[19] Identification of N-terminal Myristyl Blocking Groups in Proteins

Alastair Aitken, Philip Cohen

    Research output: Contribution to journalArticle

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    Abstract

    This chapter illustrates the identification of N-terminal myristyl blocking groups in proteins. The catalytic subunit of cyclic-AMP-dependent protein kinase (C subunit) and the regulatory subunit of protein phosphatase 2B contain myristic acid covalently attached to their amino-terminus. The discovery of myristic acid at the N-terminus of a protein phosphatase and a protein kinase suggests that this unusual blocking group may be involved in the interaction of converter enzymes with their protein substrates. The myristyl group could also play a role in maintaining the subunit-subunit interactions between the regulatory (B) and catalytic (A) subunits of calcineurin and between the regulatory and catalytic subunits of cyclic-AMP-dependent protein kinase. The myristyl group may be possibly involved in the translocation of the C subunit from the cytoplasm to the nucleus, across the nuclear membrane. Fast atom bombardment (FAB) mass spectrometry is considered the most useful mass spectrometric technique for identification of myristic acid in blocked peptides. Involatile and thermally labile compounds can be studied by this method and very small amounts of material can be used.

    Original languageEnglish
    Pages (from-to)205-210
    Number of pages6
    JournalMethods in Enzymology
    Volume106
    Issue numberC
    DOIs
    Publication statusPublished - 1 Jan 1984

    Fingerprint

    Myristic Acid
    Calcineurin
    Catalytic Domain
    Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
    Fast Atom Bombardment Mass Spectrometry
    Proteins
    Phosphoprotein Phosphatases
    Nuclear Envelope
    Cyclic AMP
    Protein Kinases
    Protein Kinase C
    Mass spectrometry
    Cytoplasm
    Membranes
    Atoms
    Peptides
    Substrates
    Enzymes

    Cite this

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    abstract = "This chapter illustrates the identification of N-terminal myristyl blocking groups in proteins. The catalytic subunit of cyclic-AMP-dependent protein kinase (C subunit) and the regulatory subunit of protein phosphatase 2B contain myristic acid covalently attached to their amino-terminus. The discovery of myristic acid at the N-terminus of a protein phosphatase and a protein kinase suggests that this unusual blocking group may be involved in the interaction of converter enzymes with their protein substrates. The myristyl group could also play a role in maintaining the subunit-subunit interactions between the regulatory (B) and catalytic (A) subunits of calcineurin and between the regulatory and catalytic subunits of cyclic-AMP-dependent protein kinase. The myristyl group may be possibly involved in the translocation of the C subunit from the cytoplasm to the nucleus, across the nuclear membrane. Fast atom bombardment (FAB) mass spectrometry is considered the most useful mass spectrometric technique for identification of myristic acid in blocked peptides. Involatile and thermally labile compounds can be studied by this method and very small amounts of material can be used.",
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    [19] Identification of N-terminal Myristyl Blocking Groups in Proteins. / Aitken, Alastair; Cohen, Philip.

    In: Methods in Enzymology, Vol. 106, No. C, 01.01.1984, p. 205-210.

    Research output: Contribution to journalArticle

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    AB - This chapter illustrates the identification of N-terminal myristyl blocking groups in proteins. The catalytic subunit of cyclic-AMP-dependent protein kinase (C subunit) and the regulatory subunit of protein phosphatase 2B contain myristic acid covalently attached to their amino-terminus. The discovery of myristic acid at the N-terminus of a protein phosphatase and a protein kinase suggests that this unusual blocking group may be involved in the interaction of converter enzymes with their protein substrates. The myristyl group could also play a role in maintaining the subunit-subunit interactions between the regulatory (B) and catalytic (A) subunits of calcineurin and between the regulatory and catalytic subunits of cyclic-AMP-dependent protein kinase. The myristyl group may be possibly involved in the translocation of the C subunit from the cytoplasm to the nucleus, across the nuclear membrane. Fast atom bombardment (FAB) mass spectrometry is considered the most useful mass spectrometric technique for identification of myristic acid in blocked peptides. Involatile and thermally labile compounds can be studied by this method and very small amounts of material can be used.

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