A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

Gyorgy Hutvagner; Juanita McLachlan; Amy E. Pasquinelli; Eva Balint; Thomas Tuschl; Phillip D. Zamore

doi:10.1126/science.1062961

A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

Gyorgy Hutvagner
, Juanita McLachlan
, Amy E. Pasquinelli
, Eva Balint
, Thomas Tuschl
, Phillip D. Zamore

Research output: Contribution to journal › Article › peer-review

2340 Citations (Scopus)

Abstract

The 21-nucleotide small temporal RNA (stRNA) let-7regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

Original language	English
Pages (from-to)	834-838
Number of pages	5
Journal	Science
Volume	293
Issue number	5531
DOIs	https://doi.org/10.1126/science.1062961
Publication status	Published - 2001

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title = "A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA",

abstract = "The 21-nucleotide small temporal RNA (stRNA) let-7regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.",

author = "Gyorgy Hutvagner and Juanita McLachlan and Pasquinelli, \{Amy E.\} and Eva Balint and Thomas Tuschl and Zamore, \{Phillip D.\}",

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T1 - A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

AU - Hutvagner, Gyorgy

AU - McLachlan, Juanita

AU - Pasquinelli, Amy E.

AU - Balint, Eva

AU - Tuschl, Thomas

AU - Zamore, Phillip D.

N1 - dc.publisher: American Association for the Advancement of Science

PY - 2001

Y1 - 2001

N2 - The 21-nucleotide small temporal RNA (stRNA) let-7regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

AB - The 21-nucleotide small temporal RNA (stRNA) let-7regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

U2 - 10.1126/science.1062961

DO - 10.1126/science.1062961

M3 - Article

SN - 0036-8075

VL - 293

SP - 834

EP - 838

JO - Science

JF - Science

IS - 5531

ER -

A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

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