Projects per year
Personal profile
Research
Nuclear structure and gene expression
We are studying structure/function relationships in mammalian cell nuclei, focussed on mechanisms of RNA processing, including alternative splicing of mRNA precursors and pre-rRNA processing in the nucleolus. Our major aim is to characterise the composition and function of subnuclear organelles and multiprotein complexes and to identify mechanisms regulating their assembly and turnover, particularly in human cells. The importance of understanding nuclear organisation is underlined by evidence showing that multiple human diseases, including inherited genetic disorders, malignancies and viral infections, modify or disrupt subnuclear bodies. Our studies employ a combination of quantitative techniques, including mass-spectrometry-based proteomics, fluorescence and electron microscopy and deep sequencing.
We are also taking a chemical biology approach to study RNA processing and nuclear organisation, involving our characterisation of novel small molecule modulators that affect RNA processing mechanisms and/or modify the structure and composition of subnuclear bodies. We have identified these chemical tools by screening libraries of drug-like small molecules, in collaboration with David Gray and colleagues in the SLS Drug Discovery Unit and have already identified protein targets for several of these small molecules. Work is underway to identify additional protein targets for our collection of novel small molecule modulators.
Another theme of our research is using quantitative, mass spectrometry-based proteomics to study stem cell biology. We have characterised protein expression across hundreds of independent iPSC lines derived from both healthy donors and from disease cohorts, which were generated in the HipSci consortium. Together with our collaborators we have linked protein expression levels with both genomic and transcriptome data from the same cell lines and mapped protein and peptide QTLs. We have identified that loss of X chromosome inactivation in iPSC lines derived from healthy female donors correlates strongly with loss of Xist RNA expression and discovered that this affects protein expression from many autosomal loci, as well as resulting in increased expression of both RNA and protein from genes on the reactivated X chromosome. We are continuing our studies on iPSC lines to determine how proteome expression varies between individuals across the human population and how this impacts on cell phenotypes, responses and disease mechanisms.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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Protein Correlation Profiling Analysis Of The Nipah Virus-Host Interface (Fellowship)
1/02/22 → 31/01/25
Project: Research
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The Interplay Between Oxygen Sensors PHDs and the Cell Cycle (Joint with University of Liverpool)
Fleming, S., Lamond, A. & Swedlow, J.
1/09/17 → 31/08/24
Project: Research
Research output
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Author Correction: Cell fate decisions are specified by the dynamic ERK interactome
von Kriegsheim, A., Baiocchi, D., Birtwistle, M., Sumpton, D., Bienvenut, W., Morrice, N., Yamada, K., Lamond, A., Kalna, G., Orton, R., Gilbert, D. & Kolch, W., 18 Feb 2022, (E-pub ahead of print) In: Nature Cell Biology. 24, p. 400 1 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Identification of putative reader proteins of 5-methylcytosine and its derivatives in Caenorhabditis elegans RNA
Navarro, I. C., Man Suen, K., Bensaddek, D., Tanpure, A., Lamond, A., Balasubramanian, S. & Miska, E. A., 25 Jan 2022, (Submitted) BioRxiv, p. 1-23, 23 p.Research output: Working paper/Preprint › Preprint
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Nrf2 activation reprograms macrophage intermediary metabolism and suppresses the type I interferon response
Ryan, D. G., Knatko, E. V., Casey, A. M., Hukelmann, J., Dayalan Naidu, S., Brenes, A. J., Ekkunagul, T., Baker, C., Higgins, M., Tronci, L., Nikitopolou, E., Honda, T., Hartley, R. C., O'Neill, L. A. J., Frezza, C., Lamond, A. I., Abramov, A. Y., Arthur, J. S. C., Cantrell, D. A., Murphy, M. P. & 1 others, , 18 Feb 2022, In: iScience. 25, 2, 21 p., 103827.Research output: Contribution to journal › Article › peer-review
Open AccessFile18 Downloads (Pure) -
Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
Yoshikawa, H., Sundaramoorthy, R., Mariyappa, D., Jiang, H. & Lamond, A. I., 5 Aug 2021, In: Bio-Protocol. 11, 15, p. 1-15 15 p., e4106.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)45 Downloads (Pure) -
Erosion of human X chromosome inactivation causes major remodeling of the iPSC proteome
Brenes, A. J., Yoshikawa, H., Bensaddek, D., Mirauta, B., Seaton, D. D., Hukelmann, J., Jiang, H., Stegle, O. & Lamond, A., 27 Apr 2021, In: Cell Reports. 35, 4, 20 p., 109032.Research output: Contribution to journal › Article › peer-review
Open AccessFile5 Citations (Scopus)38 Downloads (Pure)
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Proteomic analyses of human stem cells
Angus Lamond (Speaker)
22 Oct 2021Activity: Talk or presentation types › Invited talk
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Cell Biology of the nucleus (3 lectures)
Angus Lamond (Speaker)
29 Nov 2021Activity: Talk or presentation types › Oral presentation
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Institute for Molecular Biology (IMB) in Mainz, Germany (External organisation)
Angus Lamond (Chair)
2021 → 2022Activity: Membership types › Membership of committee
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University of Oslo
Angus Lamond (Visiting researcher)
29 Nov 2021Activity: Visiting an external institution types › Visiting an external institution - other
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Proteomics analyses of human stem cells
Angus Lamond (Speaker)
14 Nov 2021 → 18 Nov 2021Activity: Talk or presentation types › Invited talk