Projects per year
Personal profile
Research
Mitotic chromosome dynamics
During cell division, the two copies of a cell's genetic material are completely separated and delivered to a pair of new daughter cells. Proper chromosome segregation requires the formation of correct attachments between the genetic material, assembled into chromosomes, and ends of microtubules. Our research is focussed on establishing the molecular mechanisms that mediate and monitor the correct attachment of chromosomes to microtubule at a special structure known as the kinetochore. To achieve this, we make use of advanced tools for imaging the components of cell division, especially in living cells and specialised mass spectrometry methods to probe the molecular machinery of cell division. Recently, we used these tools to discover a new protein, Bod1, that plays a critical role in the formation of correct attachments between microtubules and chromosomes by modulating the activity of Aurora B protein kinase. We recently installed a newly developed OMX microscope and are using this system to probe the inner workings of the centromere and kinetochore of the mitotic chromosome.
Like many cell biology labs, we generate large sets of image data. To manage, analyse and understand this data, we, long with our collaborators, formed the Open Microscopy Environment Consortium. OME develops data specifications, file format translators and data management software for imaging applications. OME tools are used in thousands of labs around the world. For more info on OME, see the Consortium's web site.
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- 13 Similar Profiles
Kinetochores
Medicine & Life Sciences
Cell Cycle
Medicine & Life Sciences
Proteins
Medicine & Life Sciences
Imaging techniques
Chemical Compounds
Microtubules
Medicine & Life Sciences
Chromosomes
Chemical Compounds
Microscopy
Medicine & Life Sciences
Cells
Chemical Compounds
Network
Recent external collaboration on country level. Dive into details by clicking on the dots.
Projects 2010 2023
The Image Data Resource: Making Biological Imaging Data FAIR (Joint with EMBL-EBI) (Biomedical Resources Grant)
1/10/18 → 30/09/21
Project: Research
The Interplay Between Oxygen Sensors PHDs and the Cell Cycle (Joint with University of Liverpool)
Fleming, S., Lamond, A. & Swedlow, J.
1/09/17 → 31/08/22
Project: Research
Research Output 2001 2019
Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope
McGloin, D., Swedlow, J. & Zdankowski, P., 1 Apr 2019, In : Biomedical Optics Express. 10, 4, p. 1999-2009 11 p., #352466.Research output: Contribution to journal › Article
Open Access
File
Spindle Apparatus
spindles
Masks
stimulated emission
depletion
A call for public archives for biological image data
Ellenberg, J., Swedlow, J. R., Barlow, M., Cook, C. E., Sarkans, U., Patwardhan, A., Brazma, A. & Birney, E., 30 Oct 2018, In : Nature Methods. 15, 11, p. 849-854 6 p.Research output: Contribution to journal › Article
Open Access
File
Bioinformatics
Imaging techniques
Computational Biology
Databases
Technology
An adaptive optics 3D STED microscope for super-resolution imaging of thick samples with background noise suppression using digital image processing
Zdankowski, P., Trusiak, M., Cywinska, M. & Swedlow, J. R., 1 Jan 2018, Speckle 2018: VII International Conference on Speckle Metrology. Jaroszewicz, L. R. & Kujawinska, M. (eds.). Washington: SPIE-International Society for Optical Engineering, Vol. 10834. 9 p. 108342G. (SPIE Proceedings; vol. 10834).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
Noise Suppression
Digital Image Processing
Stimulated emission
Adaptive optics
Super-resolution
3
Citations
(Scopus)
37
Downloads
(Pure)
Killing with proficiency: integrated post-translational regulation of an offensive Type VI secretion system
Ostrowski, A., Cianfanelli, F. R., Porter, M., Mariano, G., Peltier, J., Wong, J. J., Swedlow, J. R., Trost, M. & Coulthurst, S. J., 27 Jul 2018, In : PLoS Pathogens. 14, 7, p. 1-25 25 p., e1007230.Research output: Contribution to journal › Article
Open Access
File
Threonine
Phosphorylation
Serratia marcescens
Proteins
Weapons
Publisher Correction: Image Data Resource: a bioimage data integration and publication platform
Williams, E., Moore, J., Li, S. W., Rustici, G., Tarkowska, A., Chessel, A., Leo, S., Antal, B., Ferguson, R. K., Sarkans, U., Brazma, A., Carazo Salas, R. E. & Swedlow, J. R., 4 Oct 2018, In : Nature Methods. 1 p.Research output: Contribution to journal › Article
Open Access
Data integration
Publications
Licensure
Activities 2010 2010
- 1 Participation in conference
Cancer Forum
Jason R. Swedlow (Invited speaker)5 Mar 2010
Activity: Participating in or organising an event types › Participation in conference
Thesis
Adaptive optics stimulated emission depletion microscope for thick sample imaging
Author: Zdankowski, P., 2018Supervisor: Swedlow, J. (Supervisor) & McGloin, D. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
File
Functional & Molecular Analysis of Bod1in Mitotic Chromosome Segregation
Author: Schleicher, K., 2014Supervisor: Swedlow, J. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
File
The functional and molecular characterisation of the novel DNA damage repair protein Bod1L
Author: Davies, A. M., 2015Supervisor: Swedlow, J. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
File