Owen-Hughes, Tom

Professor

  • United Kingdom

20032025
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Research Output 2003 2019

2019
18 Citations (Scopus)
29 Downloads (Pure)

BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design

Farnaby, W., Koegl, M., Roy, M. J., Whitworth, C., Diers, E., Trainor, N., Zollman, D., Steurer, S., Karolyi-Oezguer, J., Riedmueller, C., Gmaschitz, T., Wachter, J., Dank, C., Galant, M., Sharps, B., Rumpel, K., Traxler, E., Gerstberger, T., Schnitzer, R., Petermann, O. & 17 others, Greb, P., Weinstabl, H., Bader, G., Zoephel, A., Weiss-Puxbaum, A., Ehrenhöfer-Wölfer, K., Wöhrle, S., Boehmelt, G., Rinnenthal, J., Arnhof, H., Wiechens, N., Wu, M-Y., Owen-Hughes, T., Ettmayer, P., Pearson, M., McConnell, D. B. & Ciulli, A., Jul 2019, In : Nature Chemical Biology. 15, 7, p. 672-680 9 p.

Research output: Contribution to journalArticle

Open Access
File
Proteolysis
Adenosine Triphosphatases
Biophysics
Neoplasms
Chromatin Assembly and Disassembly

Publisher Correction: BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design

Farnaby, W., Koegl, M., Roy, M. J., Whitworth, C., Diers, E., Trainor, N., Zollman, D., Steurer, S., Karolyi-oezguer, J., Riedmueller, C., Gmaschitz, T., Wachter, J., Dank, C., Galant, M., Sharps, B., Rumpel, K., Traxler, E., Gerstberger, T., Schnitzer, R., Petermann, O. & 17 others, Greb, P., Weinstabl, H., Bader, G., Zoephel, A., Weiss-Puxbaum, A., Ehrenhöfer-Wölfer, K., Wöhrle, S., Boehmelt, G., Rinnenthal, J., Arnhof, H., Wiechens, N., Wu, M-Y., Owen-Hughes, T., Ettmayer, P., Pearson, M., Mcconnell, D. B. & Ciulli, A., 2 Jul 2019, In : Nature Chemical Biology. 15, 8, p. 846 1 p.

Research output: Contribution to journalArticle

Chromatin Assembly and Disassembly
Ubiquitin-Protein Ligases
Nucleosomes
DNA Repair
Chromatin

The Face of Chromatin Variants

Flaus, A. & Owen-Hughes, T., 5 Sep 2019, In : Cell. 178, 6, p. 1284-1286 3 p.

Research output: Contribution to journalArticle

Chromatin Assembly and Disassembly
Hinges
Histones
Chromatin
Protein Isoforms
2018
1 Citation (Scopus)
98 Downloads (Pure)

Efficient Analysis of Mammalian Polysomes in cells and tissues using Ribo Mega-SEC

Yoshikawa, H., Larance, M., Harney, D. J., Sundaramoorthy, R., Ly, T., Owen-Hughes, T. & Lamond, A. I., 10 Aug 2018, In : eLife. 7, 7, p. 1-26 26 p., e36530.

Research output: Contribution to journalArticle

Open Access
File
Polyribosomes
Tissue
Size exclusion chromatography
Ribosome Subunits
Liver
1 Citation (Scopus)
91 Downloads (Pure)

Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation

Gali, V. K., Dickerson, D., Katou, Y., Fujiki, K., Shirahige, K., Owen-Hughes, T., Kubota, T. & Donaldson, A. D., Nov 2018, In : PLoS Genetics. 14, 11, p. 1-20 20 p., e1007783.

Research output: Contribution to journalArticle

Open Access
File
DNA replication
DNA Replication
Chromatin
chromatin
Proliferating Cell Nuclear Antigen
16 Citations (Scopus)
104 Downloads (Pure)

Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome

Sundaramoorthy, R., Hughes, A., El-Mkami, H., Norman, D. & Owen-Hughes, T., 6 Aug 2018, In : eLife. 2018, 7, p. 1-28 28 p., e35720.

Research output: Contribution to journalArticle

Open Access
File
Chromatin Assembly and Disassembly
Nucleosomes
Chromatin
Histones
DNA
2017
1 Citation (Scopus)
113 Downloads (Pure)

Deciphering Subunit-Specific Functions within SWI/SNF Complexes

Hughes, A. & Owen-Hughes, T., 28 Feb 2017, In : Cell Reports. 18, 9, p. 2075-2076 2 p.

