σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh

Ewan J. Murray, Mark A. Strauch, Nicola R. Stanley-Wall (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription from these operons is repressed by two key regulators, AbrB and SinR. Relief of inhibition is necessary to allow biofilm formation to proceed. Here we present data indicating that Abh, a sequence and structural homologue of AbrB, regulates biofilm architecture by B. subtilis when colony morphology and pellicle formation are assessed. Data indicating that abh expression is dependent on the environmental signals that stimulate the activity of the extracytoplasmic function sigma-factor sigma(X) are shown. We demonstrate that expression of slrR, the proposed activator of yqxM transcription, is positively controlled by Abh. Furthermore, Abh is shown to activate transcription from the promoter of the eps operon through its control of SlrR. These findings add to the increasingly complex transcriptional network that controls biofilm formation by B. subtilis.

    Original languageEnglish
    Pages (from-to)6822-6832
    Number of pages11
    JournalJournal of Bacteriology
    Volume191
    Issue number22
    DOIs
    Publication statusPublished - 15 Nov 2009

    Keywords

    • TRANSITION-STATE REGULATOR
    • INTRAMEMBRANE PROTEOLYSIS
    • MULTICELLULAR BEHAVIOR
    • GENETIC COMPETENCE
    • SPORULATION GENE
    • STATIONARY-PHASE
    • STRESS RESPONSES
    • MASTER REGULATOR
    • PROTEIN
    • EXPRESSION

    Cite this

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    abstract = "A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription from these operons is repressed by two key regulators, AbrB and SinR. Relief of inhibition is necessary to allow biofilm formation to proceed. Here we present data indicating that Abh, a sequence and structural homologue of AbrB, regulates biofilm architecture by B. subtilis when colony morphology and pellicle formation are assessed. Data indicating that abh expression is dependent on the environmental signals that stimulate the activity of the extracytoplasmic function sigma-factor sigma(X) are shown. We demonstrate that expression of slrR, the proposed activator of yqxM transcription, is positively controlled by Abh. Furthermore, Abh is shown to activate transcription from the promoter of the eps operon through its control of SlrR. These findings add to the increasingly complex transcriptional network that controls biofilm formation by B. subtilis.",
    keywords = "TRANSITION-STATE REGULATOR, INTRAMEMBRANE PROTEOLYSIS, MULTICELLULAR BEHAVIOR, GENETIC COMPETENCE, SPORULATION GENE, STATIONARY-PHASE, STRESS RESPONSES, MASTER REGULATOR, PROTEIN, EXPRESSION",
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    σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh. / Murray, Ewan J.; Strauch, Mark A.; Stanley-Wall, Nicola R. (Lead / Corresponding author).

    In: Journal of Bacteriology, Vol. 191, No. 22, 15.11.2009, p. 6822-6832.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh

    AU - Murray, Ewan J.

    AU - Strauch, Mark A.

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    KW - STATIONARY-PHASE

    KW - STRESS RESPONSES

    KW - MASTER REGULATOR

    KW - PROTEIN

    KW - EXPRESSION

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