11 beta-Hydroxysteroid dehydrogenase (11 beta-OHSD) protects nonspecific renal mineralocorticoid receptors from exposure to circulating glucocorticoid in vivo by catalyzing the conversion of corticosterone to inactive 11-dehydrocorticosterone. Although 11 beta-OHSD bioactivity and aldosterone binding sites are found in distal tubular cells, mineralocorticoid receptor and 11 beta-OHSD immunoreactivities are not colocalized. However, there are several kidney isoforms of 11 beta-OHSD, not all of which may be immunoreactive, whereas only a single mRNA species has been described. Using in situ hybridization we found 11 beta-OHSD mRNA is highly expressed in all renal tubular epithelia in the rat. It is therefore likely that 11 beta-OHSD is colocalized with mineralocorticoid receptors in distal tubular cells.
|Number of pages||4|
|Journal||American Journal of Physiology: Renal Physiology|
|Publication status||Published - 1 May 1991|
Yau, J. L., Van Haarst, A. D., Moisan, M. P., Fleming, S., Edwards, C. R., & Seckl, J. R. (1991). 11 beta-Hydroxysteroid dehydrogenase mRNA expression in rat kidney. American Journal of Physiology: Renal Physiology, 260(5 ), F764-767. http://ajprenal.physiology.org/content/260/5/F764.abstract