Research output: Contribution to journalComment/debate

Open Access
File
65 Citations (Scopus)
203 Downloads (Pure)

Histone degradation in response to DNA damage enhances chromatin dynamics and recombination rates

Hauer, M. H., Seeber, A., Singh, V., Thierry, R., Sack, R., Amitai, A., Kryzhanovska, M., Eglinger, J., Holcman, D., Owen-Hughes, T. & Gasser, S. M., Feb 2017, In : Nature Structural & Molecular Biology. 24, 2, p. 99-107 13 p.

Research output: Contribution to journalArticle

Open Access
File
Histones
Genetic Recombination
DNA Damage
Chromatin
Nucleosomes
26 Citations (Scopus)
124 Downloads (Pure)

Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes

Sundaramoorthy, R., Hughes, A. L., Singh, V., Wiechens, N., Ryan, D. P., El-Mkami, H., Petoukhov, M., Svergun, D. I., Treutlein, B., Quack, S., Fischer, M., Michaelis, J., Bottcher, B., Norman, D. G. & Owen-Hughes, T., 13 Apr 2017, In : eLife. 6, 28 p., e22510.

Research output: Contribution to journalArticle

Open Access
File
Chromatin Assembly and Disassembly
Nucleosomes
Chromatin
DNA
Enzymes
3 Citations (Scopus)

Unlocking the nucleosome

Flaus, A. & Owen-Hughes, T., 20 Jan 2017, In : Science. 355, 6322, p. 245-246 2 p.

Research output: Contribution to journalArticle

Nucleosomes
Histones
Genome
Chromatin Assembly and Disassembly
DNA
2016
25 Citations (Scopus)
142 Downloads (Pure)

Establishment of a promoter based chromatin architecture on recently replicated DNA can accommodate variable internucleosome spacing

Fennessy, R. T. & Owen-Hughes, T., 6 Sep 2016, In : Nucleic Acids Research. 44, 15, p. 7189-7203 15 p.

Research output: Contribution to journalArticle

Open Access
File
Nucleosomes
Chromatin
DNA
DNA Replication
Chromatin Assembly and Disassembly
6 Citations (Scopus)
149 Downloads (Pure)
Open Access
File
Cell Division
Chromatin
Genes
Workflow
Eukaryota
174 Citations (Scopus)
159 Downloads (Pure)

How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?

Schurch, N. J., Schofield, P., Gierliński, M., Cole, C., Sherstnev, A., Singh, V., Wrobel, N., Gharbi, K., Simpson, G. G., Owen-Hughes, T., Blaxter, M. & Barton, G. J., Jun 2016, In : RNA: a Publication of the RNA Society. 22, 6, p. 839-851 13 p.

Research output: Contribution to journalArticle

Open Access
File
RNA
Genes
Research Design
Genome
Guidelines
28 Citations (Scopus)
126 Downloads (Pure)

The chromatin remodelling enzymes SNF2H and SNF2L position nucleosomes adjacent to CTCF and other transcription

Wiechens, N., Singh, V., Gkikopoulos, T., Schofield, P., Rocha, S. & Owen-Hughes, T., 28 Mar 2016, In : PLoS Genetics.

Research output: Contribution to journalArticle

Open Access
File
nucleosomes
Chromatin Assembly and Disassembly
Nucleosomes
chromatin
binding sites
16 Citations (Scopus)
215 Downloads (Pure)

The histone chaperone Vps75 forms multiple oligomeric assemblies capable of mediating exchange between histone H3–H4 tetramers and Asf1–H3–H4 complexes

Hammond, C. M., Sundaramoorthy, R., Larance, M., Lamond, A., Stevens, M. A., El-Mkami, H., Norman, D. & Owen-Hughes, T., 27 Jul 2016, In : Nucleic Acids Research. 44, 13, p. 6157-6172 16 p.

Research output: Contribution to journalArticle

Open Access
File
Histone Chaperones
Histones
Electrons
Structural Models
X Ray Crystallography
2015
30 Citations (Scopus)
129 Downloads (Pure)

Statistical models for RNA-seq data derived from a two-condition 48-replicate experiment

Gierlinski, M., Cole, C., Schofield, P., Schurch, N. J., Sherstnev, A., Singh, V., Wrobel, N., Gharbi, K., Simpson, G., Owen-Hughes, T., Blaxter, M. & Barton, G. J., 15 Nov 2015, In : Bioinformatics. 31, 22, p. 3625-3630 6 p.

