2D-DIGE as a tool in neuroproteomics

Florian Weiland

doi:10.1007/978-1-4939-9662-9_13

2D-DIGE as a tool in neuroproteomics

Florian Weiland (Lead / Corresponding author)

MRC PPU

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

Original language	English
Title of host publication	Neuroproteomics
Editors	Ka Wan Li
Place of Publication	New York
Publisher	Humana Press Inc.
Pages	143-159
Number of pages	17
ISBN (Electronic)	9781493996629
ISBN (Print)	9781493996612
DOIs	https://doi.org/10.1007/978-1-4939-9662-9_13
Publication status	Published - 2019

Publication series

Name	Neuromethods
Volume	146
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

2D-DIGE
Proteomics

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-9662-9_13

Cite this

@inbook{1b9e9c96aae24ea8b1c78031a9729782,

title = "2D-DIGE as a tool in neuroproteomics",

abstract = "Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.",

keywords = "2D-DIGE, Proteomics",

author = "Florian Weiland",

year = "2019",

doi = "10.1007/978-1-4939-9662-9_13",

language = "English",

isbn = "9781493996612",

series = "Neuromethods",

publisher = "Humana Press Inc.",

pages = "143--159",

editor = "Li, {Ka Wan}",

booktitle = "Neuroproteomics",

address = "United States",

}

TY - CHAP

T1 - 2D-DIGE as a tool in neuroproteomics

AU - Weiland, Florian

PY - 2019

Y1 - 2019

N2 - Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

AB - Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

KW - 2D-DIGE

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=85070561864&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9662-9_13

DO - 10.1007/978-1-4939-9662-9_13

M3 - Chapter

AN - SCOPUS:85070561864

SN - 9781493996612

T3 - Neuromethods

SP - 143

EP - 159

BT - Neuroproteomics

A2 - Li, Ka Wan

PB - Humana Press Inc.

CY - New York

ER -

2D-DIGE as a tool in neuroproteomics

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this