School of Life Sciences

  • United Kingdom

Research Output 1968 2020

Filter
Chapter (peer-reviewed)
2019

A Modular Tray Growth System for Barley

Arrieta, M., Colas, I., Macaulay, M., Waugh, R. & Ramsay, L., 4 Oct 2019, Plant Meiosis: Methods and Protocols. Pradillo, M. & Heckmann, S. (eds.). New York: Humana Press, p. 367-379 13 p. (Methods in Molecular Biology; vol. 2061).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hordeum
Meiosis
Growth
Genetic Recombination
Cell Biology

A Practical Guide to Barley Crossing

Thomas, W. T. B., Bull, H., Booth, A., Hamilton, R., Forster, B. P. & Franckowiak, J. D., 1 Jan 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 21-36 16 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hordeum
Inbreeding
Pollen
Seeds

Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods

Hurst, C. H., Turnbull, D. & Hemsley, P. A., 2019, Protein Lipidation: Methods and Protocols. Linder, M. E. (ed.). New York: Springer , Vol. 2009. p. 3-11 9 p. (Methods in Molecular Biology; vol. 2009).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Acylation
Protein S
Proteins
Post Translational Protein Processing
Cysteine

DiCre-Based Inducible Disruption of Leishmania Genes

Duncan, S. M., Myburgh, E., Alves-Ferreira, E. V. & Mottram, J. C., 2019, Leishmania: Methods and Protocols. Clos, J. (ed.). New York: Humana Press, Vol. 1971. p. 211-224 14 p. (Methods in Molecular Biology; vol. 1971).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leishmania
Essential Genes
Gene Deletion
Genes
Enzyme Activation
8 Downloads (Pure)

Exome Capture for Variant Discovery and Analysis in Barley

Bayer, M., Morris, J. A., Booth, C., Booth, A., Uzrek, N., Russell, J. R., Waugh, R. & Hedley, P. E., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 283-310 28 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Exome
Hordeum
Genome
Firearms
Computational Biology

Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy

Darrier, B., Arrieta, M., Mittmann, S. U., Sourdille, P., Ramsay, L., Waugh, R. & Colas, I., 4 Oct 2019, Plant Meiosis: Methods and Protocols. Pradillo, M. & Heckmann, S. (eds.). New York: Humana Press, p. 207-215 9 p. (Methods in Molecular Biology; vol. 2061).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Synaptonemal Complex
Hordeum
Genetic Recombination
Triticum
Microscopy
9 Downloads (Pure)

High-Resolution RT-PCR Analysis of Alternative Barley Transcripts

Simpson, C. G., Fuller, J., Rapazote-Flores, P., Mayer, C. D., Calixto, C. P. G., Milne, L., Hedley, P. E., Booth, C., Waugh, R. & Brown, J. W. S., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 269-281 13 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Hordeum
RNA
Polymerase Chain Reaction
Protein Isoforms
Genome
3 Citations (Scopus)

Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

McWilliams, T. G. & Ganley, I. G., 2019, Autophagy: Methods and Protocols. Ktistakis, N. & Florey, O. (eds.). New York: Humana Press, Vol. 1880. p. 621-642 22 p. (Methods in Molecular Biology; vol. 1880).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Mitochondrial Degradation
Quality Control
Autophagy
Organelles
Homeostasis

Methods to analyze STUbL activity

Branigan, E., Plechanovová, A. & Hay, R. T., 2019, Methods in Enzymology. Hochstrasser, M. (ed.). Cambridge, MA: Elsevier, Vol. 618. p. 257-280 24 p. (Methods in Enzymology; vol. 618).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Ubiquitin-Protein Ligases
Ubiquitin
Genes
Assays
Substrates
1 Citation (Scopus)
7 Downloads (Pure)

Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

Mittmann, S., Arrieta, M., Ramsay, L., Waugh, R. & Colas, I., 2019, Barley: Methods and Protocols. Harwood, W. A. (ed.). New York: Humana Press, Vol. 1900. p. 167-179 13 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Hordeum
Pollen
Microscopy
Stem Cells
Genetic Recombination
2018
1 Citation (Scopus)

Biochemical and Structural Properties of Fungal Holliday Junction-Resolving Enzymes

Liu, Y., Freeman, A., Déclais, A-C., Gartner, A. & Lilley, D. M. J., 2018, Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination. Spies, M. & Malkova, A. (eds.). Academic Press, Vol. 600. p. 543-568 26 p. (Methods in Enzymology; vol. 600).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Cruciform DNA
Structural properties
Enzymes
Chaetomium
DNA
1 Citation (Scopus)

Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK

Fyffe, F. A., Hawley, S. A., Gray, A. & Hardie, D. G., 2018, AMPK : Methods and Protocols. Neumann, D. & Viollet, B. (eds.). New York: Humana Press, p. 69-86 18 p. (Methods in Molecular Biology; vol. 1732).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

AMP-Activated Protein Kinases
Adenosine Triphosphate
Adenosine Monophosphate
Ligands
Adenosine Diphosphate
1 Citation (Scopus)

Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry

Cummins, T. D. & Sapkota, G. P., 1 Jan 2018, Tissue Proteomics: Methods and Protocols. Sarwal, M. & Sigel, T. (eds.). New York: Humana Press, Vol. 1788. p. 43-61 19 p. (Methods in Molecular Biology; vol. 1788).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Mass Spectrometry
Green Fluorescent Proteins
Proteomics
Proteins
Tetracycline
13 Downloads (Pure)

Generation of endogenous BMP transcriptional reporter cells through CRISPR/Cas9 genome editing

Hutchinson, L. D., Bozatzi, P., Macartney, T. & Sapkota, G. P., 2018, Bone Morphogenetic Proteins: Methods and Protocols. Rogers, M. B. (ed.). New York: Humana Press, Vol. 1891. p. 29-35 7 p. (Methods in Molecular Biology; vol. 1891).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Clustered Regularly Interspaced Short Palindromic Repeats
Bone Morphogenetic Proteins
Chromatin
Complementary DNA
Firefly Luciferases

Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation

Hawley, S. A., Fyffe, F. A., Russell, F. M., Gowans, G. J. & Hardie, D. G., 2018, AMPK : Methods and Protocols. Neumann, D. & Viollet, B. (eds.). New York: Humana Press, p. 239-253 15 p. (Methods in Molecular Biology; vol. 1732).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

AMP-Activated Protein Kinases
Adenosine Monophosphate
Phosphotransferases
Tissue and Organ Harvesting
Adenosine Triphosphate
2017
6 Citations (Scopus)

Chromatin Replication and Histone Dynamics

Alabert, C., Jasencakova, Z. & Groth, A., 2017, DNA Replication : From Old Principles to New Discoveries. Masai, H. & Foiani, M. (eds.). Singapore: Springer Nature, p. 311-333 23 p. (Advances in Experimental Medicine and Biology; vol. 1042).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Histones
Chromatin
DNA Replication
Nucleosomes
Genomic Instability

Defining Lipoprotein Localisation by Fluorescence Microscopy

Casabona, M. G., Robert-Genthon, M., Grunwald, D. & Attrée, I., 2017, Bacterial Protein Secretion Systems: Methods and Protocols. Journet, L. & Cascales, E. (eds.). New York: Springer , p. 65-74 10 p. (Methods in Molecular Biology; vol. 1615).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Fluorescence Microscopy
Lipoproteins
Gram-Negative Bacteria
Spheroplasts
Proteins
16 Citations (Scopus)

Euglena gracilis Genome and Transcriptome: Organelles, Nuclear Genome Assembly Strategies and Initial Features

Ebenezer, T. E., Carrington, M., Lebert, M., Kelly, S. & Field, M. C., 21 Apr 2017, Euglena: Biochemistry, Cell and Molecular Biology. Schwartzbach, S. D. & Shigeoka, S. (eds.). Switzerland: Springer International Publishing, p. 125-140 16 p. (Advances in Experimental Medicine and Biology; vol. 979).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Euglena gracilis
Transcriptome
Organelles
Genome
Plastid Genomes
2 Citations (Scopus)

Isolation and western blotting of latex-bead phagosomes to track phagosome maturation

Härtlova, A., Peltier, J., Bilkei-Gorzo, O. & Trost, M., 2017, Phagocytosis and phagosomes: methods and protocols. Botelho, R. (ed.). New York: Springer , p. 241-248 8 p. (Methods in molecular biology; vol. 1519).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phagosomes
Microspheres
Western Blotting
Endosomes
Lysosomes
2 Citations (Scopus)

Probing the Role of Cyclic di-GMP Signaling Systems in Disease Using Chinese Radish

An, S-Q., Tang, J-L. & Dow, J. M., Sep 2017, c-di-GMP Signaling: Methods and Protocols. Sauer, K. (ed.). New York: Springer , Vol. 1657. p. 205-212 8 p. (Methods in Molecular Biology; vol. 1657).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Xanthomonas campestris
Raphanus
Virulence
Genome
Xylem
1 Citation (Scopus)