Research output: Contribution to journalArticle

Open Access
File
2014
57 Citations (Scopus)
320 Downloads (Pure)

Acetylation of histone H3 at lysine 64 regulates nucleosome dynamics and facilitates transcription

Di Cerbo, V., Mohn, F., Ryan, D. P., Montellier, E., Kacem, S., Tropberger, P., Kallis, E., Holzner, M., Hoerner, L., Feldmann, A., Richter, F. M., Bannister, A. J., Mittler, G., Michaelis, J., Khochbin, S., Feil, R., Schuebeler, D., Owen-Hughes, T., Daujat, S. & Schneider, R., 25 Mar 2014, In : eLife. 3, e01632.

Research output: Contribution to journalArticle

Open Access
File
Acetylation
Nucleosomes
Transcription
Histones
Lysine
3 Citations (Scopus)
150 Downloads (Pure)
Open Access
File
Distance measurement
Hordeolum
Histone Chaperones
Electrons
Structural Models
24 Citations (Scopus)
181 Downloads (Pure)

The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution

Bowman, A., Hammond, C. M., Stirling, A., Ward, R., Shang, W., El-Mkami, H., Robinson, D. A., Svergun, D. I., Norman, D. G. & Owen-Hughes, T., 2014, In : Nucleic Acids Research. 42, 9, p. 6038-6051 13 p.

Research output: Contribution to journalArticle

Open Access
File
Histone Chaperones
Histones
Electrons
Saccharomycetales
Nucleosomes
9 Citations (Scopus)
138 Downloads (Pure)

The spatial effect of protein deuteration on nitroxide spin-label relaxation: implications for EPR distance measurement

El Mkami, H., Ward, R., Bowman, A., Owen-Hughes, T. & Norman, D. G., Nov 2014, In : Journal of Magnetic Resonance . 248, p. 36-41 6 p.

Research output: Contribution to journalArticle

Open Access
File
Spin Labels
Distance measurement
Paramagnetic resonance
Electrons
proteins
2013
3 Citations (Scopus)

A comparison of in vitro nucleosome positioning mapped with chicken, frog and a variety of yeast core histones

Allan, J., Fraser, R. M., Owen-Hughes, T., Docherty, K. & Singh, V., 15 Nov 2013, In : Journal of Molecular Biology. 425, 22, p. 4206-4222 17 p.

Research output: Contribution to journalArticle

Nucleosomes
Anura
Histones
Chickens
Yeasts
37 Citations (Scopus)

CENP-A confers a reduction in height on octameric nucleosomes

Miell, M. D. D., Barysz, H. M., Rappsilber, J., Allshire, R. C., Fuller, C. J., Straight, A. F., Guse, A., Downes, A. & Owen-Hughes, T., 2013, In : Nature Structural & Molecular Biology.

Research output: Contribution to journalArticle

Nucleosomes
Kinetochores
Histones
Drosophila
centromere protein A
277 Citations (Scopus)

Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes

Narlikar, G. J., Sundaramoorthy, R. & Owen-Hughes, T., 2013, In : Cell. 154, 3, p. 490-503 14 p.

Research output: Contribution to journalArticle

Chromatin Assembly and Disassembly
Chromatin
Adenosine Triphosphate
Enzymes
Genes
49 Citations (Scopus)

PICH: A DNA Translocase Specially Adapted for Processing Anaphase Bridge DNA

Biebricher, A., Hirano, S., Enzlin, J. H., Wiechens, N., Streicher, W. W., Huttner, D., Wang, L. H. C., Nigg, E. A., Owen-Hughes, T., Liu, Y., Peterman, E., Wuite, G. J. L. & Hickson, I. D., 12 Sep 2013, In : Molecular Cell. 51, 5, p. 691-701 11 p.

Research output: Contribution to journalArticle

DNA Helicases
Anaphase
DNA
Optical Tweezers
Nucleic Acid Denaturation
37 Citations (Scopus)

Structural plasticity of histones H3–H4 facilitates their allosteric exchange between RbAp48 and ASF1

Zhang, W., Yyl, M., Ward, R., Sobott, F., Maman, J., Murthy, A., Watson, A., Fedorov, O., Bowman, A., Owen-Hughes, T., El Mkami, H., Murzina, N., Norman, D. G. & Laue, E., Jan 2013, In : Nature Structural & Molecular Biology. 20, 1, p. 29-35 7 p.

Research output: Contribution to journalArticle

Histones
Histone Chaperones
Proteins
Nucleosomes
DNA Replication
2012

Evolutionary insights into genome-wide nucleosome positioning

Singh, V. & Owen-Hughes, T., 27 Sep 2012, In : Genome Biology. 13, 9, p. 1-3 3 p., 170.

