Biochemical Analysis of the PINK-1 Parkin Signalling Pathway in Parkinson's Disease (Senior Clinical Fellowship)

Research output

Research output per year 2013 2014 2015 2023 2024 2025

• 24 Article
• 2 Review article
• 1 Preprint

Research output per year

Research output per year

Search results

• 2025

  Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain

  Bagnoli, E., Lin, Y.-E., Burel, S., Jaimon, E., Antico, O., Themistokleous, C., Nikoloff, J. M., Squires, S., Morella, I., Watzlawik, J. O., Fiesel, F. C., Springer, W., Tonelli, F., Lis, P., Brooks, S. P., Dunnett, S. B., Brambilla, R., Alessi, D. R., Pfeffer, S. R. & Muqit, M. M. K. (Lead / Corresponding author), 4 Feb 2025, In: Proceedings of the National Academy of Sciences of the United States of America. 122, 5, 12 p., e2412029122.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Neuroprotective 100%
  • Leucine-rich Repeat Kinase 2 (LRRK2) 100%
  • Ciliogenesis 100%
  • PTEN-induced Kinase 1 (PINK1) 100%
  • Phosphotransferase 100%
  1 Citation (Scopus)

  19 Downloads (Pure)
• 2024

  Additional feedforward mechanism of Parkin activation via binding of phospho-UBL and RING0 in trans

  Lenka, D. R., Dahe, S. V., Antico, O., Sahoo, P., Prescott, A. R., Muqit, M. M. K. & Kumar, A. (Lead / Corresponding author), 2 Sept 2024, (E-pub ahead of print) In: eLife. 13, 31 p., RP96699.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Parkin 100%
  • Feed-forward Mechanism 100%
  • Ubiquitin 100%
  • Crystal Structure 25%
  • Phosphotransferase 25%
  19 Downloads (Pure)

• CURTAIN – A unique web-based tool for exploration and sharing of MS-based proteomics data

  Phung, T. K. (Lead / Corresponding author), Berndsen, K., Shastry, R., Phan, T. L. C. H. B., Muqit, M. M. K., Alessi, D. R. (Lead / Corresponding author) & Nirujogi, R. S. (Lead / Corresponding author), 13 Feb 2024, In: Proceedings of the National Academy of Sciences. 121, 7, 12 p., e2312376121.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • HTTPS 100%
  • Mass Spectrometry Analysis 100%
  • Web Tool 100%
  • Proteomics Data 100%
  • Mass Spectrometry 100%
  3 Citations (Scopus)

  161 Downloads (Pure)

• Design and high-throughput implementation of MALDI-TOF/MS-based assays for Parkin E3 ligase activity

  Traynor, R., Moran, J., Stevens, M., Antico, O., Knebel, A., Behrouz, B., Merchant, K., Hastie, C. J., Davies, P., Muqit, M. M. K. & De Cesare, V. (Lead / Corresponding author), 26 Feb 2024, In: Cell Reports Methods. 4, 2, 16 p., 100712.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • High-throughput 100%
  • E3 Ligase Activity 100%
  • Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS) 100%
  • Parkin 100%
  • Ligase 100%
  2 Citations (Scopus)

  78 Downloads (Pure)

• Development and characterization of phospho-ubiquitin antibodies to monitor PINK1-PRKN signaling in cells and tissue

  Watzlawik, J. O., Hou, X., Richardson, T., Lewicki, S. L., Siuda, J., Wszolek, Z. K., Cook, C. N., Petrucelli, L., DeTure, M., Dickson, D. W., Antico, O., Muqit, M. M. K., Fishman, J. B., Pirani, K., Kumaran, R., Polinski, N. K., Fiesel, F. C. & Springer, W. (Lead / Corresponding author), 2024, In: Autophagy. 20, 9, p. 2076-2091 16 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Ubiquitin 100%
  • Phospho-ubiquitin 100%
  • PINK1-PRKN 100%
  • Mouse 100%
  • Peripheral Blood Mononuclear Cell 100%
  5 Citations (Scopus)