Profiling phosphopeptide-binding domain recognition specificity using peptide microarrays

Tinti, M., Panni, S. & Cesareni, G., 2017, Small molecule microarrays: methods and protocols. Uttamchandani, M. & Yao, S. Q. (eds.). New York: Springer , p. 177-193 17 p. (Methods in molecular biology; vol. 1518).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phosphopeptides
Phosphorylation
Peptides
Proteins
Threonine

Quantitative Determination of Anti-bacterial Activity During Bacterial Co-culture

Alcoforado Diniz, J., Hollmann, B. & Coulthurst, S. J., 2017, Bacterial Protein Secretion Systems: Methods and Protocols. Journet, L. & Cascales, E. (eds.). New York: Springer , p. 517-524 8 p. (Methods in Molecular Biology; vol. 1615).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Coculture Techniques
Bacteria
Bacterial Toxins
Serratia marcescens
Agar
10 Citations (Scopus)

Small-Molecule Inhibitors of LRRK2

Hatcher, J. M., Choi, H. G., Alessi, D. R. & Gray, N. S., 2017, Leucine-Rich Repeat Kinase 2 (LRRK2). Rideout, H. J. (ed.). Springer International Publishing, p. 241-264 24 p. (Advances in neurobiology; vol. 14).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leucine
Phosphotransferases
Parkinson Disease
Mutation
Protein Kinases
7 Citations (Scopus)
169 Downloads (Pure)

Uranium Bioreduction and Biomineralization

Wufuer, R., Wei, Y., Lin, Q., Wang, H., Song, W., Liu, W., Zhang, D., Pan, X. & Gadd, G. M., 2017, Advances in Applied Microbiology. Gadd, G. M. & Sariaslani, S. (eds.). Academic Press, Vol. 101. p. 137-168 32 p. (Advances in Applied Microbiology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Open Access
File
Uranium
Environmental Biodegradation
Technology
Soil
Costs and Cost Analysis
2016
2 Citations (Scopus)

A fluorescent in vitro assay to investigate paralog-specific SUMO conjugation

Eisenhardt, N., Chaugule, V. K. & Pichler, A., 2016, SUMO: methods and protocols. Rodriguez, M. S. (ed.). New York: Springer , Vol. 1475. p. 67-78 12 p. (Methods in molecular biology; vol. 1475).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Sumoylation
Small Ubiquitin-Related Modifier Proteins
Ubiquitin-Protein Ligases
Ubiquitin
Enzymes

Analysis of mass spectrometry data for nucleolar proteomics experiments

Nicolas, A., Bensaddek, D. & Lamond, A. I., 2016, The nucleolus: methods and protocols. Németh, A. (ed.). New York: Springer , p. 263-276 14 p. (Methods in molecular biology; vol. 1455).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Proteomics
Mass Spectrometry
Workflow
Proteins
Research Personnel

Assays to measure PTEN lipid phosphatase activity in vitro from purified enzyme or immunoprecipitates

Spinelli, L. & Leslie, N. R., 2016, Protein tyrosine phosphatases: methods and protocols. Pulido, R. (ed.). New York: Springer , p. 95-105 11 p. (Methods in molecular biology; vol. 1447).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

PTEN Phosphohydrolase
Lipids
Enzymes
Phosphoric Monoester Hydrolases
Biological Phenomena
7 Citations (Scopus)

Assessing drought responses using thermal infrared imaging

Prashar, A. & Jones, H. G., 2016, Environmental responses in plants: methods and protocols. Duque, P. (ed.). New York: Springer , p. 209-219 11 p. (Methods in molecular biology; vol. 1398).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Droughts
Hot Temperature
Temperature
Controlled Environment
Plant Leaves
3 Citations (Scopus)

Assessing the phagosome proteome by quantitative mass spectrometry

Peltier, J., Härtlova, A. & Trost, M., 2016, Phagocytosis and phagosomes: methods and protocols. Botelho, R. (ed.). New York: Springer , p. 249-263 15 p. (Methods in molecular biology; vol. 1519).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Phagosomes
Proteome
Mass Spectrometry
Innate Immunity
Proteomics
5 Citations (Scopus)

Cell cycle synchronization in xenopus egg extracts

Gillespie, P. J., Neusiedler, J., Creavin, K., Chadha, G. S. & Blow, J., 2016, Cell cycle oscillators: methods and protocols. Coutts, A. S. & Weston, L. (eds.). New York: Humana Press, p. 101-147 47 p. (Methods in Molecular Biology; vol. 1342).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Xenopus
Ovum
Cell Cycle
Eukaryotic Cells
Cell-Free System

Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) export pathway by live cell imaging methods

MacLean, L., Price, H. & O'Toole, P., 2016, Unconventional protein secretion: methods and protocols. Pompa, A. & De Marchis, F. (eds.). New York: Springer , Vol. 1459. p. 191-203 13 p. (Methods in Molecular Biology; vol. 1459).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Leishmania
Membrane Proteins
Parasites
Leishmania major
Acylation

Fungi and Industrial Pollutants

Gadd, G., 19 Mar 2016, Environmental and Microbial Relationships. Druzhinina, I. S. & Kubicek, C. P. (eds.). 3 ed. Switzerland: Springer , Vol. 4. p. 99-125 27 p. (The Mycota; vol. 4).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

fungus
organic pollutant
pollutant
xenobiotics
bioremediation

Geomycology

Gadd, G., 2016, Fungal Applications in Sustainable Environmental Biotechnology. Purchase, D. (ed.). Switzerland: Springer , p. 371-401 31 p. (Fungal Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

fungus
metal
mineral
radionuclide
geomicrobiology
8 Citations (Scopus)

High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes

Obado, S. O., Field, M. C., Chait, B. T. & Rout, M. P., 2016, The Nuclear Envelope. Shackleton, S., Collas, P. & Schirmer, E. C. (eds.). New York: Humana Press, p. 67-80 14 p. (Methods in Molecular Biology; vol. 1411).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Trypanosomiasis
Nuclear Envelope
Nuclear Proteins
Protein Interaction Maps
Nuclear Pore
2 Citations (Scopus)

Imaging functional nucleic acid delivery to skin

Kaspar, R. L., Hickerson, R. P., González-González, E., Flores, M. A., Speaker, T. P., Rogers, F. A., Milstone, L. M. & Contag, C. H., 2016, RNA Imaging: methods and protocols. Medarova, Z. (ed.). New York: Springer , p. 1-24 24 p. (Methods in molecular biology; vol. 1372).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Nucleic Acids
Skin
Reporter Genes
Small Interfering RNA
Optical Imaging
3 Citations (Scopus)

Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells

Applewhite, D. A., Davis, C. A., Griffis, E. R. & Quintero, O. A., 2016, Cytoskeleton methods and protocols. Gavin, R. H. (ed.). 3rd ed. Humana Press, p. 83-97 15 p. (Methods in molecular biology; vol. 1365).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

RNA Interference
Cytoskeleton
Drosophila
Cell Culture Techniques
Fluorescence Microscopy
3 Citations (Scopus)

Insights into biological complexity from simple foundations

Albergante, L., Liu, D., Palmer, S. & Newman, T. J., 19 May 2016, Biophysics of infection. Leake, M. C. (ed.). Switzerland: Springer International Publishing, p. 295-305 11 p. (Advances in experimental medicine and biology; vol. 915).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Gene Regulatory Networks
Physics
DNA Replication
6 Citations (Scopus)

Monitoring alternative splicing changes in Arabidopsis circadian clock genes

Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S. & Staiger, D., 2016, Environmental responses in plants: methods and protocols. Duque, P. (ed.). New York: Springer , p. 119-132 14 p. (Methods in molecular biology; vol. 1398).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Circadian Clocks
Alternative Splicing
Arabidopsis
Ecotype
Gene Expression Regulation
24 Citations (Scopus)

Plant-microbiota interactions as a driver of the mineral turnover in the Rhizosphere

Alegria Terrazas, R., Giles, C., Paterson, E., Robertson-Albertyn, S., Cesco, S., Mimmo, T., Pii, Y. & Bulgarelli, D., 2016, Advances in applied microbiology. Sariaslani, S. & Gadd, G. M. (eds.). Academic Press, Vol. 95. p. 1-67 67 p.

