
Mass Spectrometry
Innate Immunity
Proteomics
4 Citations (Scopus)

Cell cycle synchronization in xenopus egg extracts

Gillespie, P. J., Neusiedler, J., Creavin, K., Chadha, G. S. & Blow, J., 2016, Cell cycle oscillators: methods and protocols. Coutts, A. S. & Weston, L. (eds.). New York: Humana Press, p. 101-147 47 p. (Methods in Molecular Biology; vol. 1342).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Xenopus
Ovum
Cell Cycle
Eukaryotic Cells
Cell-Free System

Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) export pathway by live cell imaging methods

MacLean, L., Price, H. & O'Toole, P., 2016, Unconventional protein secretion: methods and protocols. Pompa, A. & De Marchis, F. (eds.). New York: Springer , Vol. 1459. p. 191-203 13 p. (Methods in Molecular Biology; vol. 1459).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leishmania
Membrane Proteins
Parasites
Leishmania major
Acylation

Fungi and Industrial Pollutants

Gadd, G., 19 Mar 2016, Environmental and Microbial Relationships. Druzhinina, I. S. & Kubicek, C. P. (eds.). 3 ed. Switzerland: Springer , Vol. 4. p. 99-125 27 p. (The Mycota; vol. 4).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

fungus
organic pollutant
pollutant
xenobiotics
bioremediation

Geomycology

Gadd, G., 2016, Fungal Applications in Sustainable Environmental Biotechnology. Purchase, D. (ed.). Switzerland: Springer , p. 371-401 31 p. (Fungal Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

fungus
metal
mineral
radionuclide
geomicrobiology
7 Citations (Scopus)

High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes

Obado, S. O., Field, M. C., Chait, B. T. & Rout, M. P., 2016, The Nuclear Envelope. Shackleton, S., Collas, P. & Schirmer, E. C. (eds.). New York: Humana Press, p. 67-80 14 p. (Methods in Molecular Biology; vol. 1411).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Trypanosomiasis
Nuclear Envelope
Nuclear Proteins
Protein Interaction Maps
Nuclear Pore
2 Citations (Scopus)

Imaging functional nucleic acid delivery to skin

Kaspar, R. L., Hickerson, R. P., González-González, E., Flores, M. A., Speaker, T. P., Rogers, F. A., Milstone, L. M. & Contag, C. H., 2016, RNA Imaging: methods and protocols. Medarova, Z. (ed.). New York: Springer , p. 1-24 24 p. (Methods in molecular biology; vol. 1372).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Nucleic Acids
Skin
Reporter Genes
Small Interfering RNA
Optical Imaging
3 Citations (Scopus)

Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells

Applewhite, D. A., Davis, C. A., Griffis, E. R. & Quintero, O. A., 2016, Cytoskeleton methods and protocols. Gavin, R. H. (ed.). 3rd ed. Humana Press, p. 83-97 15 p. (Methods in molecular biology; vol. 1365).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

RNA Interference
Cytoskeleton
Drosophila
Cell Culture Techniques
Fluorescence Microscopy
3 Citations (Scopus)

Insights into biological complexity from simple foundations

Albergante, L., Liu, D., Palmer, S. & Newman, T. J., 19 May 2016, Biophysics of infection. Leake, M. C. (ed.). Switzerland: Springer International Publishing, p. 295-305 11 p. (Advances in experimental medicine and biology; vol. 915).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Gene Regulatory Networks
Physics
DNA Replication
5 Citations (Scopus)

Monitoring alternative splicing changes in Arabidopsis circadian clock genes

Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S. & Staiger, D., 2016, Environmental responses in plants: methods and protocols. Duque, P. (ed.). New York: Springer , p. 119-132 14 p. (Methods in molecular biology; vol. 1398).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Circadian Clocks
Alternative Splicing
Arabidopsis
Ecotype
Gene Expression Regulation
22 Citations (Scopus)

Plant-microbiota interactions as a driver of the mineral turnover in the Rhizosphere

Alegria Terrazas, R., Giles, C., Paterson, E., Robertson-Albertyn, S., Cesco, S., Mimmo, T., Pii, Y. & Bulgarelli, D., 2016, Advances in applied microbiology. Sariaslani, S. & Gadd, G. M. (eds.). Academic Press, Vol. 95. p. 1-67 67 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Rhizosphere
Microbiota
Minerals
Soil
Agriculture
4 Citations (Scopus)

Quantitative proteomic analysis of the human nucleolus

Bensaddek, D., Nicolas, A. & Lamond, A. I., 2016, The nucleolus: methods and protocols. Németh, A. (ed.). New York: Springer , p. 249-262 14 p. (Methods in molecular biology; vol. 1455).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Proteome
Proteomics
Mass Spectrometry
Workflow
Cellular Structures
11 Citations (Scopus)

Tablet: visualizing next-generation sequence assemblies and mappings

Milne, I., Bayer, M., Stephen, G., Cardle, L. & Marshall, D., 2016, Plant bioinformatics: methods and protocols. Edwards, D. (ed.). New York: Springer , p. 253-268 16 p. (Methods in molecular biology; vol. 1374).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Tablets
Audiovisual Aids
Single Nucleotide Polymorphism
30 Citations (Scopus)

