Find Research Outputs

Search in all content

Filters for Research Outputs

Search concepts
Selected filters

Publication Year

  • 2020
  • 2019
  • 2018
  • 2017
  • 2016
  • 2015
  • 2014
  • 2013
  • 2012
  • 2011

Author

  • Dario Alessi

Accurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson's disease

Karayel, O., Tonelli, F., Virreira Winter, S., Geyer, P. E., Fan, Y., Sammler, E. M., Alessi, D., Steger, M. & Mann, M., 29 Jun 2020, In : Molecular & Cellular Proteomics.

Research output: Contribution to journalArticle

Open Access
File
6 Downloads (Pure)

Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process

Castaneda-Bueno, M., Graciela Cervantes-Perez, L., Vazquez, N., Uribe, N., Kantesaria, S., Morla, L., Bobadilla, N. A., Doucet, A., Alessi, D. R. & Gamba, G., 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 20, p. 7929-7934 6 p.

Research output: Contribution to journalArticle

156 Citations (Scopus)
Open Access
File
2 Citations (Scopus)
61 Downloads (Pure)

Akt is efficiently activated by PIF-pocket- and PtdIns(3,4,5)P-3-dependent mechanisms leading to resistance to PDK1 inhibitors

Najafov, A., Shpiro, N. & Alessi, D. R., Dec 2012, In : Biochemical Journal. 448, 2, p. 285-295 11 p.

Research output: Contribution to journalArticle

46 Citations (Scopus)

A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo

Oi, K., Sohara, E., Rai, T., Misawa, M., Chiga, M., Alessi, D. R., Sasaki, S. & Uchida, S., 15 Dec 2012, In : Open Biology. 1, 2, p. 120-127 8 p.

Research output: Contribution to journalArticle

Open Access
File
36 Citations (Scopus)
124 Downloads (Pure)

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer

Schönhuber, N., Seidler, B., Schuck, K., Veltkamp, C., Schachtler, C., Zukowska, M., Eser, S., Feyerabend, T. B., Paul, M. C., Eser, P., Klein, S., Lowy, A. M., Banerjee, R., Yang, F., Lee, C-L., Moding, E. J., Kirsch, D. G., Scheideler, A., Alessi, D. R., Varela, I. & 8 others, Bradley, A., Kind, A., Schnieke, A. E., Rodewald, H-R., Rad, R., Schmid, R. M., Schneider, G. & Saur, D., Nov 2014, In : Nature Medicine. 20, 11, p. 1340-1347 8 p.

Research output: Contribution to journalArticle

97 Citations (Scopus)

A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain

Dhekne, H. S., Yanatori, I., Gomez, R. C., Tonelli, F., Diez, F., Schüle, B., Steger, M., Alessi, D. R. & Pfeffer, S. R., 6 Nov 2018, In : eLife. 7, p. 1-26 26 p., e40202.

Research output: Contribution to journalArticle

Open Access
File
29 Citations (Scopus)
146 Downloads (Pure)

Aspirin inhibits mTOR signaling, activates AMP-activated protein kinase, and induces autophagy in colorectal cancer cells

Din, F. V. N., Valanciute, A., Dunlop, M. G., Houde, V. P., Zibrova, D., Sakamoto, K., Alessi, D. R. & Green, K. A., 2012, In : Gastroenterology. 142, 7

Research output: Contribution to journalArticle

249 Citations (Scopus)

B cell-intrinsic function of TAPP adaptors in controlling germinal center responses and autoantibody production in mice

Jayachandran, N., Landego, I., Hou, S., Alessi, D. R. & Marshall, A. J., 10 Feb 2017, In : European Journal of Immunology. 47, 2, p. 280-290 11 p.

Research output: Contribution to journalArticle

Open Access
File
5 Citations (Scopus)
172 Downloads (Pure)

Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation

Kazlauskaite, A., Martínez-Torres, R. J., Wilkie, S., Kumar, A., Peltier, J., Gonzalez, A., Johnson, C., Zhang, J., Hope, A. G., Peggie, M., Trost, M., van Aalten, D. M., Alessi, D. R., Prescott, A. R., Knebel, A., Walden, H. & Muqit, M. MK., 1 Aug 2015, In : EMBO Reports. 16, 8, p. 939-954 16 p.