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Rhizosphere
Microbiota
Minerals
Soil
Agriculture
4 Citations (Scopus)

Quantitative proteomic analysis of the human nucleolus

Bensaddek, D., Nicolas, A. & Lamond, A. I., 2016, The nucleolus: methods and protocols. Németh, A. (ed.). New York: Springer , p. 249-262 14 p. (Methods in molecular biology; vol. 1455).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Proteome
Proteomics
Mass Spectrometry
Workflow
Cellular Structures
11 Citations (Scopus)

Tablet: visualizing next-generation sequence assemblies and mappings

Milne, I., Bayer, M., Stephen, G., Cardle, L. & Marshall, D., 2016, Plant bioinformatics: methods and protocols. Edwards, D. (ed.). New York: Springer , p. 253-268 16 p. (Methods in molecular biology; vol. 1374).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Tablets
Audiovisual Aids
Single Nucleotide Polymorphism
33 Citations (Scopus)

The Model [NiFe]-Hydrogenases of Escherichia coli

Sargent, F., 5 May 2016, Advances in Bacterial Electron Transport Systems and Their Regulation. Poole, R. K. (ed.). London: Elsevier, p. 433-507 75 p. (Advances in Microbial Physiology; vol. 68).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Hydrogen
Escherichia coli
Isoenzymes
formic acid
Membranes
5 Citations (Scopus)

Tools to study SUMO conjugation in Caenorhabditis elegans

Pelisch, F. & Hay, R. T., 2016, SUMO: methods and protocols. Rodriguez, M. S. (ed.). New York: Springer , p. 233-256 24 p. (Methods in molecular biology; vol. 1475).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Sumoylation
Caenorhabditis elegans
Biochemistry
Ubiquitin-Protein Ligases
Cell Biology
2015
9 Citations (Scopus)

Detection of the bacterial potato pathogens Pectobacterium and Dickeya spp. Using conventional and real-time PCR

Humphris, S. N., Cahill, G., Elphinstone, J. G., Kelly, R., Parkinson, N. M., Pritchard, L., Toth, I. K. & Saddler, G. S., 2015, Plant pathology: techniques and protocols. Lacomme, C. (ed.). 2nd ed. New York: Humana Press, p. 1-16 16 p. (Methods in molecular biology; vol. 1302).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Pectobacterium
Solanum tuberosum
Real-Time Polymerase Chain Reaction
Seeds
Epidemiology
22 Citations (Scopus)

EPR distance measurements in deuterated proteins

El Mkami, H. & Norman, D. G., 2015, Electron paramagnetic resonance investigations of biological systems by using spin labels, spin probes, and intrinsic metal ions, Part B. Qin, P. Z. & Warncke, K. (eds.). Academic Press, p. 125-152 28 p. (Methods in enzymology; vol. 564).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Electrons
Proteins
Electron Spin Resonance Spectroscopy
Artifacts
12 Citations (Scopus)

Surface plasmon resonance analysis of seven-transmembrane receptors

Aristotelous, T., Hopkins, A. L. & Navratilova, I., 2015, Membrane proteins—production and functional characterization. Shukla, A. K. (ed.). Academic Press, p. 499-525 27 p. (Methods in Enzymology; vol. 556).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Surface Plasmon Resonance
Surface plasmon resonance
Ligands
G-Protein-Coupled Receptors
Screening
2014
6 Citations (Scopus)

In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats

Boevink, P., McLellan, H., Bukharova, T., Engelhardt, S. & Birch, P., 15 Feb 2014, Plant-pathogen interactions: methods and protocols. Birch, P., Jones, J. T. & Bos, J. I. B. (eds.). New York: Humana Press, p. 81-90 10 p. (Methods in Molecular Biology; vol. 1127).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Fluorescence
Proteins
Plant Cells
Confocal Microscopy

Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids

Rodriguez, P. A., Hogenhout, S. A. & Bos, J. I. B., 2014, Plant-pathogen interactions: methods and protocols. Birch, P., Jones, J. T. & Bos, J. I. B. (eds.). Humana Press, p. 137-143 7 p. (Methods in Molecular Biology; vol. 1127).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Aphids
Tobacco
RNA Interference
Computational Biology
Transcriptome
2 Citations (Scopus)

Structure-based virtual screening for the identification of RNA-binding ligands

Daldrop, P. & Brenk, R., 2014, Therapeutic applications of ribozymes and riboswitches: methods and protocols. Lafontaine, D. & Dubé, A. (eds.). Humana Press, p. 127-139 13 p. (Methods in Molecular Biology; vol. 1103).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

RNA
Ligands
Software
Proteins
2013
3 Citations (Scopus)

Assaying protein s-acylation in plants

Hemsley, P. A., 2013, G protein-couples receptor signaling in plants: methods and protocols. Running, M. P. (ed.). Humana Press, p. 141-146 6 p. (Methods in Molecular Biology; vol. 1043).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Acylation
Proteins
Heterotrimeric GTP-Binding Proteins
Protein S
Post Translational Protein Processing