The Model [NiFe]-Hydrogenases of Escherichia coli

Sargent, F., 5 May 2016, Advances in Bacterial Electron Transport Systems and Their Regulation. Poole, R. K. (ed.). London: Elsevier, p. 433-507 75 p. (Advances in Microbial Physiology; vol. 68).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hydrogen
Escherichia coli
Isoenzymes
formic acid
Membranes
5 Citations (Scopus)

Tools to study SUMO conjugation in Caenorhabditis elegans

Pelisch, F. & Hay, R. T., 2016, SUMO: methods and protocols. Rodriguez, M. S. (ed.). New York: Springer , p. 233-256 24 p. (Methods in molecular biology; vol. 1475).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Sumoylation
Caenorhabditis elegans
Biochemistry
Ubiquitin-Protein Ligases
Cell Biology
2015
9 Citations (Scopus)

Detection of the bacterial potato pathogens Pectobacterium and Dickeya spp. Using conventional and real-time PCR

Humphris, S. N., Cahill, G., Elphinstone, J. G., Kelly, R., Parkinson, N. M., Pritchard, L., Toth, I. K. & Saddler, G. S., 2015, Plant pathology: techniques and protocols. Lacomme, C. (ed.). 2nd ed. New York: Humana Press, p. 1-16 16 p. (Methods in molecular biology; vol. 1302).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Pectobacterium
Solanum tuberosum
Real-Time Polymerase Chain Reaction
Seeds
Epidemiology
21 Citations (Scopus)

EPR distance measurements in deuterated proteins

El Mkami, H. & Norman, D. G., 2015, Electron paramagnetic resonance investigations of biological systems by using spin labels, spin probes, and intrinsic metal ions, Part B. Qin, P. Z. & Warncke, K. (eds.). Academic Press, p. 125-152 28 p. (Methods in enzymology; vol. 564).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Electrons
Proteins
Electron Spin Resonance Spectroscopy
Artifacts
12 Citations (Scopus)

Surface plasmon resonance analysis of seven-transmembrane receptors

Aristotelous, T., Hopkins, A. L. & Navratilova, I., 2015, Membrane proteins—production and functional characterization. Shukla, A. K. (ed.). Academic Press, p. 499-525 27 p. (Methods in Enzymology; vol. 556).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Surface Plasmon Resonance
Surface plasmon resonance
Ligands
G-Protein-Coupled Receptors
Screening
2014
6 Citations (Scopus)

In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Boevink, P., McLellan, H., Bukharova, T., Engelhardt, S. & Birch, P., 15 Feb 2014, Plant-pathogen interactions: methods and protocols. Birch, P., Jones, J. T. & Bos, J. I. B. (eds.). New York: Humana Press, p. 81-90 10 p. (Methods in Molecular Biology; vol. 1127).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Fluorescence
Proteins
Plant Cells
Confocal Microscopy

Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids

Rodriguez, P. A., Hogenhout, S. A. & Bos, J. I. B., 2014, Plant-pathogen interactions: methods and protocols. Birch, P., Jones, J. T. & Bos, J. I. B. (eds.). Humana Press, p. 137-143 7 p. (Methods in Molecular Biology; vol. 1127).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Aphids
Tobacco
RNA Interference
Computational Biology
Transcriptome
2 Citations (Scopus)

Structure-based virtual screening for the identification of RNA-binding ligands

Daldrop, P. & Brenk, R., 2014, Therapeutic applications of ribozymes and riboswitches: methods and protocols. Lafontaine, D. & Dubé, A. (eds.). Humana Press, p. 127-139 13 p. (Methods in Molecular Biology; vol. 1103).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

RNA
Ligands
Software
Proteins
2013
2 Citations (Scopus)

Assaying protein s-acylation in plants

Hemsley, P. A., 2013, G protein-couples receptor signaling in plants: methods and protocols. Running, M. P. (ed.). Humana Press, p. 141-146 6 p. (Methods in Molecular Biology; vol. 1043).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Acylation
Proteins
Heterotrimeric GTP-Binding Proteins
Protein S
Post Translational Protein Processing
35 Citations (Scopus)

Biophysical screening for the discovery of small-molecule ligands

Ciulli, A., 2013, Protein-ligand interactions: methods and applications. Williams, M. A. & Daviter, T. (eds.). Humana Press, p. 357-388 32 p. (Methods in Molecular Biology; vol. 1008).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Ligands
Fluorometry
Calorimetry
Proteins
Fluorescence Polarization
5 Citations (Scopus)

Creation and characterization of glycosyltransferase mutants of trypanosoma brucei

Izquierdo, L., Güther, M. L. S. & Ferguson, M. A. J., 2013, Glycosyltransferases. Brockhausen, I. (ed.). Humana Press, p. 249-275 27 p. (Methods in Molecular Biology; vol. 1022).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Glycosyltransferases
Trypanosoma brucei brucei
Glycoconjugates
Life Cycle Stages
Parasites