2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.

B. S. Sproat; A. I. Lamond; R. G. Garcia; B. Beijer; U. Pieles; M. Douglas; K. Bohmann; M. Carmo-Fonseco; S. Weston; S. O'Loughlin

2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.

B. S. Sproat
, A. I. Lamond
, R. G. Garcia
, B. Beijer
, U. Pieles
, M. Douglas
, K. Bohmann
, M. Carmo-Fonseco
, S. Weston
, S. O'Loughlin

Research output: Contribution to journal › Article › peer-review

Abstract

Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.

Original language	English
Pages (from-to)	59-62
Number of pages	4
Journal	Nucleic acids symposium series
Issue number	24
Publication status	Published - 1991

ASJC Scopus subject areas

General Medicine

Cite this

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title = "2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.",

abstract = "Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.",

author = "Sproat, \{B. S.\} and Lamond, \{A. I.\} and Garcia, \{R. G.\} and B. Beijer and U. Pieles and M. Douglas and K. Bohmann and M. Carmo-Fonseco and S. Weston and S. O'Loughlin",

year = "1991",

language = "English",

pages = "59--62",

journal = "Nucleic acids symposium series",

issn = "0261-3166",

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TY - JOUR

T1 - 2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.

AU - Sproat, B. S.

AU - Lamond, A. I.

AU - Garcia, R. G.

AU - Beijer, B.

AU - Pieles, U.

AU - Douglas, M.

AU - Bohmann, K.

AU - Carmo-Fonseco, M.

AU - Weston, S.

AU - O'Loughlin, S.

PY - 1991

Y1 - 1991

N2 - Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.

AB - Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been synthesized from a minimum of key intermediates. Extra protection of the lactam function in uracil and hypoxanthine proves useful. The high stability of the oligomers combined with incorporation of non-radioactive reporter groups such as fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as excellent antisense tools for studying RNA processing, for locating and visualising RNA and RNP complexes in cells, for examining splicing complexes by electron microscopy and for the affinity chromatography of RNA or RNP complexes.

UR - https://www.scopus.com/pages/publications/0026289448

M3 - Article

C2 - 1841381

AN - SCOPUS:0026289448

SN - 0261-3166

SP - 59

EP - 62

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 24

ER -

2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.

Abstract

ASJC Scopus subject areas

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