2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.

B. S. Sproat, A. I. Lamond, R. G. Garcia, B. Beijer, U. Pieles, M. Douglas, K. Bohmann, M. Carmo-Fonseco, S. Weston, S. O'Loughlin

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.

    Original languageEnglish
    Pages (from-to)59-62
    Number of pages4
    JournalNucleic acids symposium series
    Issue number24
    Publication statusPublished - 1991

    ASJC Scopus subject areas

    • General Medicine


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