  75 Downloads (Pure)

• Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain

  Bagnoli, E., Lin, Y. E., Burel, S., Jaimon, E., Antico, O., Themistokleous, C., Nikolof, J. M., Morella, I., Watzlawik, J. O., Fiesel, F. C., Springer, W., Tonelli, F., Brooks, S. P., Dunnett, S. B., Brambilla, R., Alessi, D., Pfeffer, S. R. & Muqit, M. (Lead / Corresponding author), 11 Jun 2024, BioRxiv, 35 p.

  Research output: Working paper/Preprint › Preprint

  • Neuroprotective Agent 100%
  • Parkinson's Disease 100%
  • Neuroprotective 100%
  • Leucine-rich Repeat Kinase 2 (LRRK2) 100%
  • Ciliogenesis 100%

• Mechanism of human PINK1 activation at the TOM complex in a reconstituted system

  Raimi, O. G., Ojha, H., Ehses, K., Dederer, V., Lange, S. M., Rivera, C. P., Deegan, T. D., Chen, Y., Wightman, M., Toth, R., Labib, K. P. M., Mathea, S., Ranson, N., Fernández-Busnadiego, R. & Muqit, M. M. K. (Lead / Corresponding author), Jun 2024, In: Science Advances. 10, 23, 16 p., eadn7191.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • PTEN-induced Kinase 1 (PINK1) 100%
  • Reconstituted System 100%
  • Outer Membrane Complex 100%
  • Phosphotransferase 100%
  • Kinase 100%
  11 Citations (Scopus)

  100 Downloads (Pure)
• 2023

  Role of autophagy pathway in Parkinson's disease and related Genetic Neurological disorders

  Themistokleous, C., Bagnoli, E., Parulekar, R. & M K Muqit, M. (Lead / Corresponding author), 15 Jun 2023, In: Journal of Molecular Biology. 435, 12, 26 p., 168144.

  Research output: Contribution to journal › Review article › peer-review

  Open Access

  File

  • Parkinson's Disease 100%
  • Autophagy 100%
  • Autophagy Pathway 100%
  • Genetic Neurological Disorders 100%
  • Nervous System Disorder 100%
  19 Citations (Scopus)

  197 Downloads (Pure)

• Structure-based design and characterization of Parkin-activating mutations

  Stevens, M. U., Croteau, N., Eldeeb, M. A., Antico, O., Zeng, Z. W., Toth, R., Durcan, T. M., Springer, W., Fon, E. A. (Lead / Corresponding author), Muqit, M. M. K. (Lead / Corresponding author) & Trempe, J.-F. (Lead / Corresponding author), Jun 2023, In: Life Science Alliance. 6, 6, 16 p., e202201419.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • RING1 100%
  • Structure-based Design 100%
  • Activating mutation 100%
  • Parkin 100%
  • Small Molecule 50%
  21 Citations (Scopus)

  128 Downloads (Pure)

• Whole proteome copy number dataset in primary mouse cortical neurons

  Antico, O., Nirujogi, R. S. & Muqit, M. M. K. (Lead / Corresponding author), Aug 2023, In: Data in Brief. 49, 11 p., 109336.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Copy number 100%
  • Single-cell Proteomics 100%
  • Mouse Cortical Neurons 100%
  • Data-dependent Acquisition 100%
  • Data-independent Acquisition 100%
  2 Citations (Scopus)

  130 Downloads (Pure)
• 2022

  Mapping of a N-terminal α-helix domain required for human PINK1 stabilization, Serine228 autophosphorylation and activation in cells