Research output: Contribution to journalComment/debate

Open Access
nucleosomes
Trans-Activators
Nucleosomes
positioning
genome
16 Citations (Scopus)

Making sense of transcribing chromatin

Owen-Hughes, T. & Gkikopoulos, T., 1 Jan 2012, In : Current Opinion in Cell Biology.

Research output: Contribution to journalArticle

Nucleosomes
Chromatin
Eukaryota
DNA
Genome
50 Citations (Scopus)
188 Downloads (Pure)

Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping

Allan, J., Fraser, R. M., Owen-Hughes, T. & Keszenman-Pereyra, D., 30 Mar 2012, In : Journal of Molecular Biology. 417, 3, p. 152-164 13 p.

Research output: Contribution to journalArticle

File
3 Citations (Scopus)

Sulfyhydryl-reactive site-directed cross-linking as a method for probing the tetrameric structure of histones H3 and H4

Bowman, A. & Owen-Hughes, T., 2012, Chromatin remodeling: methods and protocols. Morse, R. H. (ed.). Humana Press, p. 373-387 15 p. (Methods in Molecular Biology; vol. 833).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Histones
Catalytic Domain
Proteins
Chromatin
Histone Chaperones
42 Citations (Scopus)

The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4

Watson, A. A., Mahajan, P., Mertens, H. D. T., Deery, M. J., Zhang, W., Chen, Y., Edlich, C., Laue, E. D., Berridge, G., Burgess-Brown, N. A., Gileadi, O., Svergun, D. I., Pham, P., Du, X., Bartke, T., Kouzarides, T., Wiechens, N. & Owen-Hughes, T., 2012, In : Journal of Molecular Biology. 422, 1, p. 3-17 15 p.

Research output: Contribution to journalArticle

Mi-2 Nucleosome Remodeling and Deacetylase Complex
Nucleosomes
Human Activities
Chromatin
Adenosine Triphosphatases
2011
171 Citations (Scopus)

A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization

Gkikopoulos, T., Schofield, P., Singh, V., Pinskaya, M., Mellor, J., Smolle, M., Workman, J. L., Barton, G. J. & Owen-Hughes, T., 23 Sep 2011, In : Science. 333, 6050, p. 1758-1760 3 p.

Research output: Contribution to journalArticle

87 Citations (Scopus)

DNA repair factor APLF is a histone chaperone

Mehrotra, P. V., Ahel, D., Ryan, D. P., Weston, R., Wiechens, N., Kraehenbuehl, R., Owen-Hughes, T. & Ahel, I., 7 Jan 2011, In : Molecular Cell. 41, 1, p. 46-55 10 p.

Research output: Contribution to journalArticle

66 Citations (Scopus)

Mechanisms for ATP-dependent chromatin remodelling: the means to the end

Flaus, A. & Owen-Hughes, T., Oct 2011, In : FEBS Journal. 278, 19, p. 3579-3595 17 p.

Research output: Contribution to journalReview article

56 Citations (Scopus)

Snf2-family proteins: chromatin remodellers for any occasion

Ryan, D. P. & Owen-Hughes, T., Oct 2011, In : Current Opinion in Chemical Biology. 15, 5, p. 649-656 8 p.

Research output: Contribution to journalReview article

71 Citations (Scopus)
103 Downloads (Pure)

The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains

Ryan, D. P., Sundaramoorthy, R., Martin, D., Singh, V. & Owen-Hughes, T., 6 Jul 2011, In : EMBO Journal. 30, 13, p. 2596-2609 14 p.

Research output: Contribution to journalArticle

Open Access
File
56 Citations (Scopus)
161 Downloads (Pure)

The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation

Bowman, A., Ward, R., Wiechens, N., Singh, V., El-Mkami, H., Norman, D. G. & Owen-Hughes, T., 18 Feb 2011, In : Molecular Cell. 41, 4, p. 398-408 11 p.

Research output: Contribution to journalArticle

File
33 Citations (Scopus)
112 Downloads (Pure)

The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4

Gkikopoulos, T., Singh, V., Tsui, K., Awad, S., Renshaw, M. J., Scholfield, P., Barton, G. J., Nislow, C., Tanaka, T. U. & Owen-Hughes, T., 18 May 2011, In : EMBO Journal. 30, 10, p. 1919-1927 9 p.

Research output: Contribution to journalArticle

Open Access
File
2010
10 Citations (Scopus)

ATRX: put me on repeat

Whitehouse, I. & Owen-Hughes, T., 29 Oct 2010, In : Cell. 143, 3, p. 335-336 2 p.

