100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis

D. J. Chin; R. M. Straubinger; S. Acton; I. Näthke; F. M. Brodsky

doi:10.1073/pnas.86.23.9289

100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis

D. J. Chin, R. M. Straubinger, S. Acton, I. Näthke, F. M. Brodsky

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Abstract

The role of the 100-kDa polypeptide components of clathrin-coated vesicles in endocytosis was investigated by microinjection of specific monoclonal antibodies. Receptor-mediated uptake of transferrin and liposomes was quantitatively inhibited. These results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway. This demonstrates an in situ function of these polypeptides and the protein complexes in which they are found.

Original language	English
Pages (from-to)	9289-9293
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	23
DOIs	https://doi.org/10.1073/pnas.86.23.9289
Publication status	Published - 1989

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10.1073/pnas.86.23.9289

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abstract = "The role of the 100-kDa polypeptide components of clathrin-coated vesicles in endocytosis was investigated by microinjection of specific monoclonal antibodies. Receptor-mediated uptake of transferrin and liposomes was quantitatively inhibited. These results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway. This demonstrates an in situ function of these polypeptides and the protein complexes in which they are found.",

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AU - Chin, D. J.

AU - Straubinger, R. M.

AU - Acton, S.

AU - Näthke, I.

AU - Brodsky, F. M.

PY - 1989

Y1 - 1989

N2 - The role of the 100-kDa polypeptide components of clathrin-coated vesicles in endocytosis was investigated by microinjection of specific monoclonal antibodies. Receptor-mediated uptake of transferrin and liposomes was quantitatively inhibited. These results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway. This demonstrates an in situ function of these polypeptides and the protein complexes in which they are found.

AB - The role of the 100-kDa polypeptide components of clathrin-coated vesicles in endocytosis was investigated by microinjection of specific monoclonal antibodies. Receptor-mediated uptake of transferrin and liposomes was quantitatively inhibited. These results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway. This demonstrates an in situ function of these polypeptides and the protein complexes in which they are found.

U2 - 10.1073/pnas.86.23.9289

DO - 10.1073/pnas.86.23.9289

M3 - Article

C2 - 2574457

SN - 0027-8424

VL - 86

SP - 9289

EP - 9293

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 23

ER -

100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis

Abstract

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