  Kakade, P., Ojha, H., Raimi, O. G., Shaw, A., Waddell, A. D., Ault, J. R., Burel, S., Brockmann, K., Kumar, A., Ahangar, M. S., Krysztofinska, E. M., Macartney, T., Bayliss, R., Fitzgerald, J. C. & Muqit, M. M. K. (Lead / Corresponding author), Jan 2022, In: Open Biology. 12, 1, p. 1-16 16 p., 210264.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Autophosphorylation 100%
  • N-terminal Helix 100%
  • C-terminal Extension 100%
  • N-Terminus 100%
  • PINK1 100%
  22 Citations (Scopus)

  173 Downloads (Pure)

• PTEN-induced kinase 1 (PINK1) and Parkin: Unlocking a mitochondrial quality control pathway linked to Parkinson's disease

  Agarwal, S. & Muqit, M. M. K. (Lead / Corresponding author), Feb 2022, In: Current Opinion in Neurobiology. 72, p. 111-119 9 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Parkinson's Disease 100%
  • PTEN-induced Kinase 1 (PINK1) 100%
  • Parkin 100%
  • Mitochondrial Quality Control 100%
  • Phosphotransferase 100%
  58 Citations (Scopus)

  285 Downloads (Pure)
• 2021

  Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1

  Antico, O., Ordureau, A., Stevens, M., Singh, F., Nirujogi, R. S., Gierlinski, M., Barini, E., Rickwood, M. L., Prescott, A., Toth, R., Ganley, I. G., Harper, J. W. (Lead / Corresponding author) & Muqit, M. M. K. (Lead / Corresponding author), 12 Nov 2021, In: Science Advances. 7, 46, p. 1-21 21 p., eabj0722.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mitochondria 100%
  • Primary Neurons 100%
  • Ubiquitylation 100%
  • Parkin 100%
  • Target Following 100%
  26 Citations (Scopus)

  387 Downloads (Pure)
• 2020

  Parkinson’s: A Disease of Aberrant Vesicle Trafficking

  Singh, P. K. & Muqit, M. M. K. (Lead / Corresponding author), Oct 2020, In: Annual Review of Cell and Developmental Biology. 36, p. 237-264 28 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Parkinson 100%
  • Parkinson's Disease 100%
  • Vesicle Trafficking 100%
  • Phosphotransferase 100%
  • Kinase 100%
  62 Citations (Scopus)

  209 Downloads (Pure)

• PINK1-dependent phosphorylation of Serine111 within the SF3 motif of Rab GTPases impairs effector interactions and LRRK2 mediated phosphorylation at Threonine72

  Vieweg, S., Mulholland, K., Brauning, B., Kacharia, N., Lai, Y.-C., Toth, R., Singh, P. K., Volpi, I., Sattler, M., Groll, M., Itzen, A. & Muqit, M. M. K. (Lead / Corresponding author), 11 May 2020, In: Biochemical Journal. 477, 9, p. 1651-1668 18 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Phosphorylation 100%
  • Leucine-rich Repeat Kinase 2 (LRRK2) 100%
  • Rab GTPase 100%
  • PTEN-induced Kinase 1 (PINK1) 100%
  • Effectors Interaction 100%
  24 Citations (Scopus)

  120 Downloads (Pure)
• 2019

  Are PARKIN patients ideal candidates for dopaminergic cell replacement therapies?

  Kunath, T. (Lead / Corresponding author), Natalwala, A., Chan, C., Chen, Y., Stecher, B., Taylor, M., Khan, S. & Muqit, M. M. K., 25 Feb 2019, In: European Journal of Neuroscience. 49, 4, p. 453-462 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Dopaminergic Neurons 100%
  • Parkin 100%
  • Cell Replacement Therapy 100%
  • Dopaminergic 100%
  • Synucleinopathy 66%
  14 Citations (Scopus)

  207 Downloads (Pure)

• Therapeutic approaches to enhance PINK1/Parkin mediated mitophagy for the treatment of Parkinson's disease

  Miller, S. (Lead / Corresponding author) & Muqit, M. M. K., 13 Jul 2019, In: Neuroscience Letters. 705, p. 7-13 7 p.

