β-Adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia

A. Collett, S. J. Ramminger, R. E. Olver, S. M. Wilson

    Research output: Contribution to journalArticlepeer-review

    70 Citations (Scopus)

    Abstract

    Distal lung epithelial cells isolated from fetal rats were cultured (48 h) on permeable supports so that transepithelial ion transport could be quantified electrometrically. Unstimulated cells generated a short-circuit current (I sc) that was inhibited (~80%) by apical amiloride. The current is thus due, predominantly, to the absorption of Na+ from the apical solution. Isoprenaline increased the amiloride-sensitive I sc about twofold. Experiments in which apical membrane Na+ currents were monitored in basolaterally permeabilized cells showed that this was accompanied by a rise in apical Na+ conductance (G Na+). Isoprenaline also increased apical Cl- conductance (G Cl-) by activating an anion channel species sensitive to glibenclamide but unaffected by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The isoprenaline-evoked changes inG Na+ and G Cl-could account for the changes in I sc observed in intact cells. Glibenclamide had no effect upon the isoprenaline-evoked stimulation of I sc orG Na+ demonstrating that the rise inG Cl- is not essential to the stimulation of Na+ transport.
    Original languageEnglish
    Pages (from-to)621-630
    Number of pages10
    JournalAmerican Journal of Physiology: Lung Cellular and Molecular Physiology
    Volume282
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Alveolar ion transport
    • Ussing chambers
    • Permeabilized epithelia

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