Projects per year
Personal profile
Research
Nuclear structure and gene expression
We are studying structure/function relationships in mammalian cell nuclei, focussed on mechanisms of RNA processing, including alternative splicing of mRNA precursors and pre-rRNA processing in the nucleolus. Our major aim is to characterise the composition and function of subnuclear organelles and multiprotein complexes and to identify mechanisms regulating their assembly and turnover, particularly in human cells. The importance of understanding nuclear organisation is underlined by evidence showing that multiple human diseases, including inherited genetic disorders, malignancies and viral infections, modify or disrupt subnuclear bodies. Our studies employ a combination of quantitative techniques, including mass-spectrometry-based proteomics, fluorescence and electron microscopy and deep sequencing.
We are also taking a chemical biology approach to study RNA processing and nuclear organisation, involving our characterisation of novel small molecule modulators that affect RNA processing mechanisms and/or modify the structure and composition of subnuclear bodies. We have identified these chemical tools by screening libraries of drug-like small molecules, in collaboration with David Gray and colleagues in the SLS Drug Discovery Unit and have already identified protein targets for several of these small molecules. Work is underway to identify additional protein targets for our collection of novel small molecule modulators.
Another theme of our research is using quantitative, mass spectrometry-based proteomics to study stem cell biology. We have characterised protein expression across hundreds of independent iPSC lines derived from both healthy donors and from disease cohorts, which were generated in the HipSci consortium. Together with our collaborators we have linked protein expression levels with both genomic and transcriptome data from the same cell lines and mapped protein and peptide QTLs. We have identified that loss of X chromosome inactivation in iPSC lines derived from healthy female donors correlates strongly with loss of Xist RNA expression and discovered that this affects protein expression from many autosomal loci, as well as resulting in increased expression of both RNA and protein from genes on the reactivated X chromosome. We are continuing our studies on iPSC lines to determine how proteome expression varies between individuals across the human population and how this impacts on cell phenotypes, responses and disease mechanisms.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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Collaborations and top research areas from the last five years
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Characterising Splice Site Modulation Approaches For Huntington's Disease Therapeutics
1/06/23 → 31/05/25
Project: Research
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Harnessing the splicing code for targeted control of gene expression (UNLEASH)
1/06/23 → 31/05/29
Project: Research
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Protein Correlation Profiling Analysis Of The Nipah Virus-Host Interface (Fellowship)
1/02/22 → 31/01/25
Project: Research
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The Interplay Between Oxygen Sensors PHDs and the Cell Cycle (Joint with University of Liverpool)
Fleming, S., Lamond, A. & Swedlow, J.
1/09/17 → 31/08/24
Project: Research
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Native Size-Exclusion Chromatography-Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes
Samant, R. S., Batista, S., Larance, M., Ozer, B., Milton, C. I., Bludau, I., Wu, E., Biggins, L., Andrews, S., Hervieu, A., Johnston, H. E., Al-Lazikhani, B., Lamond, A. I., Clarke, P. A. & Workman, P., Feb 2023, In: Molecular & Cellular Proteomics. 22, 2, 22 p., 100485.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)20 Downloads (Pure) -
Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication
Alvarez, V., Bandau, S., Jiang, H., Rios-Szwed, D., Hukelmann, J., Garcia-Wilson, E., Wiechens, N., Griesser, E., Ten Have, S., Owen-Hughes, T., Lamond, A. & Alabert, C., 31 Jan 2023, In: Cell Reports. 42, 1, 22 p., 111996.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)37 Downloads (Pure) -
The immunological proteome resource
Brenes, A. J., Lamond, A. I. & Cantrell, D. A., May 2023, In: Nature Immunology. 24, 5, 1 p., 731.Research output: Contribution to journal › Letter › peer-review
2 Citations (Scopus) -
The Mission to Ensure Continued Funding for Excellent Basic Research
Lamond, A. I., Dikic, I., Nussenzweig, A., Muller, C. W., Thornton, J. M. & Yaffe, M. B., 5 Jul 2023, In: EMBO Reports. 24, 7, 2 p., e57498.Research output: Contribution to journal › Comment/debate › peer-review
Open AccessFile11 Downloads (Pure) -
The Nucleolus
Lamond, A. I., Ly, T., Hutten, S. & Nicolas, A., 2023, Encyclopedia of Cell Biology. Bradshaw, R. A., Hart, G. W., Stahl, P. D. & Dustin, M. L. (eds.). 2nd ed. London: Elsevier, Vol. 5. p. 26-43 18 p.Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia/dictionary
Activities
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CHDI Foundation: Workshop on "RNA-directed Strategies to Target HTT" in Los Angeles
Angus Lamond (Participant)
7 Dec 2022Activity: Participating in or organising an event types › Participation in workshop, seminar, course
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Lister Institute of Preventive Medicine (External organisation)
Angus Lamond (Member)
1 Nov 2022 → 30 Sept 2028Activity: Membership types › Membership of committee
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HTT RNA Structure, RNA Protein Interactors, and HTT Translation
Angus Lamond (Participant)
7 Dec 2022 → 8 Dec 2022Activity: Participating in or organising an event types › Participation in workshop, seminar, course
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Proteomics in Cell Biology + Disease Mechanisms
Angus Lamond (Organiser) & Angus Lamond (Speaker)
10 Oct 2022 → 12 Oct 2022Activity: Participating in or organising an event types › Participation in conference
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The Novo Nordisk Foundation Center for Protein Research (External organisation)
Angus Lamond (Member)
1 Jun 2022Activity: Membership types › Membership of panel