Research output: Contribution to journalEditorial

93 Citations (Scopus)

EPR distance measurements in deuterated proteins

Ward, R., Bowman, A., Sozudogru, E., El-Mkami, H., Owen-Hughes, T. & Norman, D. G., Nov 2010, In : Journal of Magnetic Resonance Imaging. 207, 1, p. 164-167 4 p.

Research output: Contribution to journalArticle

31 Citations (Scopus)
113 Downloads (Pure)

Probing the (H3-H4)(2) histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling

Bowman, A., Ward, R., El-Mkami, H., Owen-Hughes, T. & Norman, D. G., Jan 2010, In : Nucleic Acids Research. 38, 2, p. 695-707 13 p.

Research output: Contribution to journalArticle

Open Access
File
42 Citations (Scopus)
124 Downloads (Pure)

The Snf2 Homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme

Awad, S., Ryan, D., Prochasson, P., Owen-Hughes, T. & Hassan, A. H., 26 Mar 2010, In : Journal of Biological Chemistry. 285, 13, p. 9477-9484 8 p.

Research output: Contribution to journalArticle

Open Access
File
2009
16 Citations (Scopus)

ABF1-binding sites promote efficient global genome nucleotide excision repair

Yu, S., Smirnova, J. B., Friedberg, E. C., Stillman, B., Akiyama, M., Owen-Hughes, T., Waters, R. & Reed, S. H., 9 Jan 2009, In : Journal of Biological Chemistry. 284, 2, p. 966-973 8 p.

Research output: Contribution to journalArticle

306 Citations (Scopus)

A method for genetically installing site-specific acetylation in recombinant histories defines the effects of H3 K56 acetylation

Neumann, H., Hancock, S. M., Buning, R., Routh, A., Chapman, L., Somers, J., Owen-Hughes, T., van Noort, J., Rhodes, D. & Chin, J. W., 9 Oct 2009, In : Molecular Cell. 36, 1, p. 153-163 11 p.

Research output: Contribution to journalArticle

64 Citations (Scopus)

FACT facilitates chromatin transcription by RNA polymerases I and III

Birch, J. L., Tan, B. C-M., Panov, K. I., Panova, T. B., Andersen, J. S., Owen-Hughes, T. A., Russell, J., Lee, S-C. & Zomerdijk, J. C. B. M., 8 Apr 2009, In : EMBO Journal. 28, 7, p. 854-865 12 p.

Research output: Contribution to journalArticle

24 Citations (Scopus)

Long distance PELDOR measurements on the histone core particle

Ward, R., Bowman, A., El-Mkami, H., Owen-Hughes, T. & Norman, D. G., 4 Feb 2009, In : Journal of the American Chemical Society. 131, 4, p. 1348-+ 3 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)
199 Downloads (Pure)

Mutations to the histone H3 alpha N region selectively alter the outcome of ATP-dependent nucleosome-remodelling reactions

Somers, J. & Owen-Hughes, T., May 2009, In : Nucleic Acids Research. 37, 8, p. 2504-2513 10 p.

Research output: Contribution to journalArticle

Open Access
File
70 Citations (Scopus)

Nucleosomes can invade DNA territories occupied by their neighbors

Engeholm, M., de Jager, M., Flaus, A., Brenk, R., van Noort, J. & Owen-Hughes, T., Feb 2009, In : Nature Structural & Molecular Biology. 16, 2, p. 151-158 8 p.

Research output: Contribution to journalArticle

73 Citations (Scopus)

Phosphorylation of histone H3 Thr-45 is linked to apoptosis

Hurd, P. J., Bannister, A. J., Halls, K., Dawson, M. A., Vermeulen, M., Olsen, J. V., Ismail, H., Somers, J., Mann, M., Owen-Hughes, T., Gout, I. & Kouzarides, T., 12 Jun 2009, In : Journal of Biological Chemistry. 284, 24, p. 16575-16583 9 p.

Research output: Contribution to journalArticle

334 Citations (Scopus)

Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1

Ahel, D., Horejsi, Z., Wiechens, N., Polo, S. E., Garcia-Wilson, E., Ahel, I., Flynn, H., Skehel, M., West, S. C., Jackson, S. P., Owen-Hughes, T. & Boulton, S. J., 4 Sep 2009, In : Science. 325, 5945, p. 1240-1243 4 p.

Research output: Contribution to journalArticle

25 Citations (Scopus)

SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter

Gkikopoulos, T., Havas, K. M., Dewar, H. & Owen-Hughes, T., Aug 2009, In : Molecular and Cellular Biology. 29, 15, p. 4057-4066 10 p.

Research output: Contribution to journalArticle