Research output: Contribution to journalArticle

Open Access
File
92 Citations (Scopus)
147 Downloads (Pure)

Brain penetrant LRRK2 inhibitor

Choi, H. G., Zhang, J., Deng, X., Hatcher, J. M., Patricelli, M. P., Zhao, Z., Alessi, D. R. & Gray, N. S., 9 Aug 2012, In : ACS Medicinal Chemistry Letters. 3, 8, p. 658-662 5 p.

Research output: Contribution to journalArticle

85 Citations (Scopus)

Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2

Deng, X., Dzamko, N., Prescott, A., Davies, P., Liu, Q., Yang, Q., Lee, J-D., Patricelli, M. P., Nomanbhoy, T. K., Alessi, D. R. & Gray, N. S., Apr 2011, In : Nature Chemical Biology. 7, 4, p. 203-205 3 p.

Research output: Contribution to journalArticle

249 Citations (Scopus)

Characterization of GSK2334470, a novel and highly specific inhibitor of PDK1

Najafov, A., Sommer, E. M., Axten, J. M., DeYoung, M. P. & Alessi, D. R., 15 Jan 2011, In : Biochemical Journal. 433, p. 357-369 13 p.

Research output: Contribution to journalArticle

93 Citations (Scopus)

Characterization of TAE684 as a potent LRRK2 kinase inhibitor

Zhang, J., Deng, X., Choi, H. G., Alessi, D. R. & Gray, N. S., 1 Mar 2012, In : Bioorganic & Medicinal Chemistry Letters. 22, 5, p. 1864-1869 6 p.

Research output: Contribution to journalArticle

56 Citations (Scopus)
Open Access
File
110 Citations (Scopus)
182 Downloads (Pure)

Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases

Banerjee, S., Buhrlage, S. J., Huang, H-T., Deng, X., Zhou, W., Wang, J., Traynor, R., Prescott, A. R., Alessi, D. R. & Gray, N. S., 1 Jan 2014, In : Biochemical Journal. 457, 1, p. 215-225 11 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)

Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies

Davies, P., Hinkle, K. M., Sukar, N. N., Sepulveda, B., Mesias, R., Serrano, G., Alessi, D. R., Beach, T. G., Benson, D. L., White, C. L., Cowell, R. M., Das, S. S., West, A. B. & Melrose, H. L., 1 Jul 2013, In : Biochemical Journal. 453, 1, p. 101-113 13 p.

Research output: Contribution to journalArticle

50 Citations (Scopus)

Critical role of the SPAK protein kinase CCT domain in controlling blood pressure

Zhang, J., Siew, K., Macartney, T., O'Shaughnessy, K. M. & Alessi, D. R., 15 Aug 2015, In : Human Molecular Genetics. 24, 16, p. 4545-4558 14 p.

Research output: Contribution to journalArticle

Open Access
File
17 Citations (Scopus)
135 Downloads (Pure)

Crystal structure of the WD40 domain dimer of LRRK2

Zhang, P., Fan, Y., Ru, H., Wang, L., Magupalli, V. G., Taylor, S. S., Alessi, D. R. & Wu, H., 29 Jan 2019, In : Proceedings of the National Academy of Sciences of the United States of America. 116, 5, p. 1579-1584 6 p.

Research output: Contribution to journalArticle

Open Access
File
6 Citations (Scopus)
137 Downloads (Pure)

Deciphering the function of leucine-rich repeat kinase 2 and targeting its dysfunction in disease

Lewis, P. A. & Alessi, D. R., 2012, In : Biochemical Society Transactions. 40, 5, p. 1039-1041 3 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Design and Characterization of SGK3-PROTAC1, an Isoform Specific SGK3 Kinase PROTAC Degrader

Tovell, H., Testa, A., Zhou, H., Shpiro, N., Crafter, C., Ciulli, A. & Alessi, D., 20 Sep 2019, In : ACS Chemical Biology. 14, 9, p. 2024-2034 11 p.

