2D-DIGE as a tool in neuroproteomics

Florian Weiland (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
Place of PublicationNew York
PublisherHumana Press Inc.
Pages143-159
Number of pages17
ISBN (Electronic)9781493996629
ISBN (Print)9781493996612
DOIs
Publication statusPublished - 2019

Publication series

NameNeuromethods
Volume146
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

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Keywords

  • 2D-DIGE
  • Proteomics

Cite this

Weiland, F. (2019). 2D-DIGE as a tool in neuroproteomics. In K. W. Li (Ed.), Neuroproteomics (pp. 143-159). (Neuromethods; Vol. 146). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9662-9_13