  Research output: Contribution to journal › Review article › peer-review

  Open Access

  File

  • Parkinson's Disease 100%
  • Therapeutic Approaches 100%
  • Mitophagy 100%
  • PINK1-Parkin Pathway 100%
  • Parkin-mediated Mitophagy 100%
  104 Citations (Scopus)

  329 Downloads (Pure)
• 2018

  Phosphorylation of Parkin at Serine65 is essential for its activation in vivo

  McWilliams, T. G. (Lead / Corresponding author), Barini, E., Pohjolan-Pirhonen, R., Brooks, S. P., Singh, F., Burel, S., Balk, K., Kumar, A., Montava Garriga, L., Prescott, A., Hassoun, S. M., Mouton-Liger, F., Ball, G., Hills, R., Knebel, A., Ulusoy, A., Di Monte, D. A., Tamjar, J., Antico, O. & Fears, K. & 11 others, Smith, L., Brambilla, R., Palin, E., Valori, M., Eerola-Rautio, J., Tienari, P., Corti, O., Dunnett, S. B., Ganley, I. G., Suomalainen, A. & Muqit, M. M. K. (Lead / Corresponding author), Nov 2018, In: Open Biology. 8, 11, 18 p., 181108.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Phosphorylation 100%
  • Parkin 100%
  • In Vivo 100%
  • Serine 100%
  • PINK1 80%
  85 Citations (Scopus)

  317 Downloads (Pure)

• The Anthelmintic Drug Niclosamide and Its Analogues Activate the Parkinson's Disease Associated Protein Kinase PINK1

  Barini, E., Miccoli, A., Tinarelli, F., Mulholland, K., Kadri, H., Khanim, F., Stojanovski, L., Read, K. D., Burness, K., Blow, J. J., Mehellou, Y. (Lead / Corresponding author) & Muqit, M. M. K. (Lead / Corresponding author), 2 Mar 2018, In: ChemBioChem. 19, 5, p. 425-429 5 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Parkinson's Disease 100%
  • Protein Kinase 100%
  • Disease-related Proteins 100%
  • Anthelmintic Drugs 100%
  • Niclosamide 100%
  43 Citations (Scopus)

  240 Downloads (Pure)
• 2015

  A versatile strategy for the semisynthetic production of Ser65 phosphorylated ubiquitin and its biochemical and structural characterisation

  Han, C., Pao, K.-C., Kazlauskaite, A., Muqit, M. M. K. & Virdee, S. (Lead / Corresponding author), 27 Jul 2015, In: ChemBioChem. 16, 11, p. 1574-1579 6 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Ubiquitin 100%
  • Biochemical Characterization 100%
  • Semi-synthetic 100%
  • Structural Characterization 100%
  • Phosphorylated Ubiquitin 100%
  5 Citations (Scopus)

• Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation

  Kazlauskaite, A., Martínez-Torres, R. J., Wilkie, S., Kumar, A., Peltier, J., Gonzalez, A., Johnson, C., Zhang, J., Hope, A. G., Peggie, M., Trost, M., van Aalten, D. M., Alessi, D. R., Prescott, A. R., Knebel, A., Walden, H. & Muqit, M. M. (Lead / Corresponding author), 1 Aug 2015, In: EMBO Reports. 16, 8, p. 939-954 16 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Phosphorylation 100%
  • Parkin 100%
  • Phosphorylated Ubiquitin 100%
  • Serine 100%
  • Ubiquitin 100%
  190 Citations (Scopus)

  283 Downloads (Pure)

• Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog

  Rogerson, D. T., Sachdeva, A., Wang, K., Haq, T., Kazlauskaite, A., Hancock, S. M., Huguenin-Dezot, N., Muqit, M. M. K., Fry, A. M., Bayliss, R. & Chin, J. W. (Lead / Corresponding author), Jul 2015, In: Nature Chemical Biology. 11, 7, p. 496-503 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Phosphorylation 100%
  • Phosphoserine 100%
  • Nonhydrolyzable Analog 100%
  • Genetic Encoding 100%
  • Genetics 100%
  189 Citations (Scopus)

• Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1

  Lai, Y.-C., Kondapalli, C., Lehneck, R., Procter, J. B., Dill, B. D., Woodroof, H. I., Gourlay, R., Peggie, M., Macartney, T. J., Corti, O., Corvol, J.-C., Campbell, D. G., Itzen, A., Trost, M. (Lead / Corresponding author) & Muqit, M. M. (Lead / Corresponding author), 12 Nov 2015, In: EMBO Journal. 34, 22, p. 2840-2861 22 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Downstream Targets 100%
  • Phosphoproteomics 100%
  • Rab GTPase 100%
  • PTEN-induced Kinase 1 (PINK1) 100%
  • Phosphotransferase 100%
  142 Citations (Scopus)

  160 Downloads (Pure)
• 2014

  Parkin is activated by PINK1-dependent phosphorylation of ubiquitin at Serine⁶⁵

  Kazlauskaite, A. (Lead / Corresponding author), Kondapalli, C., Gourlay, R., Campbell, D. G., Ritorto, M. S., Hofmann, K., Alessi, D. R., Knebel, A., Trost, M. & Muqit, M. M. K. (Lead / Corresponding author), 15 May 2014, In: Biochemical Journal. 460, 1, p. 127-139 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Phosphorylation 100%
  • Ubiquitin 100%
  • PTEN-induced Kinase 1 (PINK1) 100%
  • Parkin 100%
  • Phosphotransferase 100%
  649 Citations (Scopus)

  227 Downloads (Pure)

• Phosphorylation of Parkin at Serine65 is essential for activation: elaboration of a Miro1 substrate-based assay of Parkin E3 ligase activity

  Kazlauskaite, A., Kelly, V., Johnson, C., Baillie, C., Hastie, C. J., Peggie, M., Macartney, T., Woodroof, H. I., Alessi, D. R., Pedrioli, P. G. A. & Muqit, M. M. K., 19 Mar 2014, In: Open Biology. 4, 14 p., 130213.

  Research output: Contribution to journal › Article › peer-review

  • Phosphorylation 100%
  • E3 Ligase Activity 100%
  • Parkin 100%
  • Miro1 100%
  • Ubiquitin 100%
  110 Citations (Scopus)

• The novel MAPT mutation K298E: mechanisms of mutant tau toxicity, brain pathology and tau expression in induced fibroblast-derived neurons

  Iovino, M., Pfisterer, U., Holton, J. L., Lashley, T., Swingler, R. J., Calo, L., Treacy, R., Revesz, T., Parmar, M., Goedert, M., Muqit, M. M. K. (Lead / Corresponding author) & Spillantini, M. G. (Lead / Corresponding author), Feb 2014, In: Acta Neuropathologica. 127, 2, p. 283-295

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Fibroblasts 100%
  • Microtubules 100%
  • Brain Pathology 100%
  • Tau Toxicity 100%
  • Tau mutation 100%
  30 Citations (Scopus)

  303 Downloads (Pure)
• 2013

  TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency

  Flinn, L. J., Keatinge, M., Bretaud, S., Mortiboys, H., Matsui, H., De Felice, E., Woodroof, H. I., Brown, L., McTighe, A., Soellner, R., Allen, C. E., Heath, P. R., Milo, M., Muqit, M. M. K., Reichert, A. S., Köster, R. W., Ingham, P. W. & Bandmann, O. (Lead / Corresponding author), Dec 2013, In: Annals of Neurology. 74, 6, p. 837-847 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mitochondrial Dysfunction 100%
  • Neuronal Loss 100%
  • Dopaminergic Neurons 100%
  • PINK1 100%
  • Mitochondrial Disorder 100%
  70 Citations (Scopus)

  192 Downloads (Pure)