Research output: Contribution to journalArticle

Open Access
File
7 Citations (Scopus)
101 Downloads (Pure)

Development of an enzyme-linked immunosorbent assay for detection of cellular and in vivo LRRK2 S935 phosphorylation

Delbroek, L., Van Kolen, K., Steegmans, L., da Cunha, R., Mandemakers, W., Daneels, G., De Bock, P-J., Zhang, J., Gevaert, K., De Strooper, B., Alessi, D. R., Verstreken, P. & Moechars, D. W., 25 Mar 2013, In : Journal of Pharmaceutical and Biomedical Analysis. 76, p. 49-58 10 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson’s disease kinase

Lis, P., Burel, S., Steger, M., Mann, M., Brown, F., Diez, F., Tonelli, F., Holton, J. L., Winglok Ho, P., Ho, S-L., Chou, M-Y., Polinski, N. K., Martinez, T. N., Davies, P. & Alessi, D. R., 2 Jan 2018, In : Biochemical Journal. 475, 1, p. 1-22 22 p.

Research output: Contribution to journalArticle

Open Access
File
31 Citations (Scopus)
290 Downloads (Pure)

Discovery of a pyrrolopyrimidine (JH-II-127), a highly potent, selective, and brain penetrant LRRK2 inhibitor

Hatcher, J. M., Zhang, J., Choi, H. G., Ito, G., Alessi, D. R. & Gray, N. S., 14 May 2015, In : ACS Medicinal Chemistry Letters. 6, 5, p. 584-589 6 p.

Research output: Contribution to journalArticle

26 Citations (Scopus)

Discovery of catalytically active orthologues of the Parkinson's disease kinase PINK1: Analysis of substrate specificity and impact of mutations

Woodroof, H. I., Pogson, J. H., Begley, M., Cantley, L. C., Deak, M., Campbell, D. G., Van Aalten, D. M. F., Whitworth, A. J., Alessi, D. R. & Muqit, M. M. K., 1 Nov 2011, In : Open Biology. 1, NOVEMBER, 110012.

Research output: Contribution to journalArticle

60 Citations (Scopus)

Discovery of potent and selective 5-azaindazole inhibitors of leucine-rich repeat kinase 2 (LRRK2) - Part 1

Osborne, J., Birchall, K., Tsagris, D. J., Lewis, S. J., Smiljanic-Hurley, E., Taylor, D. L., Levy, A., Alessi, D. & McIver, E. G., 15 Feb 2019, In : Bioorganic & Medicinal Chemistry Letters. 29, 4, p. 668-673 6 p.

Research output: Contribution to journalArticle

Open Access
File
61 Downloads (Pure)

Discovery of potent and selective covalent inhibitors of JNK

Zhang, T., Inesta-Vaquera, F., Niepel, M., Zhang, J., Ficarro, S. B., Machleidt, T., Xie, T., Marto, J. A., Kim, N., Sim, T., Laughlin, J. D., Park, H., LoGrasso, P. V., Patricelli, M., Nomanbhoy, T. K., Sorger, P. K., Alessi, D. R. & Gray, N. S., 27 Jan 2012, In : Chemistry & Biology. 19, 1, p. 140-154 15 p.

Research output: Contribution to journalArticle

177 Citations (Scopus)

Disruption of the with no lysine kinase-STE20-proline alanine-rich kinase pathway reduces the hypertension induced by angiotensin II

Graciela Cervantes-Perez, L., Castaneda-Bueno, M., Jimenez, J. V., Vazquez, N., Rojas-Vega, L., Alessi, D., Bobadilla, N. A. & Gamba, G., 1 Feb 2018, In : Journal of Hypertension. 36, 2, p. 361-367 7 p.

Research output: Contribution to journalArticle

Open Access
File
1 Citation (Scopus)
191 Downloads (Pure)

Disruptions on the highways of cellular signalling

Alessi, D., Sep 2012, In : FEBS Journal. 279, p. 23-23 1 p.

Research output: Contribution to journalMeeting abstract

Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels

Susa, K., Kita, S., Iwamoto, T., Yang, S-S., Lin, S-H., Ohta, A., Sohara, E., Rai, T., Sasaki, S., Alessi, D. R. & Uchida, S., Aug 2012, In : Clinical and Experimental Nephrology. 16, 4, p. 530-538 9 p.

Research output: Contribution to journalArticle

40 Citations (Scopus)

Effect of PI3K-and mTOR-specific inhibitors on spontaneous B-cell follicular lymphomas in PTEN/LKBI-deficient mice

Garcia-Martinez, J. M., Wullschleger, S., Preston, G., Guichard, S., Fleming, S., Alessi, D. R. & Duce, S. L., 29 Mar 2011, In : British Journal of Cancer. 104, 7, p. 1116-1125 10 p.

