A catechin nanoformulation inhibits WM266 melanoma cell proliferation, migration and associated neo-angiogenesis

TY - JOUR

T1 - A catechin nanoformulation inhibits WM266 melanoma cell proliferation, migration and associated neo-angiogenesis

AU - di Leo, Nicoletta

AU - Battaglini, Matteo

AU - Berger, Liron

AU - Giannaccini, Martina

AU - Dente, Luciana

AU - Hampel, Silke

AU - Vittorio, Orazio

AU - Cirillo, Giuseppe

AU - Raffa, Vittoria

N1 - This work was supported by the EU (Marie Curie programme), by Fondazione Veronesi, by Fondazione Arpa, by Fondazione Pisa, and by the Italian “Ministero dell'Istruzione, dell'Università e della Ricerca” (PRA, progetti di ricerca di ateneo).

PY - 2017/5

Y1 - 2017/5

N2 - We validated the anticancer potential of a nanoformulation made by (+)-catechin, gelatin and carbon nanotubes in terms of inhibition of cancer cell proliferation, migration and associated neo-angiogenesis. Gelatin was selected to stabilize the catechin without compromising its anti-oxidant potential and the carbon nanotubes were used to increase its intracellular bioavailability. The anticancer potential of the resulting nanohybrid was validated on an aggressive melanoma cell line, in vitro and in zebrafish xenotransplants. The nanohybrid strongly enhances the cytotoxic effect of (+)-catechin. At a concentration of (+)-catechin 50μg/ml, the nanohybrid inhibited the ability of melanoma cells to proliferate (100% increase of cell doubling time and severe impairment in zebrafish xenotransplants), to migrate (totally inhibition in vitro and 50% reduction of cell motility in zebrafish xenotransplants) and to induce neo-angiogenesis (100% inhibition in zebrafish xenotransplants). Conversely, the free (+)-catechin and carrier (CNT:gel) had no statistically significant effects over the control, at any concentration tested. Our results suggest that the use of the nanohybrid, able to improve the therapeutic efficacy of the catechins, could represent a successful strategy for a future clinical translation.

AB - We validated the anticancer potential of a nanoformulation made by (+)-catechin, gelatin and carbon nanotubes in terms of inhibition of cancer cell proliferation, migration and associated neo-angiogenesis. Gelatin was selected to stabilize the catechin without compromising its anti-oxidant potential and the carbon nanotubes were used to increase its intracellular bioavailability. The anticancer potential of the resulting nanohybrid was validated on an aggressive melanoma cell line, in vitro and in zebrafish xenotransplants. The nanohybrid strongly enhances the cytotoxic effect of (+)-catechin. At a concentration of (+)-catechin 50μg/ml, the nanohybrid inhibited the ability of melanoma cells to proliferate (100% increase of cell doubling time and severe impairment in zebrafish xenotransplants), to migrate (totally inhibition in vitro and 50% reduction of cell motility in zebrafish xenotransplants) and to induce neo-angiogenesis (100% inhibition in zebrafish xenotransplants). Conversely, the free (+)-catechin and carrier (CNT:gel) had no statistically significant effects over the control, at any concentration tested. Our results suggest that the use of the nanohybrid, able to improve the therapeutic efficacy of the catechins, could represent a successful strategy for a future clinical translation.

KW - (+)-catechin

KW - Carbon nanotubes

KW - Melanoma cell line WM266

KW - Anti-cancer activity

KW - Anti-angiogenic activity

KW - Zebrafish

U2 - 10.1016/j.ejpb.2016.12.024

DO - 10.1016/j.ejpb.2016.12.024

M3 - Article

C2 - 28088004

SN - 0939-6411

VL - 114

SP - 1

EP - 10

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

ER -