14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking

Mercedes Pozuelo-Rubio, Kathryn M. Geraghty, Barry H. C. Wong, Nicola T. Wood, David G. Campbell, Nick Morrice, Carol MacKintosh

    Research output: Contribution to journalArticlepeer-review

    357 Citations (Scopus)

    Abstract

    14-3-3-interacting proteins were isolated from extracts of proliferating HeLa cells using 14-3-3 affinity chromatography, eluting with a phosphopeptide that competes with targets for 14-3-3 binding. The isolated proteins did not bind to 14-3-3 proteins (14-3-3s) after dephosphorylation with protein phosphatase 2A (PP2A), indicating that binding to 14-3-3s requires their phosphorylation. The binding proteins identified by tryptic mass fingerprinting and Western blotting include many enzymes involved in generating precursors such as purines (AMP, GMP and ATP), FAD, NADPH, cysteine and S-adenosylmethionine, which are needed for cell growth, regulators of cell proliferation, including enzymes of DNA replication, proteins of anti-oxidative metabolism, regulators of actin dynamics and cellular trafficking, and proteins whose deregulation has been implicated in cancers, diabetes, Parkinsonism and other neurological diseases. Several proteins bound to 14-3-3–Sepharose in extracts of proliferating cells, but not in non-proliferating, serum-starved cells, including a novel microtubule-interacting protein ELP95 (EMAP-like protein of 95 kDa) and a small HVA22/Yop1p-related protein. In contrast, the interactions of 14-3-3s with the N-methyl-D-aspartate receptor 2A subunit and NuMA (nuclear mitotic apparatus protein) were not regulated by serum. Overall, our findings suggest that 14-3-3s may be central to integrating the regulation of biosynthetic metabolism, cell proliferation, survival, and other processes in human cells.
    Original languageEnglish
    Pages (from-to)395-408
    Number of pages14
    JournalBiochemical Journal
    Volume379
    Issue number2
    DOIs
    Publication statusPublished - 15 Apr 2004

    Keywords

    • Affinity chromatography
    • Cell proliferation
    • Folate metabolism
    • 14-3-3 protein
    • Proteomics

    Fingerprint Dive into the research topics of '14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking'. Together they form a unique fingerprint.

    Cite this