Research output: Contribution to journalArticle

40 Citations (Scopus)

Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors

Sommer, E. M., Dry, H., Cross, D., Guichard, S., Davies, B. R. & Alessi, D. R., 15 Jun 2013, In : Biochemical Journal. 452, 3, p. 499-508 10 p.

Research output: Contribution to journalArticle

94 Citations (Scopus)

Functional kinomics establishes a critical node of volume-sensitive cation-Cl- cotransporter regulation in the mammalian brain

Zhang, J., Gao, G., Begum, G., Wang, J., Khanna, A. R., Shmukler, B. E., Daubner, G. M., De Los Heros, P., Davies, P., Varghese, J., Bhuiyan, M. I. H., Duan, J., Zhang, J., Duran, D., Alper, S. L., Sun, D., Elledge, S. J., Alessi, D. R. & Kahle, K. T., 26 Oct 2016, In : Scientific Reports. 6, p. 1-19 19 p., 35986.

Research output: Contribution to journalArticle

Open Access
File
14 Citations (Scopus)
147 Downloads (Pure)

GSK2578215A: a potent and highly selective 2-arylmethyloxy-5-substitutent-N-arylbenzamide LRRK2 kinase inhibitor

Reith, A. D., Bamborough, P., Jandu, K., Andreotti, D., Mensah, L., Dossang, P., Choi, H. G., Deng, X., Zhang, J., Alessi, D. R. & Gray, N. S., 2012, In : Bioorganic & Medicinal Chemistry Letters. 22, 17, p. 5625-5629 5 p.

Research output: Contribution to journalArticle

96 Citations (Scopus)

Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation

Maniaci, C., Hughes, S. J., Testa, A., Chen, W., Lamont, D. J., Rocha, S., Alessi, D. R., Romeo, R. & Ciulli, A., 10 Oct 2017, In : Nature Communications. 8, p. 1-14 14 p., 830.

Research output: Contribution to journalArticle

Open Access
File
59 Citations (Scopus)
186 Downloads (Pure)

How moderate changes in Akt T-loop phosphorylation impact on tumorigenesis and insulin resistance

Wullschleger, S., Sakamoto, K., Johnstone, L., Duce, S., Fleming, S. & Alessi, D. R., Jan 2011, In : Disease Models & Mechanisms. 4, 1, p. 95-103 9 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Fan, Y., Tonelli, F., Padmanabhan, S., Baptista, M., Riley, L., Smith, D., Marras, C., Howden, A., Alessi, D. & Sammler, E., 22 Mar 2020, In : JoVE: Journal of Visualized Experiments. 157, e58956.

Research output: Contribution to journalArticle

Integrated single-cell genetic and transcriptional analysis suggests novel drivers of chronic lymphocytic leukemia

Wang, L., Fan, J., Francis, J. M., Georghiou, G., Hergert, S., Li, S., Gambe, R., Zhou, C. W., Yang, C., Xiao, S., Cin, P. D., Bowden, M., Kotliar, D., Shukla, S. A., Brown, J. R., Neuberg, D., Alessi, D. R., Zhang, C-Z., Kharchenko, P. V., Livak, K. J. & 1 others, Wu, C. J., 5 Jul 2017, In : Genome Research. 27, p. 1300-1311 11 p.

Research output: Contribution to journalArticle

Open Access
File
25 Citations (Scopus)
163 Downloads (Pure)

Interaction of TAPP adapter proteins with phosphatidylinositol (3,4)-bisphosphate regulates B cell activation and autoantibody production

Jayachandran, N., Landego, I., Gibson, I., Miller, A., Wullschleger, S., Alessi, D. & Marshall, A., 1 May 2012, In : Journal of Immunology. 188, Suppl.1, 159.5.

Research output: Contribution to journalMeeting abstract

23 Citations (Scopus)
Open Access
File
10 Citations (Scopus)
146 Downloads (Pure)

Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils

Fan, Y., Howden, A. J. M., Sarhan, A. R., Lis, P., Ito, G., Martinez, T. N., Brockmann, K., Gasser, T., Alessi, D. R. & Sammler, E., 2 Jan 2018, In : Biochemical Journal. 475, 1, p. 23-44 22 p.

Research output: Contribution to journalArticle

Open Access
File
37 Citations (Scopus)
190 Downloads (Pure)

Investigation of LKB1 Ser431 phosphorylation and Cys433 farnesylation using mouse knockin analysis reveals an unexpected role of prenylation in regulating AMPK activity

Houde, V. P., Ritorto, M. S., Gourlay, R., Davies, P., Shpiro, N., Sakamoto, K. & Alessi, D. R., 15 Feb 2014, In : Biochemical Journal. 458, 1, p. 41-56 16 p.

Research output: Contribution to journalArticle

Open Access
File
30 Citations (Scopus)
156 Downloads (Pure)

Kinase and channel activity of TRPM6 are co-ordinated by a dimerization motif and pocket interaction

van der Wijst, J., Blanchard, M. G., Woodroof, H. I., Macartney, T. J., Gourlay, R., Hoenderop, J. G., Bindels, R. J. & Alessi, D. R., 1 Jun 2014, In : Biochemical Journal. 460, 2, p. 165-175 11 p.

Research output: Contribution to journalArticle

Open Access
File
9 Citations (Scopus)
140 Downloads (Pure)

Kinase drug discovery: What's next in the field?

Cohen, P. & Alessi, D. R., 2013, In : ACS Chemical Biology. 8, 1, p. 96-104 9 p.

Research output: Contribution to journalArticle

252 Citations (Scopus)

Kinase inhibitors arrest neurodegeneration in cell and C. elegans models of LRRK2 toxicity

Yao, C., Johnson, W. M., Gao, Y., Wang, W., Zhang, J., Deak, M., Alessi, D. R., Zhu, X., Mieyal, J. J., Roder, H., Wilson-Delfosse, A. L. & Chen, S. G., 15 Jan 2013, In : Human Molecular Genetics. 22, 2, p. 328-344 17 p.

Research output: Contribution to journalArticle

51 Citations (Scopus)

LKB1 and AMPK and the cancer-metabolism link - ten years after

Hardie, D. G. & Alessi, D. R., 15 Apr 2013, In : BMC Biology. 11, p. 1-11 11 p., 36.

Research output: Contribution to journalArticle

Open Access
File
194 Citations (Scopus)
133 Downloads (Pure)

LRRK2 activation in idiopathic Parkinson's disease

Di Maio, R., Hoffman, E. K., Rocha, E. M., Keeney, M. T., Sanders, L. H., De Miranda, B. R., Zharikov, A., Van Laar, A., Stepan, A. F., Lanz, T. A., Kofler, J. K., Burton, E. A., Alessi, D. R., Hastings, T. G. & Timothy Greenamyre, J., 25 Jul 2018, In : Science Translational Medicine. 10, 451, p. 1-12 12 p., eaar5429.

Research output: Contribution to journalArticle

Open Access
File
72 Citations (Scopus)
91 Downloads (Pure)

LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages

Härtlova, A., Herbst, S., Peltier, J., Rodgers, A., Bilkei-Gorzo, O., Fearns, A., Dill, B. D., Lee, H., Flynn, R., Cowley, S. A., Davies, P., Lewis, P. A., Ganley, I. G., Martinez, J., Alessi, D. R., Reith, A. D., Trost, M. & Gutierrez, M. G., 15 Jun 2018, In : The EMBO Journal. 37, 12, p. 1-17 17 p., e98694.

Research output: Contribution to journalArticle

Open Access
File
45 Citations (Scopus)
185 Downloads (Pure)

LRRK2 kinase in Parkinson's disease

Alessi, D. R. & Sammler, E., 6 Apr 2018, In : Science. 360, 6384, p. 36-37 2 p.

Research output: Contribution to journalComment/debate

Open Access
File
62 Citations (Scopus)
385 Downloads (Pure)

Mechanism of activation of SGK3 by growth factors-via the Class 1 and Class 3 PI3Ks

Malik, N., Macartney, T., Hornberger, A., Anderson, K., Tovell, H., Prescott, A. R. & Alessi, D. R., 2 Jan 2018, In : Biochemical Journal. 475, 1, p. 117-135 19 p.

Research output: Contribution to journalArticle

Open Access
File
10 Citations (Scopus)
249 Downloads (Pure)